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Literature summary for 1.1.1.40 extracted from
- Characterization of two members of a novel malic enzyme class (1999), Biochim. Biophys. Acta, 1432, 275-285.
General Stability
| General Stability | Organism |
|---|---|
| loss of activity upon repeated freezing and thawing | Sinorhizobium meliloti |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-malate | - |
Sinorhizobium meliloti |  |
| D-Tartrate | - |
Sinorhizobium meliloti | |
| glyoxylate | - |
Sinorhizobium meliloti | |
| L-Tartrate | - |
Sinorhizobium meliloti | |
| malonate | - |
Sinorhizobium meliloti | |
| Tartronate | - |
Sinorhizobium meliloti | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.6 | - |
L-malate | - |
Sinorhizobium meliloti | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 82000 | - |
x * 82000, SDS-PAGE | Sinorhizobium meliloti |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinorhizobium meliloti | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sinorhizobium meliloti |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 49 | - |
- |
Sinorhizobium meliloti |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several weeks | Sinorhizobium meliloti |
| 4°C, loss of activity upon prolonged storage | Sinorhizobium meliloti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Sinorhizobium meliloti | pyruvate + CO2 + NADPH | - |
ir | |
| Oxaloacetate | no activity | Sinorhizobium meliloti | Pyruvate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 82000, SDS-PAGE | Sinorhizobium meliloti |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 582 | - |
L-malate | assuming an octameric oligomerization state | Sinorhizobium meliloti | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Sinorhizobium meliloti | |
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Release 2023.1 (January 2023)
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