Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | sodium acetate, acetyl phosphate, acetyl coenzyme A, glutamate, histidine, adenosine mono-, di-, and triphosphates, dithiothreitol, N-ethylmaleimide or iodoacetate at a concentration of 0.01-1 mM have no effect on the rate of the reaction | Micrococcus aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | inhibitor of the reverse reaction | Micrococcus aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
2-oxoglutarate | at pH 6.5 and 25°C | Micrococcus aerogenes | |
0.3 | - |
NADH | at pH 6.5 and 25°C | Micrococcus aerogenes | |
1 | - |
NAD+ | at pH 8.5 and 25°C | Micrococcus aerogenes | |
10 | - |
(R)-2-hydroxyglutarate | at pH 8.5 and 25°C | Micrococcus aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | 78000 | gel filtration | Micrococcus aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-hydroxyglutarate + NAD+ | Micrococcus aerogenes | - |
2-oxoglutarate + NADH + H+ | - |
r | |
(R)-2-hydroxyglutarate + NAD+ | Micrococcus aerogenes 228 | - |
2-oxoglutarate + NADH + H+ | - |
r | |
2-oxoglutarate + NADH + H+ | Micrococcus aerogenes | - |
(R)-2-hydroxyglutarate + NAD+ | - |
r | |
2-oxoglutarate + NADH + H+ | Micrococcus aerogenes 228 | - |
(R)-2-hydroxyglutarate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus aerogenes | - |
- |
- |
Micrococcus aerogenes 228 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and hydroxylapatite column chromatography | Micrococcus aerogenes |
Storage Stability | Organism |
---|---|
-20°C, high protein concentrations (1 mg/ml or more), several months, no loss of activity | Micrococcus aerogenes |
0°C, high protein concentrations (1 mg/ml or more), several weeks, no loss of activity | Micrococcus aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-hydroxyglutarate + NAD+ | - |
Micrococcus aerogenes | 2-oxoglutarate + NADH + H+ | - |
r | |
(R)-2-hydroxyglutarate + NAD+ | - |
Micrococcus aerogenes 228 | 2-oxoglutarate + NADH + H+ | - |
r | |
2-oxoglutarate + NADH + H+ | - |
Micrococcus aerogenes | (R)-2-hydroxyglutarate + NAD+ | - |
r | |
2-oxoglutarate + NADH + H+ | - |
Micrococcus aerogenes 228 | (R)-2-hydroxyglutarate + NAD+ | - |
r | |
additional information | NADPH is oxidized in the presence of 2-oxoglutarate. The rate of this reaction is 3 to 4% of that observed with NADH | Micrococcus aerogenes | ? | - |
? | |
additional information | oxaloacetate, pyruvate, 2-oxobutyrate, 2-oxoisovalerate, and glyoxalate are not reduced by the enzyme | Micrococcus aerogenes | ? | - |
? | |
additional information | NADPH is oxidized in the presence of 2-oxoglutarate. The rate of this reaction is 3 to 4% of that observed with NADH | Micrococcus aerogenes 228 | ? | - |
? | |
additional information | oxaloacetate, pyruvate, 2-oxobutyrate, 2-oxoisovalerate, and glyoxalate are not reduced by the enzyme | Micrococcus aerogenes 228 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 36000-40000, SDS-PAGE | Micrococcus aerogenes |
Synonyms | Comment | Organism |
---|---|---|
alpha-ketoglutarate reductase | - |
Micrococcus aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Micrococcus aerogenes |