Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Yarrowia lipolytica |
General Stability | Organism |
---|---|
20% glycerol stabilizes during storage | Yarrowia lipolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glyoxylate | slight inhibition | Yarrowia lipolytica | |
NADH | complete inhibition at 0.2 mM, competitive | Yarrowia lipolytica | |
oxaloacetate | slight inhibition | Yarrowia lipolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.136 | - |
NAD+ | pH 7.4, 25°C | Yarrowia lipolytica | |
0.581 | - |
isocitrate | pH 7.4, 25°C | Yarrowia lipolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Yarrowia lipolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
8 * 52000, SDS-PAGE | Yarrowia lipolytica |
412000 | - |
gel filtration | Yarrowia lipolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | Yarrowia lipolytica | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
additional information | Yarrowia lipolytica | enzyme is involved in tricarboxylic acid cycle and plays a key role in the regulation of biosynthesis of citrate and isocitrate, yeast is overproducing citrate under nitrogen deficiency in presence of glucose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yarrowia lipolytica | - |
citrate-overproducing yeast, strain BKM Y-2373 | - |
Purification (Comment) | Organism |
---|---|
129fold to homogeneity by ammonium sulfate fractionation, hydrophobic chromatography on an octyl resin, and gel filtration | Yarrowia lipolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.15 | - |
enzyme extract of cells in exponential growth phase | Yarrowia lipolytica |
0.16 | - |
enzyme extract of cells in stationary growth phase | Yarrowia lipolytica |
0.17 | - |
enzyme extract of cells in growth phase with acid synthesis | Yarrowia lipolytica |
22 | - |
purified enzyme | Yarrowia lipolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | - |
Yarrowia lipolytica | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
additional information | enzyme is involved in tricarboxylic acid cycle and plays a key role in the regulation of biosynthesis of citrate and isocitrate, yeast is overproducing citrate under nitrogen deficiency in presence of glucose | Yarrowia lipolytica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 52000, SDS-PAGE | Yarrowia lipolytica |
Synonyms | Comment | Organism |
---|---|---|
NAD+-dependent isocitrate dehydrogenase | - |
Yarrowia lipolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Yarrowia lipolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Yarrowia lipolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Yarrowia lipolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
NADH | pH 7.4, 25°C | Yarrowia lipolytica |