Literature summary for 1.1.1.47 extracted from

Bhaumik, S.R.; Sonawat, H.M.
Kinetic mechanism of glucose dehydrogenase from Halobacterium salinarum (1999), Indian J. Biochem. Biophys., 36, 143-149.

Search for more literature for 1.1.1.47

Inhibitors

Inhibitors	Comment	Organism	Structure
D-glucono-1,5-lactone	forward reaction, oxidation, product inhibition, noncompetitive vs. beta-D-glucose and NADP+	Halobacterium salinarum
NADP+	reverse reaction, reduction, product inhibition, competitive vs. NADPH and noncompetitive vs. D-glucono-1-5-lactone	Halobacterium salinarum
NADPH	product inhibition, competitive with respect to NADP	Halobacterium salinarum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.14	-	beta-D-glucose	reduction, reverse reaction	Halobacterium salinarum
0.19	-	NADP+	-	Halobacterium salinarum
0.49	-	NADPH	-	Halobacterium salinarum
2.57	-	beta-D-glucose	oxidation, forward reaction	Halobacterium salinarum

Organism

Organism	UniProt	Comment	Textmining
Halobacterium salinarum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate, Sepharose 6B, DEAE-Sepharose, hydroxyapatite chromatography	Halobacterium salinarum

Reaction

Reaction	Comment	Organism	Reaction ID
D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H + H+	ordered bi bi mechanism, formation of ternary complexes, NADP+ adds first, NADPH formed dissociates last, for reverse reaction, NADPH adds first, NADP+ leaves last	Halobacterium salinarum	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-D-glucose + NADP+	-	Halobacterium salinarum	D-glucono-1,5-lactone + NADPH + H+	-	r

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Halobacterium salinarum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	-	Halobacterium salinarum

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Release 2023.1 (January 2023)