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Literature summary for 1.1.1.40 extracted from

  • Shearer, H.L.; Turpin, D.H.; Dennis, D.T.
    Characterization of NADP-dependent malic enzyme from developing castor oil seed endosperm (2004), Arch. Biochem. Biophys., 429, 134-144.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acetyl carnitine 19% activation at 5 mM Ricinus communis
acetyl-CoA 24% activation at 0.005 mM Ricinus communis
ATP 18% activation at 5 mM Ricinus communis
citrate 22% activation at 5 mM Ricinus communis
CoA 29% activation at 0.005 mM Ricinus communis
L-glutamate 15% activation at 5 mM Ricinus communis
malonyl-CoA 15% activation at 0.005 mM Ricinus communis
palmitoyl-CoA 69% activation at 0.005 mM Ricinus communis
succinate 65% activation at 5 mM Ricinus communis
UDP 10% activation at 5 mM Ricinus communis

Inhibitors

Inhibitors Comment Organism Structure
AMP 41% inhibition at 5 mM Ricinus communis
cAMP 10% inhibition at 5 mM Ricinus communis
CO2 product inhibition, uncompetitive with respect to L-malate and NADP+, 39% inhibition at 25 mM Ricinus communis
D-fructose-1,6-bisphosphate 13% inhibition at 5 mM Ricinus communis
GDP 13% inhibition at 5 mM Ricinus communis
NADPH product inhibition, competitive with respect to L-malate and NADP+ Ricinus communis
oxalate 51% inhibition at 1 mM Ricinus communis
phosphoenolpyruvate 39% inhibition at 5 mM Ricinus communis
pyruvate product inhibition, competitive with respect to L-malate, 16% inhibition at 5 mM Ricinus communis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0103
-
NADP+ pH 7.6-7.7, 25°C Ricinus communis
0.22
-
L-malate pH 7.6-7.7, 25°C Ricinus communis

Localization

Localization Comment Organism GeneOntology No. Textmining
leucoplast
-
Ricinus communis 9516
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enzyme requires either Mg2+ or Mn2+ for activity, no activity without divalent cation Ricinus communis
Mn2+ enzyme requires either Mg2+ or Mn2+ for activity, no activity without divalent cation Ricinus communis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
4 * 63000, approximately, SDS-PAGE Ricinus communis
254000
-
gel filtration Ricinus communis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Ricinus communis enzyme is involved in fatty acid biosynthesis in oil seed leucoplasts, overview pyruvate + CO2 + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
cv. Baker 296
-

Purification (Commentary)

Purification (Comment) Organism
5160fold from oil seed Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining
endosperm developing, maximal at stage 7 of endosperm development, overview Ricinus communis
-
seed
-
Ricinus communis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.2
-
purified enzyme Ricinus communis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+ enzyme is involved in fatty acid biosynthesis in oil seed leucoplasts, overview Ricinus communis pyruvate + CO2 + NADPH
-
r
(S)-malate + NADP+ decarboxylation is favoured Ricinus communis pyruvate + CO2 + NADPH
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 63000, approximately, SDS-PAGE Ricinus communis

Synonyms

Synonyms Comment Organism
NADP-dependent malic enzyme
-
Ricinus communis
NADP-ME
-
Ricinus communis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at, both reaction directions Ricinus communis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44
-
stable for at least 10 min Ricinus communis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 7.7 both reaction directions Ricinus communis

pH Range

pH Minimum pH Maximum Comment Organism
7 8.2 broad range, 80% of maximal activity at pH 7.0 and pH 8.2 Ricinus communis

Cofactor

Cofactor Comment Organism Structure
NADP+ absolutely specific for, no activity with NAD+ Ricinus communis
NADPH
-
Ricinus communis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Ricinus communis
0.016
-
pyruvate pH 7.6-7.7, 25°C Ricinus communis
0.046
-
NADPH versus NADP+, pH 7.6-7.7, 25°C Ricinus communis
0.372
-
CO2 versus NADP+, pH 7.6-7.7, 25°C Ricinus communis
0.492
-
NADPH versus L-malate, pH 7.6-7.7, 25°C Ricinus communis
0.83
-
CO2 versus L-malate, pH 7.6-7.7, 25°C Ricinus communis