BRENDA - Enzyme Database show
show all sequences of 1.1.1.4

Properties of 2,3-butanediol dehydrogenase from Lactococcus lactis subsp. lactis in relation to citrate fermentation

Crow, V.L.; Appl. Environ. Microbiol. 56, 1656-1665 (1990)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
170000
-
enzyme 1 and 2, gel filtration
Lactococcus lactis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactococcus lactis
-
2,3-butanediol dehydrogenase 1 and 2; subsp. lactis D10
-
Purification (Commentary)
Commentary
Organism
2,3-butanediol dehydrogenase 1 and 2; partially
Lactococcus lactis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.1
-
acetoin, enzyme 1
Lactococcus lactis
7.55
-
acetoin, enzyme 2
Lactococcus lactis
35.55
-
diacetyl, enzyme 2
Lactococcus lactis
35.6
-
diacetyl, enzyme 1
Lactococcus lactis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-butanediol + NAD+
-
246411
Lactococcus lactis
acetoin + NADH
-
246411
Lactococcus lactis
r
acetoin + NADH + H+
-
246411
Lactococcus lactis
2,3-butanediol + NAD+
enzyme 1, only meso-2,3-butanediol is formed, enzyme 2 gives mixture of meso- and optical isomers
246411
Lactococcus lactis
r
diacetyl + NADH
-
246411
Lactococcus lactis
2,3-butanediol + NAD+
enzyme 1, only meso-2,3-butanediol is formed, enzyme 2 gives mixture of meso- and optical isomers
246411
Lactococcus lactis
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
-
substrate reduction, enzyme 1
Lactococcus lactis
6.1
-
substrate reduction, enzyme 2
Lactococcus lactis
8.5
-
substrate oxidation, enzyme 2
Lactococcus lactis
10
-
substrate oxidation, enzyme 1
Lactococcus lactis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
170000
-
enzyme 1 and 2, gel filtration
Lactococcus lactis
Purification (Commentary) (protein specific)
Commentary
Organism
2,3-butanediol dehydrogenase 1 and 2; partially
Lactococcus lactis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.1
-
acetoin, enzyme 1
Lactococcus lactis
7.55
-
acetoin, enzyme 2
Lactococcus lactis
35.55
-
diacetyl, enzyme 2
Lactococcus lactis
35.6
-
diacetyl, enzyme 1
Lactococcus lactis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-butanediol + NAD+
-
246411
Lactococcus lactis
acetoin + NADH
-
246411
Lactococcus lactis
r
acetoin + NADH + H+
-
246411
Lactococcus lactis
2,3-butanediol + NAD+
enzyme 1, only meso-2,3-butanediol is formed, enzyme 2 gives mixture of meso- and optical isomers
246411
Lactococcus lactis
r
diacetyl + NADH
-
246411
Lactococcus lactis
2,3-butanediol + NAD+
enzyme 1, only meso-2,3-butanediol is formed, enzyme 2 gives mixture of meso- and optical isomers
246411
Lactococcus lactis
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
-
substrate reduction, enzyme 1
Lactococcus lactis
6.1
-
substrate reduction, enzyme 2
Lactococcus lactis
8.5
-
substrate oxidation, enzyme 2
Lactococcus lactis
10
-
substrate oxidation, enzyme 1
Lactococcus lactis
Other publictions for EC 1.1.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737512
Rados
Stereospecificity of Corynebac ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC13032
Appl. Microbiol. Biotechnol.
100
10573-10583
2016
-
-
1
-
-
-
-
4
-
-
-
4
-
9
-
-
1
-
-
-
-
-
10
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
-
4
-
-
-
1
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
4
4
737519
de Oliveira
Synthetic operon for (R,R)-2,3 ...
Bacillus subtilis, Bacillus subtilis WN1038
Appl. Microbiol. Biotechnol.
100
719-728
2016
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738345
Ge
-
Contracted but effective: Prod ...
Bacillus licheniformis, Bacillus licheniformis MW3
Green Chem.
18
4693-4703
2016
-
-
1
-
-
-
-
-
-
-
-
4
-
2
-
-
1
-
-
-
14
-
20
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
14
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739694
Bae
Efficient production of acetoi ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
Sci. Rep.
6
27667
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
46
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737847
Kang
Enhanced production of 2,3-but ...
Bacillus sp., Bacillus sp. BRC1
Bioprocess Biosyst. Eng.
38
299-305
2015
-
-
1
-
-
-
-
4
-
1
-
2
-
2
-
-
1
-
-
-
-
-
14
-
1
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
1
-
2
-
-
-
1
-
-
-
-
14
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
738800
Bai
Engineered Serratia marcescens ...
Bacillus subtilis 168, Bacillus subtilis, Serratia marcescens, Serratia marcescens MG1
J. Ind. Microbiol. Biotechnol.
42
779-786
2015
-
-
2
-
-
-
-
-
-
-
-
8
-
77
-
-
2
-
-
-
-
-
12
2
-
-
-
-
-
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
-
-
8
-
-
-
2
-
-
-
-
12
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738953
Zhao
Identification and characteriz ...
Corynebacterium crenatum, Corynebacterium crenatum SYPA5-5
Lett. Appl. Microbiol.
61
573-579
2015
-
-
1
-
-
-
4
-
-
5
-
-
-
5
-
-
1
-
-
-
-
-
-
1
1
-
1
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
4
-
-
-
5
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
2
-
-
-
-
-
-
-
-
-
739127
Yu
Characterization of a (2R,3R)- ...
Rhodococcus erythropolis, Rhodococcus erythropolis WZ010
Molecules
20
7156-7173
2015
1
-
1
-
-
-
15
4
-
3
1
3
-
4
-
-
1
-
-
-
-
-
19
1
2
-
1
4
2
1
-
2
-
-
-
1
-
1
2
-
-
-
-
15
-
4
-
3
1
3
-
-
-
1
-
-
-
-
19
1
2
-
1
4
2
1
-
-
-
-
-
-
4
4
739571
Maddock
Substitutions at the cofactor ...
Clostridium autoethanogenum
Protein Eng. Des. Sel.
28
251-258
2015
-
-
1
-
7
-
-
21
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
21
-
-
-
2
-
-
-
-
-
1
2
-
7
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
21
-
-
-
-
-
-
-
-
21
21
737501
Koepke
Reconstruction of an acetogeni ...
Clostridium autoethanogenum
Appl. Environ. Microbiol.
80
3394-3403
2014
-
-
-
-
-
-
-
5
-
-
-
-
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
5
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
5
-
-
-
-
-
-
-
-
5
5
738472
Cui
Utilization of excess NADH in ...
Bacillus licheniformis
J. Appl. Microbiol.
117
690-698
2014
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738637
Wang
Glycerol dehydrogenase plays a ...
Klebsiella pneumoniae, Klebsiella pneumoniae ATCC 25955
J. Biol. Chem.
289
6080-6090
2014
-
-
1
-
-
-
-
8
-
-
-
2
-
2
-
-
1
-
-
-
-
-
10
1
-
-
-
8
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
8
-
-
-
2
-
-
-
1
-
-
-
-
10
1
-
-
-
8
-
-
-
-
1
-
-
1
7
7
738878
Kochius
-
Enantioselective enzymatic syn ...
Haloarcula marismortui
J. Mol. Catal. B
103
61-66
2014
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738880
Hao
-
Biochemical characterization o ...
Bacillus subtilis, Bacillus subtilis 168
J. Mol. Catal. B
109
184-190
2014
-
-
-
-
1
-
-
-
-
-
-
4
-
72
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722559
Gao
A 2,3-butanediol dehydrogenase ...
Paenibacillus polymyxa, Paenibacillus polymyxa ZJ-9
J. Basic Microbiol.
53
733-741
2013
-
-
1
-
-
-
-
6
-
-
2
-
-
3
-
-
1
-
-
1
1
-
4
1
1
-
-
-
2
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
6
-
-
2
-
-
-
-
1
-
1
1
-
4
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
737366
Miao
Crystallization and preliminar ...
Bacillus coagulans, Bacillus coagulans 2-6
Acta Crystallogr. Sect. F
69
1140-1142
2013
-
-
1
1
-
-
-
-
-
1
-
2
-
4
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721345
Yu
Novel (2R,3R)-2,3-butanediol d ...
Paenibacillus polymyxa, Paenibacillus polymyxa ATCC 12321
Appl. Environ. Microbiol.
77
4230-4233
2011
-
-
1
-
-
-
-
5
-
-
-
2
-
3
-
-
-
-
-
-
-
-
14
-
1
-
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
5
-
-
-
2
-
-
-
-
-
-
-
-
14
-
1
-
-
-
2
-
-
-
-
1
1
-
-
-
721872
Takeda
Identification and characteriz ...
Mycobacterium gilvum, Mycobacterium smegmatis, Mycobacterium sp., Mycobacterium sp. B-009, Mycobacterium vanbaalenii
Biosci. Biotechnol. Biochem.
75
2384-2389
2011
-
-
4
-
-
-
5
1
-
-
-
4
-
8
-
-
1
-
-
-
-
-
14
1
1
-
1
-
2
-
1
2
-
1
-
-
-
4
2
-
-
-
-
5
-
1
-
-
-
4
-
-
-
1
-
-
-
-
14
1
1
-
1
-
2
-
1
1
-
4
4
-
-
-
710944
Gonzalez
Role of Saccharomyces cerevisi ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
76
670-679
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
695776
Ehsani
Engineering of 2,3-butanediol ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
75
3196-3205
2009
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696883
Ehsani
Reversal of coenzyme specifici ...
Saccharomyces cerevisiae
Biotechnol. Bioeng.
104
381-389
2009
-
-
-
-
3
-
-
8
-
-
-
-
-
4
-
-
-
-
-
-
8
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
8
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685764
Ji
Enhanced 2,3-butanediol produc ...
Klebsiella oxytoca, Klebsiella oxytoca ME-303
Biotechnol. Lett.
30
731-734
2008
-
1
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695754
Nicholson
The Bacillus subtilis ydjL (bd ...
Bacillus subtilis
Appl. Environ. Microbiol.
74
6832-6838
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685903
Cabral
Citrate metabolism by Enteroco ...
Enterococcus durans, Enterococcus durans Ov 421, Enterococcus faecium, Enterococcus faecium ET C9
Can. J. Microbiol.
53
607-615
2007
-
-
-
-
-
-
-
-
-
-
-
4
-
8
-
-
-
-
-
2
2
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
2
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667266
Machielsen
Production and characterizatio ...
Pyrococcus furiosus
Appl. Environ. Microbiol.
72
233-238
2006
-
-
1
-
-
-
-
4
-
-
2
-
-
1
-
-
1
-
-
-
-
-
7
1
1
-
1
-
2
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
4
-
-
2
-
-
-
-
1
-
-
-
-
7
1
1
-
1
-
2
-
-
-
-
-
-
-
-
-
655087
Marwoto
-
Enhancement of (R,R)-2,3-butan ...
Paenibacillus polymyxa
Biotechnol. Lett.
24
109-114
2002
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246409
Gonzalez
Characterization and functiona ...
Saccharomyces cerevisiae
Chem. Biol. Interact.
130
425-434
2001
-
-
1
-
-
-
1
5
-
-
-
-
-
2
-
-
1
-
-
-
-
-
3
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
5
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
246413
Gonzalez
Characterization of a (2R,3R)- ...
Saccharomyces cerevisiae
J. Biol. Chem.
275
35876-35885
2000
-
-
1
-
-
-
-
-
-
-
2
-
-
2
-
-
1
-
-
-
1
-
5
1
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
-
5
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
246412
Carballo
Purification, characterization ...
Enterobacter aerogenes
Eur. J. Biochem.
198
327-332
1991
-
-
-
-
-
-
-
8
-
-
2
-
-
1
-
-
1
-
-
-
1
-
6
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
8
-
-
2
-
-
-
-
1
-
-
1
-
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
246410
Heidlas
Purification and characterizat ...
Saccharomyces cerevisiae
Arch. Microbiol.
154
267-273
1990
-
-
-
1
-
-
3
-
-
2
2
-
-
2
-
-
-
-
-
-
-
-
11
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
2
2
-
-
-
-
-
-
-
-
-
11
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
246411
Crow
Properties of 2,3-butanediol d ...
Lactococcus lactis
Appl. Environ. Microbiol.
56
1656-1665
1990
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
4
-
3
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
4
-
3
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
246406
Nokhal
-
Fermentation enzymes in strain ...
Paracoccus denitrificans
Arch. Microbiol.
145
197-201
1986
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246404
Sadaharu
-
Stereospecific and electrophor ...
Bacillus sp., Brevibacillus sp., Enterobacter sp., Micrococcus sp., Pseudomonas sp.
J. Ferment. Technol.
61
467-471
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246407
Höhn-Bentz
Bacterial 2,3-butanediol dehyd ...
Aeromonas hydrophila, Bacillus subtilis, Gluconobacter oxydans, Paenibacillus polymyxa
Arch. Microbiol.
116
197-203
1978
-
-
-
-
-
-
1
7
-
4
1
-
-
4
-
-
1
-
-
-
2
1
8
-
2
-
1
-
2
-
1
8
-
-
-
-
-
-
8
-
-
-
-
1
-
7
-
4
1
-
-
-
-
1
-
-
2
1
8
-
2
-
1
-
2
-
1
-
-
-
-
-
-
-