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Literature summary for 1.1.1.41 extracted from
- Biochemical and molecular characterization of NAD(+)-dependent isocitrate dehydrogenase from the ethanologenic bacterium Zymomonas mobilis (2012), FEMS Microbiol. Lett., 327, 134-141.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene idh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) | Zymomonas mobilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Zymomonas mobilis |  |
| Co2+ | - |
Zymomonas mobilis | |
| Cu2+ | complete inhibition | Zymomonas mobilis | |
| Ni2+ | - |
Zymomonas mobilis | |
| Zn2+ | complete inhibition | Zymomonas mobilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.245 | - |
NAD+ | pH 7.5, 37°C, with Mn2+, recombinant enzyme | Zymomonas mobilis | |
| 0.312 | - |
NAD+ | pH 7.5, 37°C, with Mg2+, recombinant enzyme | Zymomonas mobilis | |
| 7.7 | - |
NADP+ | pH 7.5, 37°C, with Mn2+, recombinant enzyme | Zymomonas mobilis | |
| 8.2 | - |
NADP+ | pH 7.5, 37°C, with Mg2+, recombinant enzyme | Zymomonas mobilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates, 42.5% activity compared to Mn2+ | Zymomonas mobilis | |
| Mg2+ | activates, 78% activity compared to Mn2+ | Zymomonas mobilis | |
| Mn2+ | activates, best cation | Zymomonas mobilis | |
| additional information | the recombinant ZmIDH activity is completely dependent on the divalent cation and Mn2+ is the most effective cation | Zymomonas mobilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
2 * 46000, recombinant His6-tagged enzyme, SDS-PAGE | Zymomonas mobilis |
| 74000 | - |
recombinant His6-tagged enzyme, gel filtration | Zymomonas mobilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NAD+ | Zymomonas mobilis | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
| isocitrate + NAD+ | Zymomonas mobilis ATCC 10988 | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zymomonas mobilis | - |
ssp. mobiliss | - |
| Zymomonas mobilis ATCC 10988 | - |
ssp. mobiliss | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) | Zymomonas mobilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NAD+ | - |
Zymomonas mobilis | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
| isocitrate + NAD+ | poor performance of the recombinant ZmIDH in decarboxylation | Zymomonas mobilis | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
| isocitrate + NAD+ | - |
Zymomonas mobilis ATCC 10988 | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
| isocitrate + NAD+ | poor performance of the recombinant ZmIDH in decarboxylation | Zymomonas mobilis ATCC 10988 | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
| isocitrate + NADP+ | poor performance of the recombinant ZmIDH in decarboxylation | Zymomonas mobilis | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
| isocitrate + NADP+ | poor performance of the recombinant ZmIDH in decarboxylation | Zymomonas mobilis ATCC 10988 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 46000, recombinant His6-tagged enzyme, SDS-PAGE | Zymomonas mobilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IDH | - |
Zymomonas mobilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Zymomonas mobilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | and above, activity range, profile overview | Zymomonas mobilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant His-tagged enzyme, rapid inactivation above | Zymomonas mobilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14 | - |
NADP+ | pH 7.5, 37°C, with Mg2+, recombinant enzyme | Zymomonas mobilis | |
| 25 | - |
NADP+ | pH 7.5, 37°C, with Mn2+, recombinant enzyme | Zymomonas mobilis | |
| 88 | - |
NAD+ | pH 7.5, 37°C, with Mg2+, recombinant enzyme | Zymomonas mobilis | |
| 112 | - |
NAD+ | pH 7.5, 37°C, with Mn2+, recombinant enzyme | Zymomonas mobilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
with Mn2+ | Zymomonas mobilis |
| 8.5 | - |
with Mg2+ | Zymomonas mobilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | activity range, profile overview | Zymomonas mobilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | ZmIDH displays a 165fold (kcat/Km) preference for NAD+ over NADP+ with Mg2+, and 142fold with Mn2+ | Zymomonas mobilis | |
| NADP+ | ZmIDH displays a 165fold (kcat/Km) preference for NAD+ over NADP+ with Mg2+, and 142fold with Mn2+ | Zymomonas mobilis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the recombinant ZmIDH is mainly NAD+-dependent and its catalytic efficiency (kcat/Km) is relative low when compared to the prokaryotic NADP+-IDHs | Zymomonas mobilis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000002 | - |
NADP+ | pH 7.5, 37°C, with Mg2+, recombinant enzyme | Zymomonas mobilis | |
| 0.000003 | - |
NADP+ | pH 7.5, 37°C, with Mn2+, recombinant enzyme | Zymomonas mobilis | |
| 0.0003 | - |
NAD+ | pH 7.5, 37°C, with Mg2+, recombinant enzyme | Zymomonas mobilis | |
| 0.0005 | - |
NAD+ | pH 7.5, 37°C, with Mn2+, recombinant enzyme | Zymomonas mobilis | |
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