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Literature summary for 1.1.1.40 extracted from

  • Detarsio, E.; Maurino, V.G.; Alvarez, C.E.; Mueller, G.L.; Andreo, C.S.; Drincovich, M.F.
    Maize cytosolic NADP-malic enzyme (ZmCytNADP-ME): a phylogenetically distant isoform specifically expressed in embryo and emerging roots (2008), Plant Mol. Biol., 68, 355-367.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
fumarate when assayed at malate concentrations of 0.2 mM, fumarate activates the enzyme by 80% over the control activity Zea mays
additional information after PEG 6000 treatment, the levels of cytosolic NADP-ME transcripts are enhanced by approximately 2.5times Zea mays
succinate when assayed at malate concentrations of 0.2 mM, succinate activates the enzyme by 121% over the control activity, at malate concentration of 1.2 mM, succinate activates the enzyme by 62% over the control activity Zea mays

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Zea mays

Inhibitors

Inhibitors Comment Organism Structure
D-fructose-1,6-bisphosphate when assayed at malate concentrations of 0.2 mM, D-fructose-1,6-bisphosphate inhibits the enzyme by a 49% over the control activity Zea mays
additional information cytosolic NADP-ME expression in roots decreases with development, decreased levels of expression of cytosolic NADP-ME is observed in roots after incubating in solutions of Na2CO3 at pH 11.0 or NaHCO3 at pH 6.5; cytosolic NADP-ME is not inhibited by high malate concentrations at pH 7.0; NADP-ME is not affected by acetyl-CoA, CoA, pyruvate, L-alanine, alpha-ketoglutarate, glycerol-3-phosphate, 3-phospho-glycerate, and citrate Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Zea mays 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
mature monomer of recombinant enzyme after enterokinase digestion, SDS-PAGE Zea mays
71600
-
calculated from amino acid sequence Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays Q5G1U0 inbred line AX882 (Nidera)
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Zea mays
-
root emerging root Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Zea mays pyruvate + CO2 + NADPH
-
?

Subunits

Subunits Comment Organism
hexamer or octamer
-
Zea mays

Synonyms

Synonyms Comment Organism
NADP-malic enzyme
-
Zea mays
NADP-ME
-
Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Zea mays

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Zea mays