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show all sequences of 1.1.1.45

Subunit structure and kinetic properties of L-beta-hydroxyacid dehydrogenase of Drosophila

Cannistraro, V.J.; Borack, L.I.; Chase, T.; Biochim. Biophys. Acta 569, 1-5 (1979)

Data extracted from this reference:

General Stability
General Stability
Organism
3 M urea results in loss of activity of 83% in 7 min
Drosophila melanogaster
Inhibitors
Inhibitors
Commentary
Organism
Structure
acetoacetate
competitive inhibitor
Drosophila melanogaster
NADH
competitive inhibitor
Drosophila melanogaster
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
NAD+
cosubstrate L-gulonate, pH 8.2
Drosophila melanogaster
0.13
-
NAD+
cosubstrate L-gulonate, pH 8.2
Drosophila melanogaster
0.85
-
L-gulonate
cofactor NAD+, pH 8.2
Drosophila melanogaster
14.9
-
L-3-hydroxybutanoate
cofactor NAD+, pH 8.2
Drosophila melanogaster
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23500
-
alphabeta, 1 * 23500 + 1 * 40000, SDS-PAGE
Drosophila melanogaster
40000
-
alphabeta, 1 * 23500 + 1 * 40000, SDS-PAGE
Drosophila melanogaster
63500
-
SDS-PAGE
Drosophila melanogaster
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-gulonic acid + NAD+
Drosophila melanogaster
-
3-dehydro-L-gulonic acid + NADH + H+
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Drosophila melanogaster
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
12
-
-
Drosophila melanogaster
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-3-hydroxybutanoate + NADH
-
286910
Drosophila melanogaster
acetoacetate + NAD+
-
-
-
-
L-gulonic acid + NAD+
-
286910
Drosophila melanogaster
3-dehydro-L-gulonic acid + NADH + H+
-
-
-
-
L-gulonic acid + NAD+
-
286910
Drosophila melanogaster
3-dehydro-L-gulonic acid + NADH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
alphabeta, 1 * 23500 + 1 * 40000, SDS-PAGE
Drosophila melanogaster
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.733
-
DL-3-hydroxybutanoate
-
Drosophila melanogaster
7.4
-
L-gulonate
-
Drosophila melanogaster
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Drosophila melanogaster
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Drosophila melanogaster
NADH
-
Drosophila melanogaster
NADP+
10% activity of NAD+
Drosophila melanogaster
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Drosophila melanogaster
NADH
-
Drosophila melanogaster
NADP+
10% activity of NAD+
Drosophila melanogaster
General Stability (protein specific)
General Stability
Organism
3 M urea results in loss of activity of 83% in 7 min
Drosophila melanogaster
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetoacetate
competitive inhibitor
Drosophila melanogaster
NADH
competitive inhibitor
Drosophila melanogaster
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
NAD+
cosubstrate L-gulonate, pH 8.2
Drosophila melanogaster
0.13
-
NAD+
cosubstrate L-gulonate, pH 8.2
Drosophila melanogaster
0.85
-
L-gulonate
cofactor NAD+, pH 8.2
Drosophila melanogaster
14.9
-
L-3-hydroxybutanoate
cofactor NAD+, pH 8.2
Drosophila melanogaster
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23500
-
alphabeta, 1 * 23500 + 1 * 40000, SDS-PAGE
Drosophila melanogaster
40000
-
alphabeta, 1 * 23500 + 1 * 40000, SDS-PAGE
Drosophila melanogaster
63500
-
SDS-PAGE
Drosophila melanogaster
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-gulonic acid + NAD+
Drosophila melanogaster
-
3-dehydro-L-gulonic acid + NADH + H+
-
-
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
12
-
-
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-3-hydroxybutanoate + NADH
-
286910
Drosophila melanogaster
acetoacetate + NAD+
-
-
-
-
L-gulonic acid + NAD+
-
286910
Drosophila melanogaster
3-dehydro-L-gulonic acid + NADH + H+
-
-
-
-
L-gulonic acid + NAD+
-
286910
Drosophila melanogaster
3-dehydro-L-gulonic acid + NADH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
alphabeta, 1 * 23500 + 1 * 40000, SDS-PAGE
Drosophila melanogaster
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.733
-
DL-3-hydroxybutanoate
-
Drosophila melanogaster
7.4
-
L-gulonate
-
Drosophila melanogaster
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Drosophila melanogaster
Other publictions for EC 1.1.1.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712765
Asada
Dimeric crystal structure of r ...
Oryctolagus cuniculus
J. Mol. Biol.
401
906-920
2010
-
-
1
1
4
-
-
17
-
-
1
1
-
2
-
-
1
1
-
2
-
-
4
2
1
-
-
-
1
-
-
4
-
-
-
-
-
1
4
1
4
-
-
-
-
17
-
-
1
1
-
-
-
1
-
2
-
-
4
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
695345
Asada
Crystallization and preliminar ...
Oryctolagus cuniculus
Acta Crystallogr. Sect. F
64
228-230
2008
-
-
1
1
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669147
Ishikura
Structural and functional char ...
Oryctolagus cuniculus
J. Biochem.
137
303-314
2005
-
-
1
-
6
-
5
19
1
-
3
-
-
3
-
-
1
-
-
2
2
-
5
1
-
-
-
-
1
-
-
2
-
1
2
-
-
1
2
-
6
-
2
5
-
19
1
-
3
-
-
-
-
1
-
2
2
-
5
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
286146
Goode
Accumulation of xylitol in the ...
Bos taurus, Rattus norvegicus
FEBS Lett.
395
174-178
1996
-
2
-
-
-
-
-
-
-
-
2
2
-
2
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286911
Menotti-Raymond
Further characterization of L- ...
Drosophila melanogaster
Biochim. Biophys. Acta
841
15-21
1985
-
-
-
-
-
-
-
-
-
-
1
1
-
3
-
1
1
-
-
-
2
-
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
1
-
-
1
1
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286910
Cannistraro
Subunit structure and kinetic ...
Drosophila melanogaster
Biochim. Biophys. Acta
569
1-5
1979
-
-
-
-
-
1
2
4
-
-
3
1
-
2
-
-
-
-
-
-
1
-
3
1
-
-
-
2
1
-
-
3
-
-
-
-
-
-
3
-
-
1
-
2
-
4
-
-
3
1
-
-
-
-
-
-
1
-
3
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
286909
Borack
-
Organ distribution of Drosophi ...
Drosophila melanogaster
Experientia
30
31
1974
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286908
Koundakjian
3-Hydroxy acid dehydrogenases ...
Ovis aries
Biochem. J.
127
449-452
1972
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286907
Borack
Drosophila beta-L-hydroxy acid ...
Drosophila melanogaster
J. Biol. Chem.
246
5345-5350
1970
-
-
-
-
-
-
-
3
-
-
1
1
-
1
-
-
1
-
-
-
1
-
4
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
1
1
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
286906
Dworsky
-
L-3-Aldonic acid dehydrogenase ...
Schwanniomyces occidentalis
Acta Biochim. Pol.
9
269-277
1964
-
-
-
-
-
-
3
4
-
-
-
1
-
1
-
-
1
-
-
-
-
-
5
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
4
-
-
-
1
-
-
-
1
-
-
-
-
5
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
286905
Smiley
-
Purification and properties of ...
Sus scrofa
J. Biol. Chem.
236
357-364
1961
1
-
-
-
-
-
2
8
1
1
-
1
-
1
-
-
1
-
-
1
-
1
16
-
-
-
-
-
2
2
-
2
-
-
-
1
-
-
2
-
-
-
-
2
-
8
1
1
-
1
-
-
-
1
-
1
-
1
16
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-