Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.40 extracted from

  • Goodenough, P.W.; Prosser, I.M.; Young, K.
    NADP-linked malic enzyme and malate metabolism in ageing tomato fruit (1985), Phytochemistry, 24, 1157-1162.
No PubMed abstract available
Search for more literature for 1.1.1.40

Activating Compound

Activating Compound Comment Organism Structure
fumarate slight activation at low concentration Solanum lycopersicum
succinate slight activation up to 10 mM Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate strong Solanum lycopersicum
fumarate at high concentration Solanum lycopersicum
oxaloacetate strong Solanum lycopersicum
pyruvate non-competitive inhibition Solanum lycopersicum
succinate slight inhibition at high concentrations Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 NADP+
-
 Solanum lycopersicum
0.53
-
 (S)-malate
-
 Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Solanum lycopersicum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement for a divalent cation, Mn2+ or Mg2+ Solanum lycopersicum
Mg2+ Km: 0.315 mM Solanum lycopersicum
Mn2+ Km: 0.210 mM Solanum lycopersicum
Mn2+ absolute requirement for a divalent cation, Mn2+ or Mg2+ Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
260000 265000 gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum lycopersicum the specific activity falls rapidly as the fruit ripens ?
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
 Solanum lycopersicum pyruvate + CO2 + NADPH
-
 r
additional information the specific activity falls rapidly as the fruit ripens Solanum lycopersicum ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 64000-65000, SDS-PAGE Solanum lycopersicum