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Literature summary for 1.1.1.4 extracted from

  • Zhang, L.; Xu, Q.; Peng, X.; Xu, B.; Wu, Y.; Yang, Y.; Sun, S.; Hu, K.; Shen, Y.
    Cloning, expression and characterization of glycerol dehydrogenase involved in 2,3-butanediol formation in Serratia marcescens H30 (2014), J. Ind. Microbiol. Biotechnol., 41, 1319-1327.
    View publication on PubMed
Search for more literature for 1.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
gene gldA, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3) Serratia marcescens

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibits (3S/3R)-acetoin reduction Serratia marcescens
Fe2+ strongly inhibits (3S/3R)-acetoin reduction by over 85% Serratia marcescens
Fe3+ strongly inhibits (3S/3R)-acetoin reduction by over 85% Serratia marcescens
Zn2+ inhibits (3S/3R)-acetoin reduction Serratia marcescens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ GDH activity is significantly enhanced to 784% for meso-2,3-butanediol oxidation in the presence of Fe2+ Serratia marcescens
Mg2+ enhances (3S/3R)-acetoin reduction Serratia marcescens
Mn2+ GDH activity is significantly enhanced for (3S/3R)-acetoin reduction in the presence of Mn2+ Serratia marcescens
Na+ enhances (3S/3R)-acetoin reduction Serratia marcescens
NH4+ enhances (3S/3R)-acetoin reduction Serratia marcescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 x * 39000, recombinant enzyme, SDS-PAGE Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
-
-
Serratia marcescens H30
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.48
-
 crude enzyme, with substrate (3S/3R)-acetoin and NADH at room temperature, pH 8.0, 22°C Serratia marcescens
20.75
-
 purified enzyme, with substrate (3S/3R)-acetoin and NADH at room temperature, pH 8.0, 22°C Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R,3S)-acetoin + NADH the enzyme shows lower Km value and higher catalytic efficiency for (3S/3R)-acetoin in comparison to those for (2R,3R)-2,3-butanediol and meso-2,3-butanediol, the reduction reaction is preferred, low activity with (2R,3R)-2,3-butanediol Serratia marcescens (2R,3R)-2,3-butanediol + meso-2,3-butanediol + NAD+
-
 r
(3R,3S)-acetoin + NADH the enzyme shows lower Km value and higher catalytic efficiency for (3S/3R)-acetoin in comparison to those for (2R,3R)-2,3-butanediol and meso-2,3-butanediol, the reduction reaction is preferred, low activity with (2R,3R)-2,3-butanediol Serratia marcescens H30 (2R,3R)-2,3-butanediol + meso-2,3-butanediol + NAD+
-
 r
1,4-butanediol + NAD+ low activity Serratia marcescens ?
-
 r
1,4-butanediol + NAD+ low activity Serratia marcescens H30 ?
-
 r
diacetyl + NADH + H+ diacetyl can be converted into 2,3-butanediol via acetoin by the enzyme Serratia marcescens acetoin + NAD+
-
 ?
diacetyl + NADH + H+ diacetyl can be converted into 2,3-butanediol via acetoin by the enzyme Serratia marcescens H30 acetoin + NAD+
-
 ?
isopropanol + NAD+ low activity Serratia marcescens ?
-
 r
isopropanol + NAD+ low activity Serratia marcescens H30 ?
-
 r
additional information the purified enzyme glycerol dehydrogenase, GDH EC 1.1.1.6, also catalyzes the interconversion of (3S)-acetoin/meso-2,3-butanediol and (3R)-acetoin/(2R,3R)-2,3-butanediol. (2S,3S)-2,3-Butanediol is not a substrate for the GDH at all. Also no activity with 1-propanol, 1-butanol, isopentanol, ethylene glycol, ethanol, and (3S/3R)-acetoin in the oxidation reaction, and poor activity with formaldehyde in the reduction reaction Serratia marcescens ?
-
 ?
additional information the purified enzyme glycerol dehydrogenase, GDH EC 1.1.1.6, also catalyzes the interconversion of (3S)-acetoin/meso-2,3-butanediol and (3R)-acetoin/(2R,3R)-2,3-butanediol. (2S,3S)-2,3-Butanediol is not a substrate for the GDH at all. Also no activity with 1-propanol, 1-butanol, isopentanol, ethylene glycol, ethanol, and (3S/3R)-acetoin in the oxidation reaction, and poor activity with formaldehyde in the reduction reaction Serratia marcescens H30 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, recombinant enzyme, SDS-PAGE Serratia marcescens

Synonyms

Synonyms Comment Organism
2,3-butanediol dehydrogenase
-
 Serratia marcescens
acetoin/diacetyl reductase
-
 Serratia marcescens
BDH
-
 Serratia marcescens
More cf. EC 1.1.1.6, glycerol dehydrogenase Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Serratia marcescens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 temperature profile, overview Serratia marcescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 over 60% of maximal activity is retained Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 diacetyl reduction Serratia marcescens
8
-
 reduction of (3S/3R)-acetoin and oxidations of meso-2,3-butanediol and glycerol Serratia marcescens

pH Range

pH Minimum pH Maximum Comment Organism
5 9 temperature profile, overview Serratia marcescens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Serratia marcescens
NADH
-
 Serratia marcescens

General Information

General Information Comment Organism
evolution the Serratia marcescens enzyme belongs to the type III Fe-ADH superfamily, three consecutive glycine residues belong to a 14-amino acid residue motif (GDK motif) as the coenzyme NAD(H) binding site, and three conserved histidine residues belong to a 16-residue segment that is homologous to the 15-residue stretch as the binding site of metal Serratia marcescens