Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 60% inhibition at 10 mM | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
NADP+ | with beta-D-galactose, pH 7.5, 70°C | Saccharolobus solfataricus | |
0.57 | - |
NAD+ | with beta-D-galactose, pH 7.5, 70°C | Saccharolobus solfataricus | |
1.3 | - |
NADP+ | with beta-D-glucose, pH 7.5, 70°C | Saccharolobus solfataricus | |
1.5 | - |
NAD+ | with beta-D-glucose, pH 7.5, 70°C | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by 0.1 mM CaCl2 and MgCl2 | Saccharolobus solfataricus | |
Zn2+ | enzyme contains the catalytic zinc binding residues Cys39 and His66, no increase in activity by addition of 0.1 mM ZnCl2 | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
- |
Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + NAD(P)+ | Saccharolobus solfataricus | first step of the non-phosphorylative Entner-Doudoroff pathway | D-glucono-1,5-lactone + NAD(P)H + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | O93715 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by gel filtration, recombinant enzyme from Escherichia coli by heat denaturation, and affinity gel chromatography | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + NAD(P)+ | low activity | Saccharolobus solfataricus | ? | - |
? | |
6-deoxy-D-glucose + NAD(P)+ | - |
Saccharolobus solfataricus | ? | - |
? | |
beta-D-glucose + NAD(P)+ | - |
Saccharolobus solfataricus | D-glucono-1,5-lactone + NAD(P)H + H+ | - |
ir | |
beta-D-glucose + NAD(P)+ | first step of the non-phosphorylative Entner-Doudoroff pathway | Saccharolobus solfataricus | D-glucono-1,5-lactone + NAD(P)H + H+ | - |
ir | |
D-fucose + NAD(P)+ | - |
Saccharolobus solfataricus | ? | - |
? | |
D-galactose + NAD(P)+ | equally active compared to beta-D-glucose | Saccharolobus solfataricus | D-galactono-1,5-lactone + NAD(P)H | - |
? | |
D-glucosamine + NAD(P)+ | low activity | Saccharolobus solfataricus | ? | - |
? | |
D-idose + NAD(P)+ | low activity | Saccharolobus solfataricus | ? | - |
? | |
D-lyxose + NAD(P)+ | low activity | Saccharolobus solfataricus | ? | - |
? | |
D-xylose + NAD(P)+ | - |
Saccharolobus solfataricus | ? | - |
? | |
L-arabinose + NAD(P)+ | - |
Saccharolobus solfataricus | ? | - |
? | |
additional information | D-ribose, maltose, lactose and sucrose are poor substrates, no activity with D-allose, D-arabinose, and D-mannose | Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
glucose dehydrogenase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37.4 | - |
NADP+ | with beta-D-galactose, pH 7.5, 70°C | Saccharolobus solfataricus | |
47.7 | - |
NADP+ | with beta-D-glucose, pH 7.5, 70°C | Saccharolobus solfataricus | |
61.3 | - |
NAD+ | with beta-D-galactose, pH 7.5, 70°C | Saccharolobus solfataricus | |
74.9 | - |
NAD+ | with beta-D-glucose, pH 7.5, 70°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | dual cofactor specificity due to presence of Asn215, His217, and the GXGXXA motif | Saccharolobus solfataricus | |
NAD+ | - |
Saccharolobus solfataricus | |
NADP+ | - |
Saccharolobus solfataricus |