Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Serratia marcescens |
expressed in Serratia marcescens MG1 slaC mutant strain | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE | Bacillus subtilis |
35000 | - |
x * 35000, SDS-PAGE | Serratia marcescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-acetoin + NADH + H+ | Bacillus subtilis | - |
(R,R)-butane-2,3-diol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | Serratia marcescens | - |
(R,R)-butane-2,3-diol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | Bacillus subtilis 168 | - |
(R,R)-butane-2,3-diol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | Serratia marcescens MG1 | - |
(R,R)-butane-2,3-diol + NAD+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | Bacillus subtilis | - |
(R)-acetoin + NADH + H+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | Serratia marcescens | - |
(R)-acetoin + NADH + H+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | Bacillus subtilis 168 | - |
(R)-acetoin + NADH + H+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | Serratia marcescens MG1 | - |
(R)-acetoin + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis 168 | - |
- |
- |
Serratia marcescens | - |
- |
- |
Serratia marcescens MG1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography | Bacillus subtilis |
His-Trap column chromatography | Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-acetoin + NADH + H+ | - |
Bacillus subtilis | (R,R)-butane-2,3-diol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | - |
Serratia marcescens | (R,R)-butane-2,3-diol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | - |
Bacillus subtilis 168 | (R,R)-butane-2,3-diol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | - |
Serratia marcescens MG1 | (R,R)-butane-2,3-diol + NAD+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | - |
Bacillus subtilis | (R)-acetoin + NADH + H+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | - |
Serratia marcescens | (R)-acetoin + NADH + H+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | - |
Bacillus subtilis 168 | (R)-acetoin + NADH + H+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | - |
Serratia marcescens MG1 | (R)-acetoin + NADH + H+ | - |
r | |
diacetyl + NADH + H+ | - |
Bacillus subtilis | (3S)-acetoin + NAD+ | - |
r | |
diacetyl + NADH + H+ | - |
Serratia marcescens | (3S)-acetoin + NAD+ | - |
r | |
diacetyl + NADH + H+ | - |
Bacillus subtilis 168 | (3S)-acetoin + NAD+ | - |
r | |
diacetyl + NADH + H+ | - |
Serratia marcescens MG1 | (3S)-acetoin + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Bacillus subtilis |
? | x * 35000, SDS-PAGE | Serratia marcescens |
Synonyms | Comment | Organism |
---|---|---|
(2R,3R)-2,3-butanediol dehydrogenase | - |
Bacillus subtilis |
BdhA | - |
Bacillus subtilis |
meso-2,3-butanediol dehydrogenase | - |
Serratia marcescens |
SLAC | - |
Serratia marcescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis | |
NAD+ | - |
Serratia marcescens | |
NADH | - |
Bacillus subtilis | |
NADH | - |
Serratia marcescens |