BRENDA - Enzyme Database show
show all sequences of 1.1.1.45

Purification and properties of beta-L-hydroxy acid dehydrogenase

Smiley, J.D.; Ashwell, G.; J. Biol. Chem. 236, 357-364 (1961)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
cysteine
in mM-concentration, no activation of the reverse reaction
Sus scrofa
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
not inhibitory iodoacetate, ethylendiamine tetraacetate, 2,2'-bipyridine, 8-hydroxyquiniline, O-phenanthroline, diethyldithiocarbamate, thiamine pyrophosphate
Sus scrofa
p-chloromercuribenzoate
in both directions
Sus scrofa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic studies
Sus scrofa
0.04
-
NAD+
cosubstrate L-gulonate, pH 8.5
Sus scrofa
0.45
-
beta-keto-L-gulonate
cofactor NADH, pH 6.3
Sus scrofa
5.1
-
L-gulonate
cofactor NAD+, pH 8.5
Sus scrofa
5.3
-
L-3-hydroxybutanoate
-
Sus scrofa
5.3
-
L-Idonate
cofactor NAD+, pH 8.5
Sus scrofa
6.4
-
acetoacetate
cofactor NADH, pH 6.3
Sus scrofa
7.3
-
2,3-diketo-L-gulonate
cofactor NADH, pH 6.3
Sus scrofa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Sus scrofa
5829
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no activation or inhibition Zn2+, Cd2+, Cu2+, Fe3+, Mn2+, Ni2+, Co3+, As3+
Sus scrofa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-gulonic acid + NAD+
Sus scrofa
glucuronate-xylulose cycle
3-dehydro-L-gulonic acid + NADH + H+
-
Sus scrofa
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sus scrofa
-
hog
-
Purification (Commentary)
Commentary
Organism
-
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Sus scrofa
-
Storage Stability
Storage Stability
Organism
-18C, 0.05 M K2HPO4, several weeks, little loss of activity
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-keto-L-gulonate + NADH
-
286905
Sus scrofa
2,3-diketo-L-gulonate + NAD+
-
-
-
ir
D-galactonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
D-gluconic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
D-lyxonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
D-mannonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
D-talonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
D-xylonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
L-3-hydroxybutanoate + NADH
-
286905
Sus scrofa
acetoacetate + NAD+
-
-
-
r
L-altronic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
L-erythronic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
L-gulonic acid + NAD+
stereospecificity for L-configuration of the beta-carbon hydroxyl group
286905
Sus scrofa
3-dehydro-L-gulonic acid + NADH + H+
-
-
-
r
L-gulonic acid + NAD+
glucuronate-xylulose cycle
286905
Sus scrofa
3-dehydro-L-gulonic acid + NADH + H+
-
286905
Sus scrofa
r
L-idonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
L-ribonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
L-threonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
additional information
no substrates are: L-arabonic acid, D-glucuronate, D-mannuronate, l-iduronate, 6-phospho-D-gluconate, L-serine, L-threonine, beta-D-hydroxy acids, acetoacetyl-CoA, acetoacetyl-S-pantetheine
286905
Sus scrofa
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3
-
reduction of 3-dehydro-L-gulonic acid
Sus scrofa
8.5
-
-
Sus scrofa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
8
reduction of acetoacetate
Sus scrofa
6.2
10
oxidation of L-3-hydroxybutanoate
Sus scrofa
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Sus scrofa
NADH
-
Sus scrofa
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
cysteine
in mM-concentration, no activation of the reverse reaction
Sus scrofa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Sus scrofa
NADH
-
Sus scrofa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
not inhibitory iodoacetate, ethylendiamine tetraacetate, 2,2'-bipyridine, 8-hydroxyquiniline, O-phenanthroline, diethyldithiocarbamate, thiamine pyrophosphate
Sus scrofa
p-chloromercuribenzoate
in both directions
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic studies
Sus scrofa
0.04
-
NAD+
cosubstrate L-gulonate, pH 8.5
Sus scrofa
0.45
-
beta-keto-L-gulonate
cofactor NADH, pH 6.3
Sus scrofa
5.1
-
L-gulonate
cofactor NAD+, pH 8.5
Sus scrofa
5.3
-
L-3-hydroxybutanoate
-
Sus scrofa
5.3
-
L-Idonate
cofactor NAD+, pH 8.5
Sus scrofa
6.4
-
acetoacetate
cofactor NADH, pH 6.3
Sus scrofa
7.3
-
2,3-diketo-L-gulonate
cofactor NADH, pH 6.3
Sus scrofa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Sus scrofa
5829
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no activation or inhibition Zn2+, Cd2+, Cu2+, Fe3+, Mn2+, Ni2+, Co3+, As3+
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-gulonic acid + NAD+
Sus scrofa
glucuronate-xylulose cycle
3-dehydro-L-gulonic acid + NADH + H+
-
Sus scrofa
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Sus scrofa
-
Storage Stability (protein specific)
Storage Stability
Organism
-18C, 0.05 M K2HPO4, several weeks, little loss of activity
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-keto-L-gulonate + NADH
-
286905
Sus scrofa
2,3-diketo-L-gulonate + NAD+
-
-
-
ir
D-galactonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
D-gluconic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
D-lyxonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
D-mannonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
D-talonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
D-xylonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
L-3-hydroxybutanoate + NADH
-
286905
Sus scrofa
acetoacetate + NAD+
-
-
-
r
L-altronic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
L-erythronic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
L-gulonic acid + NAD+
stereospecificity for L-configuration of the beta-carbon hydroxyl group
286905
Sus scrofa
3-dehydro-L-gulonic acid + NADH + H+
-
-
-
r
L-gulonic acid + NAD+
glucuronate-xylulose cycle
286905
Sus scrofa
3-dehydro-L-gulonic acid + NADH + H+
-
286905
Sus scrofa
r
L-idonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
L-ribonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
L-threonic acid + NAD+
-
286905
Sus scrofa
?
-
-
-
-
additional information
no substrates are: L-arabonic acid, D-glucuronate, D-mannuronate, l-iduronate, 6-phospho-D-gluconate, L-serine, L-threonine, beta-D-hydroxy acids, acetoacetyl-CoA, acetoacetyl-S-pantetheine
286905
Sus scrofa
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3
-
reduction of 3-dehydro-L-gulonic acid
Sus scrofa
8.5
-
-
Sus scrofa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
8
reduction of acetoacetate
Sus scrofa
6.2
10
oxidation of L-3-hydroxybutanoate
Sus scrofa
Other publictions for EC 1.1.1.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712765
Asada
Dimeric crystal structure of r ...
Oryctolagus cuniculus
J. Mol. Biol.
401
906-920
2010
-
-
1
1
4
-
-
17
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1
1
-
2
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1
1
-
2
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4
2
1
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1
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4
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1
4
1
4
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17
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1
1
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1
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2
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4
2
1
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1
-
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1
1
-
-
-
695345
Asada
Crystallization and preliminar ...
Oryctolagus cuniculus
Acta Crystallogr. Sect. F
64
228-230
2008
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-
1
1
-
-
-
-
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1
-
-
2
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1
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1
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1
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1
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1
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1
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1
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-
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-
669147
Ishikura
Structural and functional char ...
Oryctolagus cuniculus
J. Biochem.
137
303-314
2005
-
-
1
-
6
-
5
19
1
-
3
-
-
3
-
-
1
-
-
2
2
-
5
1
-
-
-
-
1
-
-
2
-
1
2
-
-
1
2
-
6
-
2
5
-
19
1
-
3
-
-
-
-
1
-
2
2
-
5
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
286146
Goode
Accumulation of xylitol in the ...
Bos taurus, Rattus norvegicus
FEBS Lett.
395
174-178
1996
-
2
-
-
-
-
-
-
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-
2
2
-
2
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-
2
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4
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4
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2
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4
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2
2
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2
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4
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286911
Menotti-Raymond
Further characterization of L- ...
Drosophila melanogaster
Biochim. Biophys. Acta
841
15-21
1985
-
-
-
-
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-
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1
1
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3
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1
1
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2
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1
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2
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2
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1
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1
1
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2
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2
1
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286910
Cannistraro
Subunit structure and kinetic ...
Drosophila melanogaster
Biochim. Biophys. Acta
569
1-5
1979
-
-
-
-
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1
2
4
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3
1
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2
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1
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3
1
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2
1
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3
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3
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1
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2
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4
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3
1
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1
-
3
1
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2
1
-
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-
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-
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-
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286909
Borack
-
Organ distribution of Drosophi ...
Drosophila melanogaster
Experientia
30
31
1974
-
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-
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1
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1
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4
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2
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2
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2
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1
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4
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2
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286908
Koundakjian
3-Hydroxy acid dehydrogenases ...
Ovis aries
Biochem. J.
127
449-452
1972
-
-
-
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1
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1
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286907
Borack
Drosophila beta-L-hydroxy acid ...
Drosophila melanogaster
J. Biol. Chem.
246
5345-5350
1970
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3
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1
1
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1
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4
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1
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286906
Dworsky
-
L-3-Aldonic acid dehydrogenase ...
Schwanniomyces occidentalis
Acta Biochim. Pol.
9
269-277
1964
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3
4
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5
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1
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1
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-
286905
Smiley
-
Purification and properties of ...
Sus scrofa
J. Biol. Chem.
236
357-364
1961
1
-
-
-
-
-
2
8
1
1
-
1
-
1
-
-
1
-
-
1
-
1
16
-
-
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-
-
2
2
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2
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1
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2
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2
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8
1
1
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1
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1
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1
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1
16
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2
2
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