Activating Compound | Comment | Organism | Structure |
---|---|---|---|
aspartate | 2fold activation of isozyme NADP-ME1 | Nicotiana tabacum | |
CoA | slight activation of isozyme NADP-ME2 | Nicotiana tabacum | |
additional information | no activation of isozyme NADP-ME2 by aspartate | Nicotiana tabacum |
Cloned (Comment) | Organism |
---|---|
isozyme NADP-ME2, expression in Escherichia coli | Nicotiana tabacum |
nadp-me1, expression in Escherichia coli | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | 40% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 | Nicotiana tabacum | |
ATP | 20% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 | Nicotiana tabacum | |
CoA | 30% inhibition of isozyme NADP-ME1 at 2 mM | Nicotiana tabacum | |
D-glucose 6-phosphate | 40% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 | Nicotiana tabacum | |
fumarate | 20% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 | Nicotiana tabacum | |
malate | inhibition of isozyme NADP-ME1 at pH 7.0 | Nicotiana tabacum | |
additional information | no inhibition of isozyme NADP-ME2 by aspartate and malate | Nicotiana tabacum | |
oxaloacetic acid | 60% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 | Nicotiana tabacum | |
pyruvate | 20% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 | Nicotiana tabacum | |
succinate | inhibition of isozyme NADP-ME2 | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00171 | - |
NADP+ | pH 7.3, isozyme NADP-ME1 | Nicotiana tabacum | |
0.00429 | - |
NADP+ | pH 7.0, isozyme NADP-ME2 | Nicotiana tabacum | |
0.13 | - |
(S)-malate | pH 7.3, isozyme NADP-ME1 | Nicotiana tabacum | |
0.69 | - |
pyruvate | pH 7.0, isozyme NADP-ME1 | Nicotiana tabacum | |
1.46 | - |
(S)-malate | pH 7.0, isozyme NADP-ME2 | Nicotiana tabacum | |
2.6 | - |
pyruvate | pH 7.0, isozyme NADP-ME2 | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Nicotiana tabacum | 9507 | - |
cytosol | - |
Nicotiana tabacum | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
4 * 63000, plastidic isozyme, SDS-PAGE | Nicotiana tabacum |
65000 | - |
4 * 65000, cytosolic isozyme, SDS-PAGE | Nicotiana tabacum |
260000 | - |
native PAGE | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Nicotiana tabacum | - |
pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q006P9 | gene nadp-me2; gene nadp-me2 | - |
Nicotiana tabacum | Q006Q0 | gene nadp-me1; gene nadp-me1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Nicotiana tabacum | - |
leaf | - |
Nicotiana tabacum | - |
root | - |
Nicotiana tabacum | - |
stem | - |
Nicotiana tabacum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.048 | - |
enzyme extracts from flowers | Nicotiana tabacum |
0.069 | - |
enzyme extracts from stems | Nicotiana tabacum |
0.072 | - |
enzyme extracts from leaves | Nicotiana tabacum |
0.571 | - |
enzyme extract from roots | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Nicotiana tabacum | pyruvate + CO2 + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 63000, plastidic isozyme, SDS-PAGE | Nicotiana tabacum |
tetramer | 4 * 65000, cytosolic isozyme, SDS-PAGE | Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
L-malate:NADP oxidoreductase (oxaloacetate decarboxylating) | - |
Nicotiana tabacum |
NADP-malic enzyme | - |
Nicotiana tabacum |
NADP-ME | - |
Nicotiana tabacum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.6 | - |
pyruvate | pH 7.0, isozyme NADP-ME2 | Nicotiana tabacum | |
154.3 | - |
(S)-malate | pH 7.3, isozyme NADP-ME1 | Nicotiana tabacum | |
158.4 | - |
pyruvate | pH 7.0, isozyme NADP-ME1 | Nicotiana tabacum | |
177 | - |
(S)-malate | pH 7.0, isozyme NADP-ME2 | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
isozyme NADP-ME1, reverse reaction | Nicotiana tabacum |
7 | - |
isozyme NADP-ME2, forward and reverse reaction | Nicotiana tabacum |
7.3 | - |
isozyme NADP-ME1, forward reaction | Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Nicotiana tabacum | |
NADPH | - |
Nicotiana tabacum |