BRENDA - Enzyme Database show
show all sequences of 1.1.1.47

Cooperative effect of two surface amino acid mutations (Q252L and E170K) in glucose dehydrogenase from Bacillus megaterium IWG3 on stabilization of its oligomeric state

Baik, S.H.; Michel, F.; Aghajari, N.; Haser, R.; Harayama, S.; Appl. Environ. Microbiol. 71, 3285-3293 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli strain JM109
Bacillus megaterium
Engineering
Amino acid exchange
Commentary
Organism
E170K
mutant is unstable at an alkaline pH (26% residual activity at pH 10-10.5), dissociates into dimers at an alkaline pH
Bacillus megaterium
Q252L
mutant is inactivated at pH values above 9, dissociates into dimers at an alkaline pH
Bacillus megaterium
Q252L/E170K
mutant exhibits increased pH stability (95% residual activity at pH 8-10.5) in the absence of NaCl
Bacillus megaterium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
NAD+
apparent value, mutant enzyme Q252L, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
0.17
-
NAD+
apparent value, wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
0.22
-
NAD+
apparent value, mutant enzyme E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
0.33
-
NAD+
apparent value, mutant enzyme Q252L/E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
7.5
-
D-glucose
apparent value, mutant enzyme E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
8
-
D-glucose
apparent value, mutant enzyme Q252L, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
8.5
-
D-glucose
apparent value, mutant enzyme Q252L/E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
14
-
D-glucose
apparent value, wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus megaterium
P40288
strain IGW3
-
Bacillus megaterium IGW3
P40288
strain IGW3
-
Purification (Commentary)
Commentary
Organism
high-pressure liquid chromatography
Bacillus megaterium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose + NAD+
-
677657
Bacillus megaterium
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
gel filtration
Bacillus megaterium
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
317
-
D-glucose
apparent value, mutant enzyme Q252L, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
334
-
D-glucose
apparent value, mutant enzyme Q252L/E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
352
-
D-glucose
apparent value, mutant enzyme E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
395
-
D-glucose
apparent value, wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
9
wild type GlcDH shows reversible dissociation-association between inactive monomers and active tetramers when the pH is shifted between 9 and 7
Bacillus megaterium
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Bacillus megaterium
NADP+
-
Bacillus megaterium
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli strain JM109
Bacillus megaterium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Bacillus megaterium
NADP+
-
Bacillus megaterium
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E170K
mutant is unstable at an alkaline pH (26% residual activity at pH 10-10.5), dissociates into dimers at an alkaline pH
Bacillus megaterium
Q252L
mutant is inactivated at pH values above 9, dissociates into dimers at an alkaline pH
Bacillus megaterium
Q252L/E170K
mutant exhibits increased pH stability (95% residual activity at pH 8-10.5) in the absence of NaCl
Bacillus megaterium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
NAD+
apparent value, mutant enzyme Q252L, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
0.17
-
NAD+
apparent value, wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
0.22
-
NAD+
apparent value, mutant enzyme E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
0.33
-
NAD+
apparent value, mutant enzyme Q252L/E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
7.5
-
D-glucose
apparent value, mutant enzyme E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
8
-
D-glucose
apparent value, mutant enzyme Q252L, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
8.5
-
D-glucose
apparent value, mutant enzyme Q252L/E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
14
-
D-glucose
apparent value, wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
Purification (Commentary) (protein specific)
Commentary
Organism
high-pressure liquid chromatography
Bacillus megaterium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glucose + NAD+
-
677657
Bacillus megaterium
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
gel filtration
Bacillus megaterium
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
317
-
D-glucose
apparent value, mutant enzyme Q252L, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
334
-
D-glucose
apparent value, mutant enzyme Q252L/E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
352
-
D-glucose
apparent value, mutant enzyme E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
395
-
D-glucose
apparent value, wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 25C
Bacillus megaterium
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
9
wild type GlcDH shows reversible dissociation-association between inactive monomers and active tetramers when the pH is shifted between 9 and 7
Bacillus megaterium
Other publictions for EC 1.1.1.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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-
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1
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1
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1
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1
1
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724972
Nishioka
Structure-guided mutagenesis f ...
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279
3264-3275
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-
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1
1
6
-
-
14
-
-
-
-
-
3
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-
-
-
-
-
-
-
10
-
1
-
1
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-
1
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-
-
-
-
2
-
2
6
-
-
-
-
14
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-
-
-
-
-
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10
-
2
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2
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718811
Haferkamp
An additional glucose dehydrog ...
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Biochem. Soc. Trans.
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2
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1
2
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-
-
-
2
-
2
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-
-
2
-
-
-
1
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-
-
-
6
-
1
-
-
2
1
-
-
-
-
1
1
-
2
2
734842
Haferkamp
-
Biochemical studies of enzymes ...
Sulfolobus solfataricus, Sulfolobus solfataricus DSM 1617
PH. D. Thesis Universitt Duisburg-Essen
2011
0000
2011
-
-
1
-
-
-
-
7
-
-
2
-
-
2
-
-
-
-
-
-
-
-
8
1
-
-
-
8
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
-
-
8
1
-
-
-
8
-
-
-
-
-
-
-
-
8
8
711346
Senesi
Hexose-6-phosphate dehydrogena ...
Homo sapiens, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
Biol. Chem.
391
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3
4
-
2
-
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5
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1
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4
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4
4
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-
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4
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2
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1
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-
1
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-
8
4
-
13
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-
8
4
-
-
1
-
-
-
-
2
14
1
1
-
1
3
1
1
3
-
-
-
-
1
-
1
-
-
-
-
-
8
-
4
-
13
3
-
8
-
-
1
-
-
-
2
14
1
1
-
1
3
1
1
3
-
-
-
-
-
-
-
711897
Alcina
Hexose-6-phosphate dehydrogena ...
Homo sapiens
Eur. J. Hum. Genet.
18
618-620
2010
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
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-
-
-
-
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
713490
Rogoff
Contribution of hexose-6-phosp ...
Mus musculus
Redox Rep.
15
64-70
2010
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725684
Park
Enantioselective bioconversion ...
Bacillus subtilis
J. Microbiol. Biotechnol.
20
1300-6
2010
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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1
-
1
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1
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1
1
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-
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-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
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-
712633
Gomez-Sanchez
Immunohistochemical demonstrat ...
Rattus norvegicus
J. Histochem. Cytochem.
57
633-641
2009
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
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3
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1
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1
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1
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3
-
-
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1
-
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-
-
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-
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-
684413
Muratsubaki
An enzyme cycling method for m ...
Bacillus megaterium
Anal. Biochem.
378
65-70
2008
-
1
-
-
-
-
-
-
-
-
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2
-
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-
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1
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-
684178
Kanaujia
Preliminary X-ray crystallogra ...
Thermus thermophilus HB8
Acta Crystallogr. Sect. F
63
446-448
2007
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
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1
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1
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686083
Vazquez-Figueroa
Development of a thermostable ...
Bacillus licheniformis, Bacillus subtilis subsp. subtilis 168, Bacillus subtilis subsp. subtilis, Bacillus thuringiensis
ChemBioChem
8
2295-2301
2007
-
-
-
-
42
-
-
-
-
-
-
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-
5
-
-
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-
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17
-
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42
-
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-
-
-
17
-
-
-
-
-
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688166
Xu
High-level expression of recom ...
Bacillus megaterium, Bacillus megaterium AS1.223
J. Ind. Microbiol. Biotechnol.
34
83-90
2007
-
1
1
-
-
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-
-
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5
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1
1
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668349
Luna
Glucose metabolism in batch an ...
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus PAL 3
Curr. Microbiol.
52
163-168
2006
-
-
-
-
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2
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4
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7
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4
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1
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7
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4
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669452
Milburn
The structural basis of substr ...
Sulfolobus solfataricus
J. Biol. Chem.
281
14796-14804
2006
-
-
1
1
2
-
-
18
-
-
2
-
-
1
-
-
1
-
-
-
-
-
4
1
-
-
-
18
-
-
-
2
-
-
-
-
-
1
2
1
2
-
-
-
-
18
-
-
2
-
-
-
-
1
-
-
-
-
4
1
-
-
-
18
-
-
-
-
-
-
-
-
-
-
728653
Britton
Analysis of protein solvent in ...
Haloferax mediterranei, Haloferax mediterranei DSM 1411
Proc. Natl. Acad. Sci. USA
103
4846-4851
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
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-
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667087
Theodossis
Preliminary crystallographic s ...
Sulfolobus solfataricus
Acta Crystallogr. Sect. F
F61
112-115
2005
-
-
1
1
-
-
-
-
-
-
2
-
-
3
-
-
1
-
-
-
-
-
2
1
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-
-
-
-
2
-
-
-
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-
1
2
1
-
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-
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2
-
-
-
-
1
-
-
-
-
2
1
-
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-
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668064
Yun
Stereospecific synthesis of (R ...
Bacillus subtilis
Biotechnol. Prog.
21
366-371
2005
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668130
Sharma
Substrate specificity of gluco ...
Enterobacter asburiae, Enterobacter asburiae PSI3
Can. J. Microbiol.
51
477-482
2005
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677657
Baik
Cooperative effect of two surf ...
Bacillus megaterium, Bacillus megaterium IGW3
Appl. Environ. Microbiol.
71
3285-3293
2005
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654400
Pire
-
Stability and enzymatic studie ...
Haloferax mediterranei
Biocatal. Biotransform.
22
17-23
2004
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654301
Baik
Significantly enhanced stabili ...
Bacillus licheniformis, Bacillus megaterium, Bacillus subtilis
Appl. Microbiol. Biotechnol.
61
329-335
2003
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3
3
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654857
Inose
Cloning and expression of the ...
Burkholderia cepacia
Biochim. Biophys. Acta
1645
133-138
2003
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656156
Lamble
Metabolic pathway promiscuity ...
Sulfolobus solfataricus
J. Biol. Chem.
278
34066-34072
2003
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656567
Ohshima
-
The Sulfolobus tokodaii gene S ...
Sulfolobus tokodaii, Sulfolobus tokodaii 7
J. Mol. Catal. B
23
281-289
2003
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3
1
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9
1
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2
2
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16
1
1
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2
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1
1
1
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639095
Yamamoto
Crystal structure of glucose d ...
Bacillus megaterium, Bacillus megaterium IWG3
J. Biochem.
129
303-312
2001
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677305
Yamamoto
Crystallization and preliminar ...
Bacillus megaterium, Bacillus megaterium IWG3
Acta Crystallogr. Sect. D
56
1443-1445
2000
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1
1
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8
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639096
Bhaumik
Kinetic mechanism of glucose d ...
Halobacterium salinarum
Indian J. Biochem. Biophys.
36
143-149
1999
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4
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285784
Kataoka
Escherichia coli transformant ...
Bacillus megaterium, Bacillus megaterium IWG3
Biosci. Biotechnol. Biochem.
62
167-169
1998
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1
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8
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639097
Siebers
Carbohydrate metabolism in the ...
Thermoproteus tenax
Arch. Microbiol.
168
120-127
1997
1
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4
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639099
John
-
The crystal structure of gluco ...
Thermoplasma acidophilum
Structure
15
385-393
1994
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1
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-
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639100
Tranulis
Glucose dehydrogenase in beef ...
Bos taurus, Oncorhynchus mykiss
Comp. Biochem. Physiol. B
109
427-435
1994
-
-
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-
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4
2
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2
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1
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9
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639101
Nagao
Cloning, nucleotide sequences, ...
Bacillus megaterium
J. Bacteriol.
174
5013-5020
1992
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1
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16
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3
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7
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9
1
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7
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5
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639102
Bright
-
Crystallization and preliminar ...
Thermoplasma acidophilum
J. Mol. Biol.
22
143-144
1991
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1
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-
639103
Hilt
Glucose dehydrogenase from Bac ...
Bacillus subtilis
Biochim. Biophys. Acta
1076
298-304
1991
-
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1
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2
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1
1
1
-
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-
1
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2
-
-
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-
-
-
-
639104
Persson
Purification and site-specific ...
Bacillus subtilis
FEBS Lett.
270
41-44
1990
-
-
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1
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1
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1
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1
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-
-
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-
639105
Yamamot
Characterization of mutant glu ...
Bacillus megaterium
Ann. N. Y. Acad. Sci.
613
362-365
1990
-
-
1
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5
-
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24
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1
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2
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5
22
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5
2
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1
2
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5
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24
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2
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5
22
-
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5
-
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-
-
639106
Smith
Purification and characterizat ...
Thermoplasma acidophilum
Biochem. J.
261
973-977
1989
-
-
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1
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2
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2
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3
1
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1
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1
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2
1
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2
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2
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1
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2
1
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2
-
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-
-
-
679615
Mitamura
Enzymatic properties of isozym ...
Bacillus megaterium, Bacillus megaterium IAM 1030, Bacillus megaterium IWG3
Eur. J. Biochem.
186
389-393
1989
-
-
1
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2
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14
-
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1
-
-
-
-
-
39
-
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1
2
1
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1
-
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3
3
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6
-
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3
-
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-
-
39
-
-
-
3
6
3
-
-
-
-
-
-
-
-
-
680592
Makino
Stability-increasing mutants o ...
Bacillus megaterium, Bacillus megaterium IWG3
J. Biol. Chem.
264
6381-6385
1989
-
-
1
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8
-
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1
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8
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1
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2
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1
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1
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8
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1
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2
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-
639107
Carper
Affinity chromatography of glu ...
Sus scrofa
Experientia
44
29-32
1988
-
-
-
-
-
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4
1
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1
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1
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1
1
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3
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1
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1
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1
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4
1
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1
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1
1
-
3
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
679613
Heilmann
Identification and isolation o ...
Bacillus megaterium, Bacillus megaterium M1286
Eur. J. Biochem.
174
485-490
1988
-
-
1
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2
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10
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2
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1
2
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-
10
-
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-
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-
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-
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-
639108
Maurer
Reversible pH-induced dissocia ...
Bacillus megaterium
Z. Naturforsch. C
42
907-915
1987
-
-
-
-
-
-
1
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1
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2
2
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2
2
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2
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1
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2
2
-
-
-
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-
2
-
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-
-
-
639109
Hnes
An integrated prediction of se ...
Bacillus megaterium
FEBS Lett.
212
193-198
1987
-
-
-
-
-
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2
-
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1
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2
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2
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2
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2
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2
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679611
Pal
Crystallization of and X-ray i ...
Bacillus megaterium, Bacillus megaterium MI286
Eur. J. Biochem.
167
123-124
1987
-
-
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1
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1
2
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2
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2
1
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2
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2
1
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1
2
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-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639110
Giardina
Glucose dehydrogenase from the ...
Sulfolobus solfataricus
Biochem. J.
239
517-522
1986
-
-
-
-
-
4
4
7
-
4
3
-
3
1
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1
-
-
-
1
1
21
1
1
1
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-
1
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2
-
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2
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4
-
4
-
7
-
4
3
-
3
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1
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1
1
21
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
639111
Juhasz
-
The pyridine nucleotide-depend ...
Nostoc sp., Nostoc sp. MAC
FEBS Lett.
194
121-125
1986
-
-
-
-
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1
4
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4
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1
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1
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4
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2
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2
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1
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4
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1
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1
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4
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-
-
639112
Maurer
-
Reversible pH-induced dissocia ...
Bacillus megaterium
Biochim. Biophys. Acta
827
381-388
1985
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1
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-
1
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1
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-
679608
Froeschle
Tyrosine modification of gluco ...
Bacillus megaterium, Bacillus megaterium IWG3
Eur. J. Biochem.
142
533-540
1984
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-
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1
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1
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8
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1
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2
1
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1
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639113
Strauss
-
Role of glucose dehydrogenase ...
Bacillus subtilis, Bacillus subtilis NS168I-2
FEMS Microbiol. Lett.
20
379-384
1983
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2
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2
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1
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4
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639114
Carper
Characterization studies of gl ...
Sus scrofa
Experientia
39
1295-1297
1983
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12
1
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1
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1
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4
1
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1
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12
1
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1
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1
1
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4
1
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1
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639115
Ramaley
Glycerol protection and purifi ...
Bacillus subtilis
J. Biol. Chem.
258
12558-12565
1983
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-
-
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1
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3
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2
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2
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1
1
1
3
1
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1
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2
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1
1
1
3
1
-
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1
1
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639116
Thompson
Guanidinium- and temperature-i ...
Sus scrofa
Biochim. Biophys. Acta
745
279-284
1983
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1
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1
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1
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679607
Ulmer
Evidence for an essential hist ...
Bacillus megaterium, Bacillus megaterium IWG3
Eur. J. Biochem.
136
183-194
1983
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1
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8
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2
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2
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639117
Adachi
-
D-glucose dehydrogenase from G ...
Gluconobacter oxydans
Methods Enzymol.
89
159-163
1982
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1
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4
2
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2
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1
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1
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4
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1
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1
1
1
1
1
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1
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-
639118
Kobayashi
-
Comparative studies of NAD+-de ...
Corynebacterium sp., Corynebacterium sp. 150-1, Corynebacterium sp. 93-1
Agric. Biol. Chem.
46
2139-2142
1982
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10
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4
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5
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15
1
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2
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2
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2
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10
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4
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15
1
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2
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2
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639119
Campbell
Bovine liver glucose dehydroge ...
Bos taurus
Arch. Biochem. Biophys.
215
289-301
1982
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5
1
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2
1
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1
1
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3
1
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1
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5
1
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2
1
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1
1
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3
1
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1
1
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285782
Adachi
-
Crystallization and characteri ...
Gluconobacter oxydans
Agric. Biol. Chem.
44
301-308
1980
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1
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4
2
1
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2
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1
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3
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2
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1
3
1
1
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1
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639120
Kobayashi
-
NAD-dependent maltose dehydrog ...
Corynebacterium sp., Corynebacterium sp. 150-1
Agric. Biol. Chem.
44
2837-2841
1980
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3
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2
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1
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639121
Kobayashi
-
Purification and properties of ...
Corynebacterium sp., Corynebacterium sp. 93-1
Agric. Biol. Chem.
44
2261-2269
1980
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18
5
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2
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3
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1
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12
1
1
1
2
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1
1
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1
1
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18
1
5
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1
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1
1
12
1
1
1
2
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1
1
1
1
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639123
Yokota
-
Reactivation of inactivated D- ...
Bacillus sp., Bacillus sp. BG 1722
Agric. Biol. Chem.
43
271-278
1979
8
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8
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1
10
2
1
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1
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639124
Fujita
Location and properties of glu ...
Bacillus subtilis
J. Bacteriol.
132
282-293
1977
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5
1
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1
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1
1
3
-
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1
1
2
1
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639125
Pauly
Conformational and functional ...
Bacillus megaterium
Biochemistry
16
4599-4604
1977
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2
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1
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6
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6
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639126
Pauly
D-Glucose dehydrogenase from B ...
Bacillus megaterium
Hoppe-Seyler's Z. Physiol. Chem.
356
1613-1623
1975
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1
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4
1
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2
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2
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639127
Pulich
Pyridine nucleotide-dependent ...
Chlorogloeopsis fritschii, Lyngbya lagerheimii, Nostoc sp., Schizothrix calcicola
J. Biol. Chem.
114
28-33
1973
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4
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6
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6
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6
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639128
Shatton
Glucose (hexose 6-phosphate) d ...
Oncorhynchus mykiss
J. Biol. Chem.
246
4878-4885
1971
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25
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1
1
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1
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1
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1
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11
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2
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1
1
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2
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25
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1
1
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1
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1
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11
-
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2
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1
1
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639129
Thompson
Glucose dehydrogenase from pig ...
Sus scrofa
Biochim. Biophys. Acta
198
397-406
1970
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1
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4
2
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1
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1
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1
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1
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4
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1
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1
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1
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