Cloned (Comment) | Organism |
---|---|
gene idh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) | Zymomonas mobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Zymomonas mobilis | |
Co2+ | - |
Zymomonas mobilis | |
Cu2+ | complete inhibition | Zymomonas mobilis | |
Ni2+ | - |
Zymomonas mobilis | |
Zn2+ | complete inhibition | Zymomonas mobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.245 | - |
NAD+ | pH 7.5, 37°C, with Mn2+, recombinant enzyme | Zymomonas mobilis | |
0.312 | - |
NAD+ | pH 7.5, 37°C, with Mg2+, recombinant enzyme | Zymomonas mobilis | |
7.7 | - |
NADP+ | pH 7.5, 37°C, with Mn2+, recombinant enzyme | Zymomonas mobilis | |
8.2 | - |
NADP+ | pH 7.5, 37°C, with Mg2+, recombinant enzyme | Zymomonas mobilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates, 42.5% activity compared to Mn2+ | Zymomonas mobilis | |
Mg2+ | activates, 78% activity compared to Mn2+ | Zymomonas mobilis | |
Mn2+ | activates, best cation | Zymomonas mobilis | |
additional information | the recombinant ZmIDH activity is completely dependent on the divalent cation and Mn2+ is the most effective cation | Zymomonas mobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2 * 46000, recombinant His6-tagged enzyme, SDS-PAGE | Zymomonas mobilis |
74000 | - |
recombinant His6-tagged enzyme, gel filtration | Zymomonas mobilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | Zymomonas mobilis | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | Zymomonas mobilis ATCC 10988 | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | - |
ssp. mobiliss | - |
Zymomonas mobilis ATCC 10988 | - |
ssp. mobiliss | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) | Zymomonas mobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | - |
Zymomonas mobilis | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | poor performance of the recombinant ZmIDH in decarboxylation | Zymomonas mobilis | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | - |
Zymomonas mobilis ATCC 10988 | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | poor performance of the recombinant ZmIDH in decarboxylation | Zymomonas mobilis ATCC 10988 | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NADP+ | poor performance of the recombinant ZmIDH in decarboxylation | Zymomonas mobilis | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | poor performance of the recombinant ZmIDH in decarboxylation | Zymomonas mobilis ATCC 10988 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 46000, recombinant His6-tagged enzyme, SDS-PAGE | Zymomonas mobilis |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Zymomonas mobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Zymomonas mobilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | and above, activity range, profile overview | Zymomonas mobilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant His-tagged enzyme, rapid inactivation above | Zymomonas mobilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
NADP+ | pH 7.5, 37°C, with Mg2+, recombinant enzyme | Zymomonas mobilis | |
25 | - |
NADP+ | pH 7.5, 37°C, with Mn2+, recombinant enzyme | Zymomonas mobilis | |
88 | - |
NAD+ | pH 7.5, 37°C, with Mg2+, recombinant enzyme | Zymomonas mobilis | |
112 | - |
NAD+ | pH 7.5, 37°C, with Mn2+, recombinant enzyme | Zymomonas mobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
with Mn2+ | Zymomonas mobilis |
8.5 | - |
with Mg2+ | Zymomonas mobilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | activity range, profile overview | Zymomonas mobilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | ZmIDH displays a 165fold (kcat/Km) preference for NAD+ over NADP+ with Mg2+, and 142fold with Mn2+ | Zymomonas mobilis | |
NADP+ | ZmIDH displays a 165fold (kcat/Km) preference for NAD+ over NADP+ with Mg2+, and 142fold with Mn2+ | Zymomonas mobilis |
General Information | Comment | Organism |
---|---|---|
evolution | the recombinant ZmIDH is mainly NAD+-dependent and its catalytic efficiency (kcat/Km) is relative low when compared to the prokaryotic NADP+-IDHs | Zymomonas mobilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000002 | - |
NADP+ | pH 7.5, 37°C, with Mg2+, recombinant enzyme | Zymomonas mobilis | |
0.000003 | - |
NADP+ | pH 7.5, 37°C, with Mn2+, recombinant enzyme | Zymomonas mobilis | |
0.0003 | - |
NAD+ | pH 7.5, 37°C, with Mg2+, recombinant enzyme | Zymomonas mobilis | |
0.0005 | - |
NAD+ | pH 7.5, 37°C, with Mn2+, recombinant enzyme | Zymomonas mobilis |