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Literature summary for 1.1.1.40 extracted from
- Malic enzymes of Trichomonas vaginalis: two enzyme families, two distinct origins (2004), Gene, 329, 81-92.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme activity is not affected by oxaloacetate, fumarate, succinate, AMP, and NH4+ | Trichomonas vaginalis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| preparation of a genomic library, DNA and amino acid sequence determination and analysis, detailed phylogenetic reconstruction of malic enzymes, overview | Trichomonas vaginalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | 27% inhibition at 0.2 mM | Trichomonas vaginalis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
NADP+ | pH 7.6, 25°C | Trichomonas vaginalis | |
| 0.67 | - |
(S)-malate | pH 7.6, 25°C | Trichomonas vaginalis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | exclusively in | Trichomonas vaginalis | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | strictly dependent on presence of Mg2+ or Mn2+ | Trichomonas vaginalis | |
| Mn2+ | strictly dependent on presence of Mg2+ or Mn2+ | Trichomonas vaginalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42500 | - |
2 * 42500, SDS-PAGE | Trichomonas vaginalis |
| 80000 | - |
gel filtration | Trichomonas vaginalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | Trichomonas vaginalis | responsible for pyruvate and NADPH production | pyruvate + CO2 + NADPH | - |
? | |
| (S)-malate + NADP+ | Trichomonas vaginalis TV 7-37 | responsible for pyruvate and NADPH production | pyruvate + CO2 + NADPH | - |
? | |
| additional information | Trichomonas vaginalis | enzyme belongs to the prokaryotic small subunit-type family of malic enzymes being achieved by a horizontal gene transfer from an eubacterium to the ancestor of Trichomonas vaginalis, enzyme occurs besides the eukaryotic large subunit-type enzyme in the organism | ? | - |
? | |
| additional information | Trichomonas vaginalis TV 7-37 | enzyme belongs to the prokaryotic small subunit-type family of malic enzymes being achieved by a horizontal gene transfer from an eubacterium to the ancestor of Trichomonas vaginalis, enzyme occurs besides the eukaryotic large subunit-type enzyme in the organism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichomonas vaginalis | Q717I9 | - |
- |
| Trichomonas vaginalis TV 7-37 | Q717I9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 331fold to homogeneity by a 4-step chromatographical procedure | Trichomonas vaginalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 199 | - |
purified enzyme | Trichomonas vaginalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Trichomonas vaginalis | pyruvate + CO2 + NADPH | - |
? | |
| (S)-malate + NADP+ | responsible for pyruvate and NADPH production | Trichomonas vaginalis | pyruvate + CO2 + NADPH | - |
? | |
| (S)-malate + NADP+ | - |
Trichomonas vaginalis TV 7-37 | pyruvate + CO2 + NADPH | - |
? | |
| (S)-malate + NADP+ | responsible for pyruvate and NADPH production | Trichomonas vaginalis TV 7-37 | pyruvate + CO2 + NADPH | - |
? | |
| additional information | enzyme belongs to the prokaryotic small subunit-type family of malic enzymes being achieved by a horizontal gene transfer from an eubacterium to the ancestor of Trichomonas vaginalis, enzyme occurs besides the eukaryotic large subunit-type enzyme in the organism | Trichomonas vaginalis | ? | - |
? | |
| additional information | enzyme does not decarboxylate oxaloacetate | Trichomonas vaginalis | ? | - |
? | |
| additional information | enzyme belongs to the prokaryotic small subunit-type family of malic enzymes being achieved by a horizontal gene transfer from an eubacterium to the ancestor of Trichomonas vaginalis, enzyme occurs besides the eukaryotic large subunit-type enzyme in the organism | Trichomonas vaginalis TV 7-37 | ? | - |
? | |
| additional information | enzyme does not decarboxylate oxaloacetate | Trichomonas vaginalis TV 7-37 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42500, SDS-PAGE | Trichomonas vaginalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| malic enzyme | - |
Trichomonas vaginalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Trichomonas vaginalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Trichomonas vaginalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NAD+ | Trichomonas vaginalis | |
| NADP+ | absolute specific for | Trichomonas vaginalis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Trichomonas vaginalis | calculated | - |
6 |
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