Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Gold (DE3) cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C150S | insensitive to treatment with diamide, the presence of the IDH2 Cys-150 residue (and perhaps the potential for disulfide bond formation) may contribute to cooperativity and may also limit maximal IDH activity | Saccharomyces cerevisiae |
C56S/C242S | shows activity similar to wild type enzyme and is sensitive to treatment with diamide | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Diamide | treatment of the affinity-purified enzyme with diamide results in the formation of disulfide bonds and in decreased activity for the wild type enzyme, the effect is reversible by the addition of dithiothreitol | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
subunit IDH1, nonreducing PAGE | Saccharomyces cerevisiae |
123000 | - |
disulfide bonded form of subunit IDH2, nonreducing PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae MMY011 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-isocitrate + NAD+ | - |
Saccharomyces cerevisiae | 2-oxoglutarate + CO2 + NADH | - |
? | |
D-isocitrate + NAD+ | - |
Saccharomyces cerevisiae MMY011 | 2-oxoglutarate + CO2 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | the octameric enzyme is composed of four heterodimers of regulatory IDH1 and catalytic IDH2 subunits | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Saccharomyces cerevisiae |
NAD+-specific isocitrate dehydrogenase | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae |