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Literature summary for 1.1.1.41 extracted from

  • Garcia, J.A.; Minard, K.I.; Lin, A.P.; McAlister-Henn, L.
    Disulfide bond formation in yeast NAD+-specific isocitrate dehydrogenase (2009), Biochemistry, 48, 8869-8878.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Gold (DE3) cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C150S insensitive to treatment with diamide, the presence of the IDH2 Cys-150 residue (and perhaps the potential for disulfide bond formation) may contribute to cooperativity and may also limit maximal IDH activity Saccharomyces cerevisiae
C56S/C242S shows activity similar to wild type enzyme and is sensitive to treatment with diamide Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Diamide treatment of the affinity-purified enzyme with diamide results in the formation of disulfide bonds and in decreased activity for the wild type enzyme, the effect is reversible by the addition of dithiothreitol Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
subunit IDH1, nonreducing PAGE Saccharomyces cerevisiae
123000
-
disulfide bonded form of subunit IDH2, nonreducing PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae MMY011
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-isocitrate + NAD+
-
Saccharomyces cerevisiae 2-oxoglutarate + CO2 + NADH
-
?
D-isocitrate + NAD+
-
Saccharomyces cerevisiae MMY011 2-oxoglutarate + CO2 + NADH
-
?

Subunits

Subunits Comment Organism
octamer the octameric enzyme is composed of four heterodimers of regulatory IDH1 and catalytic IDH2 subunits Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
IDH
-
Saccharomyces cerevisiae
NAD+-specific isocitrate dehydrogenase
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharomyces cerevisiae