Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
sulfoquinovose | with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication | Pseudomonas putida | |
2.4 | - |
sulfoquinovose | with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas putida | the enzyme shows no activity with D-glucose-6-phosphate | ? | - |
? | |
additional information | Pseudomonas putida SQ1 | the enzyme shows no activity with D-glucose-6-phosphate | ? | - |
? | |
sulfoquinovose + NAD+ | Pseudomonas putida | sulfoquinovose is 6-deoxy-6-sulfoglucose | 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
sulfoquinovose + NAD+ | Pseudomonas putida SQ1 | sulfoquinovose is 6-deoxy-6-sulfoglucose | 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida SQ1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows no activity with D-glucose-6-phosphate | Pseudomonas putida | ? | - |
? | |
additional information | the enzyme shows no activity with D-glucose-6-phosphate | Pseudomonas putida SQ1 | ? | - |
? | |
sulfoquinovose + NAD+ | sulfoquinovose is 6-deoxy-6-sulfoglucose | Pseudomonas putida | 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
sulfoquinovose + NAD+ | sulfoquinovose is 6-deoxy-6-sulfoglucose | Pseudomonas putida SQ1 | 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
sulfoquinovose + NADP+ | when NADP+ is used as an electron acceptor, the enzyme activity is 4% of the activity with NAD+ | Pseudomonas putida | 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
sulfoquinovose + NADP+ | when NADP+ is used as an electron acceptor, the enzyme activity is 4% of the activity with NAD+ | Pseudomonas putida SQ1 | 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SQ dehydrogenase | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
sulfoquinovose | with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication | Pseudomonas putida | |
33.8 | - |
sulfoquinovose | with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Pseudomonas putida | |
NADP+ | when NADP+ is used as an electron acceptor, the enzyme activity is 4% of the activity with NAD+ | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
sulfoquinovose | with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication | Pseudomonas putida | |
67.6 | - |
sulfoquinovose | with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication | Pseudomonas putida |