Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl carnitine | 19% activation at 5 mM | Ricinus communis | |
acetyl-CoA | 24% activation at 0.005 mM | Ricinus communis | |
ATP | 18% activation at 5 mM | Ricinus communis | |
citrate | 22% activation at 5 mM | Ricinus communis | |
CoA | 29% activation at 0.005 mM | Ricinus communis | |
L-glutamate | 15% activation at 5 mM | Ricinus communis | |
malonyl-CoA | 15% activation at 0.005 mM | Ricinus communis | |
palmitoyl-CoA | 69% activation at 0.005 mM | Ricinus communis | |
succinate | 65% activation at 5 mM | Ricinus communis | |
UDP | 10% activation at 5 mM | Ricinus communis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 41% inhibition at 5 mM | Ricinus communis | |
cAMP | 10% inhibition at 5 mM | Ricinus communis | |
CO2 | product inhibition, uncompetitive with respect to L-malate and NADP+, 39% inhibition at 25 mM | Ricinus communis | |
D-fructose-1,6-bisphosphate | 13% inhibition at 5 mM | Ricinus communis | |
GDP | 13% inhibition at 5 mM | Ricinus communis | |
NADPH | product inhibition, competitive with respect to L-malate and NADP+ | Ricinus communis | |
oxalate | 51% inhibition at 1 mM | Ricinus communis | |
phosphoenolpyruvate | 39% inhibition at 5 mM | Ricinus communis | |
pyruvate | product inhibition, competitive with respect to L-malate, 16% inhibition at 5 mM | Ricinus communis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0103 | - |
NADP+ | pH 7.6-7.7, 25°C | Ricinus communis | |
0.22 | - |
L-malate | pH 7.6-7.7, 25°C | Ricinus communis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
leucoplast | - |
Ricinus communis | 9516 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | enzyme requires either Mg2+ or Mn2+ for activity, no activity without divalent cation | Ricinus communis | |
Mn2+ | enzyme requires either Mg2+ or Mn2+ for activity, no activity without divalent cation | Ricinus communis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
4 * 63000, approximately, SDS-PAGE | Ricinus communis |
254000 | - |
gel filtration | Ricinus communis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Ricinus communis | enzyme is involved in fatty acid biosynthesis in oil seed leucoplasts, overview | pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ricinus communis | - |
cv. Baker 296 | - |
Purification (Comment) | Organism |
---|---|
5160fold from oil seed | Ricinus communis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | developing, maximal at stage 7 of endosperm development, overview | Ricinus communis | - |
seed | - |
Ricinus communis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.2 | - |
purified enzyme | Ricinus communis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | enzyme is involved in fatty acid biosynthesis in oil seed leucoplasts, overview | Ricinus communis | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | decarboxylation is favoured | Ricinus communis | pyruvate + CO2 + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 63000, approximately, SDS-PAGE | Ricinus communis |
Synonyms | Comment | Organism |
---|---|---|
NADP-dependent malic enzyme | - |
Ricinus communis |
NADP-ME | - |
Ricinus communis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, both reaction directions | Ricinus communis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
44 | - |
stable for at least 10 min | Ricinus communis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 7.7 | both reaction directions | Ricinus communis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.2 | broad range, 80% of maximal activity at pH 7.0 and pH 8.2 | Ricinus communis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | absolutely specific for, no activity with NAD+ | Ricinus communis | |
NADPH | - |
Ricinus communis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Ricinus communis | |
0.016 | - |
pyruvate | pH 7.6-7.7, 25°C | Ricinus communis | |
0.046 | - |
NADPH | versus NADP+, pH 7.6-7.7, 25°C | Ricinus communis | |
0.372 | - |
CO2 | versus NADP+, pH 7.6-7.7, 25°C | Ricinus communis | |
0.492 | - |
NADPH | versus L-malate, pH 7.6-7.7, 25°C | Ricinus communis | |
0.83 | - |
CO2 | versus L-malate, pH 7.6-7.7, 25°C | Ricinus communis |