Cloned (Comment) | Organism |
---|---|
functional expression of ME1, under the control of cytomegalovirus, which also directs the transcription of GFP from an internal ribosome entry site, in Rattus norvegicus INS-1 832/13 cells and in Mus musculus islets | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | ME1 overexpression results in an increased glucose-dependent rise in malate and citrate levels in INS-1 832/13 cells. Introduction of ME1 activity alters glucose-stimulated insulin secretion to a lesser degree in mouse islets than in INS-1 832/13 cells, overview. In contrast to rat, mouse beta-cells lack ME1 activity, which is suggested to explain their lack of methyl succinate-stimulated insulin secretion. Metabolic phenotypes of transfected cells, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
(S)-malate | recombinant ME1, pH 7.4, temperature not specified in the publication | Homo sapiens | |
5.8 | - |
pyruvate | recombinant ME1, pH 7.4, temperature not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Homo sapiens | - |
pyruvate + NADPH + H+ + CO2 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant ME1 2229fold from INS-1 832/13 cell cytosolic fractions by 2',5'-ADP affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreatic beta cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.92 | - |
recombinant enzyme in INS-1 832/13 cells, reverse reaction, pH 7.4, temperature not specified in the publication | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Homo sapiens | pyruvate + NADPH + H+ + CO2 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
cytosolic malic enzyme | - |
Homo sapiens |
malic enzyme | - |
Homo sapiens |
ME1 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | some pyruvate cycling pathways require malate export from mitochondria and NADP+-dependent decarboxylation of malate to pyruvate by cytosolic malic enzyme ME1. Role of ME1 in glucose-stimulated insulin secretion and in methyl succinate-stimulated insulin secretion occuring occur via succinate entry into the mitochondria in exchange for malate and subsequent malate conversion to pyruvate, overview | Homo sapiens |