Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM109 | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
E170K | mutant is unstable at an alkaline pH (26% residual activity at pH 10-10.5), dissociates into dimers at an alkaline pH | Priestia megaterium |
Q252L | mutant is inactivated at pH values above 9, dissociates into dimers at an alkaline pH | Priestia megaterium |
Q252L/E170K | mutant exhibits increased pH stability (95% residual activity at pH 8-10.5) in the absence of NaCl | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
NAD+ | apparent value, mutant enzyme Q252L, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
0.17 | - |
NAD+ | apparent value, wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
0.22 | - |
NAD+ | apparent value, mutant enzyme E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
0.33 | - |
NAD+ | apparent value, mutant enzyme Q252L/E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
7.5 | - |
D-glucose | apparent value, mutant enzyme E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
8 | - |
D-glucose | apparent value, mutant enzyme Q252L, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
8.5 | - |
D-glucose | apparent value, mutant enzyme Q252L/E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
14 | - |
D-glucose | apparent value, wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | P40288 | - |
- |
Priestia megaterium IGW3 | P40288 | - |
- |
Purification (Comment) | Organism |
---|---|
high-pressure liquid chromatography | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + NAD+ | - |
Priestia megaterium | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | - |
Priestia megaterium IGW3 | D-glucono-1,5-lactone + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | gel filtration | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
GlcDH | - |
Priestia megaterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
317 | - |
D-glucose | apparent value, mutant enzyme Q252L, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
334 | - |
D-glucose | apparent value, mutant enzyme Q252L/E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
352 | - |
D-glucose | apparent value, mutant enzyme E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
395 | - |
D-glucose | apparent value, wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | wild type GlcDH shows reversible dissociation-association between inactive monomers and active tetramers when the pH is shifted between 9 and 7 | Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Priestia megaterium | |
NADP+ | - |
Priestia megaterium |