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show all sequences of 1.1.1.40

Comparative studies on the biochemical properties of the malic enzymes from Trypanosoma cruzi and Trypanosoma brucei

Leroux, A.; Maugeri, D.; Opperdoes, F.; Cazzulo, J.; Nowicki, C.; FEMS Microbiol. Lett. 314, 25-33 (2011)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
L-aspartate
the cytosolic isozyme is 50% activated by L-aspartate
Trypanosoma brucei
L-aspartate
10fold activation; the cytosolic isozyme is over 10fold activated by L-aspartate
Trypanosoma cruzi
Cloned(Commentary)
Commentary
Organism
cytosolic and mitochondrial isozymes, expression in of His-tagged isozymes ME1 and ME2 Escherichia coli Rosetta (DE3)pLysS; expression of His-tagged isozymes ME1 and ME2 in Escherichia coli strain BL21(DE3)
Trypanosoma brucei
cytosolic and mitochondrial isozymes, expression of His-tagged ME2 in Escherichia coli Rosetta (DE3)pLysS and of His-tagged ME1 in Escherichia coli BL(DE3); expression of His-tagged isozymes ME1 and ME2 in Escherichia coli strain BL21(DE3)
Trypanosoma cruzi
Crystallization (Commentary)
Crystallization
Organism
recombinant His-tagged cytosolic and mitochondrial isozymes from Escherichia coli by nickel affinity chromatography
Trypanosoma brucei
Inhibitors
Inhibitors
Commentary
Organism
Structure
oxaloacetate
competitive
Trypanosoma brucei
oxaloacetate
competitive
Trypanosoma cruzi
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
NADP+
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
0.027
-
NADP+
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
0.032
-
NADP+
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
0.048
-
NADP+
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
0.13
-
(S)-malate
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
0.32
-
(S)-malate
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
0.49
-
(S)-malate
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
2.3
-
(S)-malate
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
4.3
-
pyruvate
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
5.1
-
pyruvate
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
5.3
-
pyruvate
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
7.6
-
pyruvate
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
both cytosolic and mitochondrial isozymes are more abundant in the insect stage, although they can be immunodetected in the bloodstream forms; cytosolic isozyme, appears to be clearly more abundant in the insect stage, although it can be immunodetected in the bloodstream form
Trypanosoma brucei
5829
-
cytosol
cytosolic isozyme, the cytosolic isoform appears to be more abundant in the insect stages of the parasite; the cytosolic isoform is more abundant in the insect stages of the parasite
Trypanosoma cruzi
5829
-
mitochondrion
both cytosolic and mitochondrial isozymes are more abundant in the insect stage, although they can be immunodetected in the bloodstream forms; mitochondrial isozyme, appears to be clearly more abundant in the insect stage, although it can be immunodetected in the bloodstream form
Trypanosoma brucei
5739
-
mitochondrion
expression of the mitochondrial ME is upregulated in amastigotes; mitochondrial isozyme, the expression of the mitochondrial isozyme seems to be clearly upregulated in amastigotes
Trypanosoma cruzi
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
activates, kcat is 1280/s and Km 0.27 mM for ME1, and kcat is 208/s and Km 0.58 mM for ME2; can partially substitute for Mn2+, activates
Trypanosoma brucei
Mg2+
can partially substitute for Mn2+, activates; highly activating, kcat is 461/s and Km 0.04 mM for ME1, and kcat is 864/s and Km 0.57 mM for ME2
Trypanosoma cruzi
Mn2+
activates, required; highly activating, kcat is 905/s and Km 0.0024 mM for ME1, and kcat is 246/s and Km 0.0066 mM for ME2
Trypanosoma brucei
Mn2+
activates, kcat is 518/s and Km 0.0043 mM for ME1, and kcat is 687/s and Km 0.0091 mM for ME2; activates, required
Trypanosoma cruzi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-malate + NADP+
Trypanosoma brucei
-
pyruvate + CO2 + NADPH
-
-
r
(S)-malate + NADP+
Trypanosoma cruzi
-
pyruvate + CO2 + NADPH
-
-
r
(S)-malate + NADP+
Trypanosoma brucei
-
pyruvate + NADPH + H+ + CO2
-
-
r
(S)-malate + NADP+
Trypanosoma cruzi
-
pyruvate + NADPH + H+ + CO2
-
-
?
(S)-malate + NADP+
Trypanosoma cruzi CL Brener
-
pyruvate + CO2 + NADPH
-
-
r
(S)-malate + NADP+
Trypanosoma cruzi CL Brener
-
pyruvate + NADPH + H+ + CO2
-
-
?
(S)-malate + NADP+
Trypanosoma brucei 427
-
pyruvate + CO2 + NADPH
-
-
r
(S)-malate + NADP+
Trypanosoma brucei 427
-
pyruvate + NADPH + H+ + CO2
-
-
r
(S)-malate + NADP+
Trypanosoma brucei stock 427
-
pyruvate + CO2 + NADPH
-
-
r
(S)-malate + NADP+
Trypanosoma brucei stock 427
-
pyruvate + NADPH + H+ + CO2
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trypanosoma brucei
-
; grown in male Wistar rats
-
Trypanosoma brucei 427
-
-
-
Trypanosoma brucei stock 427
-
grown in male Wistar rats
-
Trypanosoma cruzi
-
-
-
Trypanosoma cruzi CL Brener
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged cytosolic and mitochondrial isozymes from Escherichia coli by nickel affinity chromatography; recombinant His-tagged isozymes ME1 and ME2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Trypanosoma cruzi
recombinant His-tagged isozymes ME1 and ME2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Trypanosoma brucei
Source Tissue
Source Tissue
Commentary
Organism
Textmining
amastigote
-
Trypanosoma cruzi
-
epimastigote
-
Trypanosoma cruzi
-
additional information
both cytosolic and mitochondrial isozymes are more abundant in the insect stage, although they can be immunodetected in the bloodstream forms; both isozymes appear to be clearly more abundant in the insect stage, although they can be immunodetected in the bloodstream forms
Trypanosoma brucei
-
additional information
expression of the mitochondrial ME is upregulated in amastigotes, while the cytosolic isoform is more abundant in the insect stages of the parasite; the expression of the mitochondrial isozyme seems to be clearly upregulated in amastigotes, whereas the cytosolic isoform appears to be more abundant in the insect stages of the parasite
Trypanosoma cruzi
-
procyclic form
-
Trypanosoma brucei
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-malate + NADP+
-
712042
Trypanosoma brucei
pyruvate + CO2 + NADPH
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma cruzi
pyruvate + CO2 + NADPH
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma cruzi CL Brener
pyruvate + CO2 + NADPH
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma brucei 427
pyruvate + CO2 + NADPH
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma brucei stock 427
pyruvate + CO2 + NADPH
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma brucei
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma cruzi
pyruvate + NADPH + H+ + CO2
-
-
-
?
(S)-malate + NADP+
oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs
712042
Trypanosoma brucei
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs
712042
Trypanosoma cruzi
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma cruzi CL Brener
pyruvate + NADPH + H+ + CO2
-
-
-
?
(S)-malate + NADP+
oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs
712042
Trypanosoma cruzi CL Brener
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma brucei 427
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs
712042
Trypanosoma brucei 427
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma brucei stock 427
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs
712042
Trypanosoma brucei stock 427
pyruvate + NADPH + H+ + CO2
-
-
-
r
Subunits
Subunits
Commentary
Organism
tetramer
-
Trypanosoma brucei
tetramer
-
Trypanosoma cruzi
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.3
-
pyruvate
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
34
-
pyruvate
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
39
-
pyruvate
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
113
-
pyruvate
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
242
-
(S)-malate
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
284
-
NADP+
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
504
-
NADP+
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
518
-
(S)-malate
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
683
-
(S)-malate
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
691
-
NADP+
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
1132
-
(S)-malate
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
1206
-
NADP+
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Trypanosoma brucei
7.4
-
assay at
Trypanosoma cruzi
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
; specific for
Trypanosoma brucei
NADP+
; specific for
Trypanosoma cruzi
NADPH
; specific for
Trypanosoma brucei
NADPH
; specific for
Trypanosoma cruzi
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
L-aspartate
the cytosolic isozyme is 50% activated by L-aspartate
Trypanosoma brucei
L-aspartate
10fold activation; the cytosolic isozyme is over 10fold activated by L-aspartate
Trypanosoma cruzi
Cloned(Commentary) (protein specific)
Commentary
Organism
cytosolic and mitochondrial isozymes, expression in of His-tagged isozymes ME1 and ME2 Escherichia coli Rosetta (DE3)pLysS; expression of His-tagged isozymes ME1 and ME2 in Escherichia coli strain BL21(DE3)
Trypanosoma brucei
cytosolic and mitochondrial isozymes, expression of His-tagged ME2 in Escherichia coli Rosetta (DE3)pLysS and of His-tagged ME1 in Escherichia coli BL(DE3); expression of His-tagged isozymes ME1 and ME2 in Escherichia coli strain BL21(DE3)
Trypanosoma cruzi
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
; specific for
Trypanosoma brucei
NADP+
; specific for
Trypanosoma cruzi
NADPH
; specific for
Trypanosoma brucei
NADPH
; specific for
Trypanosoma cruzi
Crystallization (Commentary) (protein specific)
Crystallization
Organism
recombinant His-tagged cytosolic and mitochondrial isozymes from Escherichia coli by nickel affinity chromatography
Trypanosoma brucei
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
oxaloacetate
competitive
Trypanosoma brucei
oxaloacetate
competitive
Trypanosoma cruzi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
NADP+
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
0.027
-
NADP+
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
0.032
-
NADP+
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
0.048
-
NADP+
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
0.13
-
(S)-malate
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
0.32
-
(S)-malate
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
0.49
-
(S)-malate
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
2.3
-
(S)-malate
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
4.3
-
pyruvate
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
5.1
-
pyruvate
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
5.3
-
pyruvate
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
7.6
-
pyruvate
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
both cytosolic and mitochondrial isozymes are more abundant in the insect stage, although they can be immunodetected in the bloodstream forms; cytosolic isozyme, appears to be clearly more abundant in the insect stage, although it can be immunodetected in the bloodstream form
Trypanosoma brucei
5829
-
cytosol
cytosolic isozyme, the cytosolic isoform appears to be more abundant in the insect stages of the parasite; the cytosolic isoform is more abundant in the insect stages of the parasite
Trypanosoma cruzi
5829
-
mitochondrion
both cytosolic and mitochondrial isozymes are more abundant in the insect stage, although they can be immunodetected in the bloodstream forms; mitochondrial isozyme, appears to be clearly more abundant in the insect stage, although it can be immunodetected in the bloodstream form
Trypanosoma brucei
5739
-
mitochondrion
expression of the mitochondrial ME is upregulated in amastigotes; mitochondrial isozyme, the expression of the mitochondrial isozyme seems to be clearly upregulated in amastigotes
Trypanosoma cruzi
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
activates, kcat is 1280/s and Km 0.27 mM for ME1, and kcat is 208/s and Km 0.58 mM for ME2; can partially substitute for Mn2+, activates
Trypanosoma brucei
Mg2+
can partially substitute for Mn2+, activates; highly activating, kcat is 461/s and Km 0.04 mM for ME1, and kcat is 864/s and Km 0.57 mM for ME2
Trypanosoma cruzi
Mn2+
activates, required; highly activating, kcat is 905/s and Km 0.0024 mM for ME1, and kcat is 246/s and Km 0.0066 mM for ME2
Trypanosoma brucei
Mn2+
activates, kcat is 518/s and Km 0.0043 mM for ME1, and kcat is 687/s and Km 0.0091 mM for ME2; activates, required
Trypanosoma cruzi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-malate + NADP+
Trypanosoma brucei
-
pyruvate + CO2 + NADPH
-
-
r
(S)-malate + NADP+
Trypanosoma cruzi
-
pyruvate + CO2 + NADPH
-
-
r
(S)-malate + NADP+
Trypanosoma brucei
-
pyruvate + NADPH + H+ + CO2
-
-
r
(S)-malate + NADP+
Trypanosoma cruzi
-
pyruvate + NADPH + H+ + CO2
-
-
?
(S)-malate + NADP+
Trypanosoma cruzi CL Brener
-
pyruvate + CO2 + NADPH
-
-
r
(S)-malate + NADP+
Trypanosoma cruzi CL Brener
-
pyruvate + NADPH + H+ + CO2
-
-
?
(S)-malate + NADP+
Trypanosoma brucei 427
-
pyruvate + CO2 + NADPH
-
-
r
(S)-malate + NADP+
Trypanosoma brucei 427
-
pyruvate + NADPH + H+ + CO2
-
-
r
(S)-malate + NADP+
Trypanosoma brucei stock 427
-
pyruvate + CO2 + NADPH
-
-
r
(S)-malate + NADP+
Trypanosoma brucei stock 427
-
pyruvate + NADPH + H+ + CO2
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged cytosolic and mitochondrial isozymes from Escherichia coli by nickel affinity chromatography; recombinant His-tagged isozymes ME1 and ME2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Trypanosoma cruzi
recombinant His-tagged isozymes ME1 and ME2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Trypanosoma brucei
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
amastigote
-
Trypanosoma cruzi
-
epimastigote
-
Trypanosoma cruzi
-
additional information
both cytosolic and mitochondrial isozymes are more abundant in the insect stage, although they can be immunodetected in the bloodstream forms; both isozymes appear to be clearly more abundant in the insect stage, although they can be immunodetected in the bloodstream forms
Trypanosoma brucei
-
additional information
expression of the mitochondrial ME is upregulated in amastigotes, while the cytosolic isoform is more abundant in the insect stages of the parasite; the expression of the mitochondrial isozyme seems to be clearly upregulated in amastigotes, whereas the cytosolic isoform appears to be more abundant in the insect stages of the parasite
Trypanosoma cruzi
-
procyclic form
-
Trypanosoma brucei
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-malate + NADP+
-
712042
Trypanosoma brucei
pyruvate + CO2 + NADPH
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma cruzi
pyruvate + CO2 + NADPH
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma cruzi CL Brener
pyruvate + CO2 + NADPH
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma brucei 427
pyruvate + CO2 + NADPH
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma brucei stock 427
pyruvate + CO2 + NADPH
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma brucei
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma cruzi
pyruvate + NADPH + H+ + CO2
-
-
-
?
(S)-malate + NADP+
oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs
712042
Trypanosoma brucei
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs
712042
Trypanosoma cruzi
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma cruzi CL Brener
pyruvate + NADPH + H+ + CO2
-
-
-
?
(S)-malate + NADP+
oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs
712042
Trypanosoma cruzi CL Brener
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma brucei 427
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs
712042
Trypanosoma brucei 427
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
-
712042
Trypanosoma brucei stock 427
pyruvate + NADPH + H+ + CO2
-
-
-
r
(S)-malate + NADP+
oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs
712042
Trypanosoma brucei stock 427
pyruvate + NADPH + H+ + CO2
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
-
Trypanosoma brucei
tetramer
-
Trypanosoma cruzi
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.3
-
pyruvate
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
34
-
pyruvate
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
39
-
pyruvate
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
113
-
pyruvate
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
242
-
(S)-malate
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
284
-
NADP+
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
504
-
NADP+
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
518
-
(S)-malate
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
683
-
(S)-malate
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
691
-
NADP+
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
1132
-
(S)-malate
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
1206
-
NADP+
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Trypanosoma brucei
7.4
-
assay at
Trypanosoma cruzi
General Information
General Information
Commentary
Organism
physiological function
in those environments where glucose is very low or absent, the pathogen depends on NADP-linked dehydrogenases such as the MEs for NADPH production, as in those conditions the pentose phosphate pathway cannot serve as a source of essential reducing power; possibly, in those environments where glucose is very low or absent, the pathogen depends on NADP-linked dehydrogenases such as the MEs for NADPH production, as in those conditions the pentose phosphate pathway cannot serve as a source of essential reducing power
Trypanosoma brucei
physiological function
in those environments where glucose is very low or absent, the pathogen depends on NADP-linked dehydrogenases such as the MEs for NADPH production, as in those conditions the pentose phosphate pathway cannot serve as a source of essential reducing power; possibly, in those environments where glucose is very low or absent, the pathogen depends on NADP-linked dehydrogenases such as the MEs for NADPH production, as in those conditions the pentose phosphate pathway cannot serve as a source of essential reducing power
Trypanosoma cruzi
General Information (protein specific)
General Information
Commentary
Organism
physiological function
in those environments where glucose is very low or absent, the pathogen depends on NADP-linked dehydrogenases such as the MEs for NADPH production, as in those conditions the pentose phosphate pathway cannot serve as a source of essential reducing power; possibly, in those environments where glucose is very low or absent, the pathogen depends on NADP-linked dehydrogenases such as the MEs for NADPH production, as in those conditions the pentose phosphate pathway cannot serve as a source of essential reducing power
Trypanosoma brucei
physiological function
in those environments where glucose is very low or absent, the pathogen depends on NADP-linked dehydrogenases such as the MEs for NADPH production, as in those conditions the pentose phosphate pathway cannot serve as a source of essential reducing power; possibly, in those environments where glucose is very low or absent, the pathogen depends on NADP-linked dehydrogenases such as the MEs for NADPH production, as in those conditions the pentose phosphate pathway cannot serve as a source of essential reducing power
Trypanosoma cruzi
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1
-
pyruvate
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
6
-
pyruvate
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
8
-
pyruvate
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
26
-
pyruvate
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
297
-
(S)-malate
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
494
-
(S)-malate
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
3536
-
(S)-malate
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
3981
-
(S)-malate
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
17780
-
NADP+
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
18680
-
NADP+
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
21600
-
NADP+
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
25170
-
NADP+
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1
-
pyruvate
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
6
-
pyruvate
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
8
-
pyruvate
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
26
-
pyruvate
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
297
-
(S)-malate
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
494
-
(S)-malate
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
3536
-
(S)-malate
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
3981
-
(S)-malate
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
17780
-
NADP+
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma brucei
18680
-
NADP+
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
21600
-
NADP+
isozyme ME2, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
Trypanosoma cruzi
25170
-
NADP+
isozyme ME1, pH 7.4, temperature not specified in the publication; pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
Trypanosoma brucei
Other publictions for EC 1.1.1.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738086
Nakajima Munekage
Light harvesting and chloropla ...
Zea mays
Curr. Opin. Plant Biol.
31
9-15
2016
-
-
-
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738993
Xue
The pivotal role of malic enzy ...
Phaeodactylum tricornutum
Microb. Cell Fact.
15
120
2016
-
-
1
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739074
Singh
Magnaporthe oryzae effector AV ...
Oryza sativa
Mol. Cells
39
426-438
2016
-
-
1
-
-
-
1
-
-
-
-
1
-
5
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
739138
Liu
Malic enzyme tracers reveal hy ...
Mus musculus
Nat. Chem. Biol.
12
345-352
2016
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737603
Kim
Overexpression of malic enzyme ...
Drosophila melanogaster
Biochem. Biophys. Res. Commun.
456
676-682
2015
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
739641
Liang
-
Characterization of malic enzy ...
Mortierella alpina, Mucoromycotina sp.
RSC Adv.
5
45558-45570
2015
-
-
-
-
-
-
-
-
2
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728207
Doubnerova Hyskova
Phosphoenolpyruvate carboxylas ...
Nicotiana tabacum
J. Plant Physiol.
171
19-25
2014
-
-
-
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
737473
Yang
Expression, purification, and ...
Mortierella alpina, Mortierella alpina ATCC32222
Appl. Biochem. Biotechnol.
173
1849-1857
2014
-
-
1
-
-
-
9
2
-
1
-
2
-
5
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
1
2
-
-
-
-
1
1
-
-
-
-
9
2
2
-
1
-
2
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
737500
Hao
Role of malic enzyme during fa ...
Mortierella alpina, Mortierella alpina ATCC 32222
Appl. Environ. Microbiol.
80
2672-2678
2014
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
737754
Hsieh
Structural characteristics of ...
Homo sapiens
Biochim. Biophys. Acta
1844
1773-1783
2014
1
-
1
1
11
-
-
18
2
1
-
1
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
9
-
-
-
1
-
-
-
1
-
1
1
1
11
-
-
-
-
18
2
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
9
-
-
-
-
-
-
-
-
-
-
739349
Wang
Elements required for an effic ...
Oryza sativa
Plant Physiol.
164
2231-2246
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722082
Niedzwiecka
Purification and properties of ...
Clupea harengus
Comp. Biochem. Physiol. B
164
216-220
2013
-
-
-
-
-
-
-
-
-
1
1
1
-
6
-
-
1
-
-
-
-
-
1
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
723150
Yu
Characterization of the NADP-m ...
Populus trichocarpa
Mol. Biol. Rep.
40
1385-1396
2013
2
-
1
-
-
-
4
11
2
-
-
1
-
5
-
-
1
-
-
3
-
-
1
1
1
-
-
10
1
-
-
1
-
-
-
2
-
1
1
-
-
-
-
4
-
11
2
-
-
1
-
-
-
1
-
3
-
-
1
1
1
-
-
10
1
-
-
-
2
1
1
2
10
10
723442
Arias
Fumarate and cytosolic pH as m ...
Arabidopsis thaliana
Plant Mol. Biol.
81
297-307
2013
2
-
1
-
1
-
4
1
1
1
-
1
-
4
-
-
-
-
-
-
-
-
1
-
1
-
-
-
2
-
-
2
-
-
-
2
-
1
2
-
1
-
-
4
-
1
1
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
737529
Rodriguez-Frometa
Malic enzyme activity is not t ...
Mucor circinelloides, Mucor circinelloides R7B
Appl. Microbiol. Biotechnol.
97
3063-3072
2013
-
-
1
-
-
-
-
-
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1
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3
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2
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699256
Cousins
C4 photosynthetic isotope exch ...
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7
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7
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7
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7
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7
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700772
Detarsio
Maize cytosolic NADP-malic enz ...
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1
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2
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3
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3
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1
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1
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1
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1
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3
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1
1
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1
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685622
Chang
Metal ions stabilize a dimeric ...
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2
2
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1
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2
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5
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2
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1
1
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1
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2
2
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1
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685731
Cheng
A cytosolic NADP-malic enzyme ...
Oryza sativa
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1
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2
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1
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1
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1
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2
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1
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1
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1
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3
2
-
1
-
-
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1
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-
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686932
Doubnerova
The activity and isoforms of N ...
Nicotiana benthamiana
Gen. Physiol. Biophys.
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1
1
5
1
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10
1
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2
3
1
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1
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2
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2
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1
1
5
1
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-
10
1
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2
3
1
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-
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1
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687132
Spampinato
Conformational changes of maiz ...
Triticum aestivum, Zea mays
Int. J. Biol. Macromol.
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2
8
2
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1
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2
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4
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4
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-
-
-
-
-
-
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2
2
2
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-
-
-
-
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2
8
2
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1
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2
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687342
Kwon
The effect of NADP-dependent m ...
Escherichia coli
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-
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2
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2
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1
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1
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2
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-
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1
2
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1
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-
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2
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1
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2
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2
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1
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687388
Bologna
Escherichia coli malic enzymes ...
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-
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2
1
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1
2
1
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2
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-
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1
2
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-
-
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1
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3
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2
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1
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1
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-
-
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2
1
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1
2
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687568
Pongratz
Cytosolic and mitochondrial ma ...
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3
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2
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2
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1
2
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1
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2
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3
1
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2
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687652
Guay
A role for ATP-citrate lyase, ...
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2
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2
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1
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2
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2
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1
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1
1
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1
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2
-
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2
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-
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1
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687668
Detarsio
Identification of domains invo ...
Zea mays
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1
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1
1
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2
1
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2
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1
2
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1
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3
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5
2
1
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1
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1
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1
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1
1
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2
1
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688314
Shin
5-aminolevulinic acid biosynth ...
Escherichia coli
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1
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1
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1
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2
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1
2
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2
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1
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1
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1
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Hameister
Transcriptional regulation of ...
Arabidopsis thaliana
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1
2
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1
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1
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688860
Zhang
Malic enzyme: the controlling ...
Mortierella alpina, Mucor circinelloides
Microbiology
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4
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4
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4
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2
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689392
Estavillo
Characterization of the NADP m ...
Hydrilla verticillata
Photosyn. Res.
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43-57
2007
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1
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1
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2
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3
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2
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3
6
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2
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3
3
4
3
3
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2
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3
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3
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689557
Liu
Expression of an NADP-malic en ...
Oryza sativa
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1
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2
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1
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3
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1
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-
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-
-
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689596
Fahnenstich
Alteration of organic acid met ...
Zea mays
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1
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1
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3
1
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2
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2
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1
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2
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2
2
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1
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1
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668573
Chang
Critical roles of conserved ca ...
Columba livia
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9
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1
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1
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4
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10
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2
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1
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1
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9
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Mitsch
Malic Enzyme Cofactor and Doma ...
Sinorhizobium meliloti
J. Bacteriol.
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1
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1
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2
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2
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1
2
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1
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-
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2
-
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-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670777
Cheng
Expression, purification, and ...
Oryza sativa
Protein Expr. Purif.
45
200-205
2006
-
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1
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-
-
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4
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-
-
-
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4
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1
-
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1
2
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2
-
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4
2
2
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1
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-
-
-
2
2
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-
-
-
-
-
4
-
-
-
-
-
-
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2
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1
2
-
2
-
-
-
-
4
2
2
-
-
-
-
-
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670499
Pinto
-
Site directed mutagenesis stud ...
Zea mays
Physiol. Mol. Biol. Plants
11
219-224
2005
-
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1
-
-
-
-
-
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1
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-
1
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-
-
-
-
-
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-
-
-
-
-
-
-
-
-
1
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1
1
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-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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670588
Ghannoum
Faster Rubisco is the key to s ...
Bothriochloa biloba, Bothriochloa biloba Kuntze, Bothriochloa bladhii, Bothriochloa bladhii Kuntze, Bothriochloa pertusa, Cenchrus ciliaris, Cenchrus clandestinus, Cenchrus compressus, Cymbopogon ambiguus, Cymbopogon ambiguus Spreng., Cymbopogon bombycinus, Cymbopogon bombycinus Spreng., Dichanthium aristatum, Dichanthium sericeum, Digitaria brownii, Digitaria smutsi, Paspalum dilatatum, Paspalum notatum, Sorghum bicolor
Plant Physiol.
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638-650
2005
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-
-
-
-
-
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-
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670594
Wheeler
A comprehensive analysis of th ...
Arabidopsis thaliana
Plant Physiol.
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39-51
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1
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1
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18
-
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1
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5
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-
-
1
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-
-
-
-
1
1
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-
-
-
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8
1
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-
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18
-
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1
-
-
-
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5
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-
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670917
Tsukatani
Sequential fluorometric quanti ...
Gallus gallus
Talanta
65
396-401
2005
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1
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-
-
-
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1
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-
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-
-
-
-
-
-
-
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-
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1
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-
-
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-
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-
-
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-
-
-
-
-
-
-
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-
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654369
Shearer
Characterization of NADP-depen ...
Ricinus communis
Arch. Biochem. Biophys.
429
134-144
2004
10
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9
2
1
2
2
1
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3
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1
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5
1
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2
1
1
-
1
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1
1
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2
6
-
-
10
-
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2
-
-
-
-
9
6
2
1
2
2
1
-
-
-
1
-
5
1
-
2
1
1
-
1
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1
1
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-
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654547
Detarsio
Basic residues play key roles ...
Zea mays
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1
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1
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-
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3
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1
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6
1
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2
4
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-
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1
2
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3
-
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2
4
10
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1
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-
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1
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-
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3
-
1
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6
1
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-
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655672
Dolezal
Malic enzymes of Trichomonas v ...
Trichomonas vaginalis, Trichomonas vaginalis TV 7-37
Gene
329
81-92
2004
1
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1
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1
2
2
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6
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1
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1
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8
1
1
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-
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1
-
-
2
-
1
-
1
-
1
2
-
-
-
-
1
-
2
2
2
2
4
-
-
-
1
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1
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8
1
1
-
-
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1
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1
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656120
Detarsio
Maize C4 NADP-malic enzyme. Ex ...
Zea mays
J. Biol. Chem.
278
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2003
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1
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4
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1
12
1
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6
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1
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1
-
-
3
2
-
-
-
7
1
-
-
2
1
1
-
-
-
1
2
-
4
-
-
1
1
12
1
1
3
1
-
-
-
1
-
1
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3
2
-
-
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7
1
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1
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656619
Murmu
Light activation of NADP malic ...
Zea mays
J. Plant Physiol.
160
51-56
2003
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1
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2
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2
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1
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1
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1
4
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3
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1
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-
-
-
3
4
2
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1
-
2
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-
-
-
-
2
-
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2
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1
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1
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657103
Ferreyra
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Purification and physical and ...
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2
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1
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1
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655613
Jernejc
The influence of metal ions on ...
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2002
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1
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655710
Pinto
Inactivation of maize NADP-mal ...
Zea mays
Indian J. Biochem. Biophys.
39
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2002
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656402
Laporte
Engineering for drought avoida ...
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2002
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1
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657017
Tausta
Maize C4 and non-C4 NADP-depen ...
Zea mays
Plant Mol. Biol.
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2002
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5
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1
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2
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2
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657035
Lai
Distinct but conserved functio ...
Flaveria angustifolia, Flaveria bidentis, Flaveria brownii, Flaveria floridana, Flaveria linearis, Flaveria pringlei, Flaveria robusta, Flaveria trinervia
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8
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3
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24
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16
-
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657292
Yang
Structural studies of the pige ...
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1
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1
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1
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286732
Drincovich
NADP-malic enzyme from plants: ...
Aloe arborescens, Apium graveolens, Egeria densa, Flaveria bidentis, Flaveria floridana, Haloxylon persicum, Mesembryanthemum crystallinum, Populus sp., Solanum lycopersicum, Triticum aestivum, Zea mays
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7
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6
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7
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286739
Gourdon
Cloning of the malic enzyme ge ...
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Appl. Environ. Microbiol.
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286706
Voegele
Characterization of two member ...
Sinorhizobium meliloti
Biochim. Biophys. Acta
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1999
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2
2
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286743
Fang
Cell-specific regulation of tr ...
Gallus gallus
Arch. Biochem. Biophys.
349
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Colombo
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Carbon metabolism in germinati ...
Ricinus communis
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4
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1
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2
1
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286734
Bukato
Different regulatory propertie ...
Homo sapiens
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Bukato
Purification and properties of ...
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1
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2
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286723
Hsu
Duck liver 'malic' enzyme. Exp ...
Anas platyrhynchos
Biochem. J.
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1992
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286740
Chang
Kinetic mechanism of the cytos ...
Homo sapiens
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Zelewski
Malic enzyme in human liver. I ...
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286741
Chang
Purification and characterizat ...
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1
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286748
Rouleau
-
Some properties of malic enzym ...
Pandalus borealis
Comp. Biochem. Physiol. B
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903-909
1991
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1
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Guagliardi
Stability and activity of a th ...
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2
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680589
Bartolucci
Malic enzyme from archaebacter ...
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1
3
1
1
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4
1
1
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2
1
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286737
Goodenough
-
NADP-linked malic enzyme and m ...
Solanum lycopersicum
Phytochemistry
24
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2
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5
2
1
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2
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1
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1
1
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286736
Vernon
Pigeon liver malic enzyme: inv ...
Pigeon
Arch. Biochem. Biophys.
225
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1983
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2
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1
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1
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286733
Dubery
-
Purification and characterizat ...
Mangifera indica
Biochim. Biophys. Acta
662
102-110
1981
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3
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1
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286731
Reynolds
Transient kinetic studies of m ...
Pigeon
Arch. Biochem. Biophys.
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1
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1
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286729
Jechova
Regulation of biosynthesis of ...
Streptomyces aureofaciens
Folia Microbiol. (Praha)
20
137-141
1975
-
-
-
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2
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2
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3
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1
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2
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2
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1
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286730
Wada
Crystallization and properties ...
Rattus norvegicus
Biochim. Biophys. Acta
410
237-242
1975
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1
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1
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286714
Mounib
NAD- and NADP-malic enzymes in ...
Bos taurus, Gadidae, Homo sapiens, salmon
FEBS Lett.
48
79-84
1974
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4
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286715
Lin
Malic enzymes of rabbit heart ...
Oryctolagus cuniculus
J. Biol. Chem.
249
3867-3875
1974
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3
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1
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286728
Ziegler
Malate dehydrogenase in Zea ma ...
Zea mays
Biochim. Biophys. Acta
364
28-37
1974
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4
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2
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-
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2
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3
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4
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1
2
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-
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1
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-
-
-
-
2
-
-
-
-
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286716
Eyzaguirre
Two malic enzymes in Pseudomon ...
Pseudomonas aeruginosa
J. Bacteriol.
116
215-221
1973
1
-
-
-
-
-
-
2
-
3
2
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-
1
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1
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-
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2
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-
-
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1
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1
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1
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-
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2
-
3
2
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1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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286727
Coombs
The C-4 pathway in Pennisetum ...
Cenchrus purpureus
Planta
110
109-120
1973
-
-
-
-
-
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1
2
-
1
-
-
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3
-
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1
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1
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1
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-
-
-
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1
1
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-
-
-
-
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-
-
-
-
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1
-
2
-
1
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
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-
286724
Clifford
Stereochemical course of decar ...
Gallus gallus
Eur. J. Biochem.
26
401-406
1972
-
-
-
-
-
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1
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1
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1
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1
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1
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-
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-
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1
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1
-
-
-
-
-
-
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286725
Bartholome
Purification and properties of ...
Sus scrofa
Hoppe-Seyler's Z. Physiol. Chem.
353
1487-1495
1972
-
-
-
-
-
-
3
6
2
2
2
-
-
1
-
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1
-
-
1
1
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2
-
-
-
-
-
3
-
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2
-
-
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-
-
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2
-
-
-
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3
-
6
2
2
2
-
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-
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1
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1
1
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2
-
-
-
-
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3
-
-
-
-
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286726
Nolte
Intracellular distribution of ...
Cavia porcellus, Locusta migratoria, Oryctolagus cuniculus, Pigeon, Rattus norvegicus, seagull
Biochim. Biophys. Acta
284
497-507
1972
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6
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13
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13
-
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286722
Rutter
Purification and properties of ...
Pigeon
J. Biol. Chem.
233
374-382
1958
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-
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2
10
4
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6
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1
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1
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1
1
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3
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1
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2
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2
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-
-
-
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2
-
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2
-
10
-
4
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6
-
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1
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1
1
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3
-
-
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1
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2
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-
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-
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286721
Harary
Biosynthesis of dicarboxylic a ...
Triticum aestivum
J. Biol. Chem.
203
595-604
1953
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1
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1
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1
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2
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2
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1
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1
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1
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1
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2
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2
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