Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Clarias gariepinus | |
ATP | - |
Clupea harengus | |
ATP | - |
Cyprinus carpio | |
ATP | - |
Salmo salar | |
ATP | - |
Salmo trutta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
NADH | pH 7.0, 25°C | Clupea harengus | |
0.16 | - |
(S)-malate | pH 7.5, 25°C | Clupea harengus | |
0.2 | - |
NAD+ | pH 7.5, 25°C | Clupea harengus | |
4.1 | - |
pyruvate | pH 7.0, 25°C | Clupea harengus | |
16.8 | - |
CO2 | pH 7.0, 25°C | Clupea harengus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates | Cyprinus carpio | |
Mn2+ | activates | Salmo salar | |
Mn2+ | activates | Clarias gariepinus | |
Mn2+ | activates | Salmo trutta | |
Mn2+ | activates, Km 0.08 mM in the decarboxylation/oxidation reaction | Clupea harengus | |
Mn2+ | activates, Km is 0.08 mM | Clupea harengus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
4 * 61000, SDS-PAGE | Clupea harengus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Cyprinus carpio | - |
pyruvate + CO2 + NADH | - |
r | |
(S)-malate + NAD+ | Salmo salar | - |
pyruvate + CO2 + NADH | - |
r | |
(S)-malate + NAD+ | Clarias gariepinus | - |
pyruvate + CO2 + NADH | - |
r | |
(S)-malate + NAD+ | Clupea harengus | - |
pyruvate + CO2 + NADH | - |
r | |
(S)-malate + NAD+ | Salmo trutta | - |
pyruvate + CO2 + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clarias gariepinus | - |
- |
- |
Clupea harengus | - |
- |
- |
Clupea harengus | - |
from the Baltic Sea | - |
Cyprinus carpio | - |
- |
- |
Salmo salar | - |
- |
- |
Salmo trutta | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 84.7fold by anion exchange and affinity chromatography, and gel filtration | Clupea harengus |
native NAD-preferring malic enzyme 84.7fold by anion exchange and affinity chromatography followed by gel filtration | Clupea harengus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spermatozoon | high exnzyme activity | Clupea harengus | - |
spermatozoon | two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Cyprinus carpio | - |
spermatozoon | two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Salmo salar | - |
spermatozoon | two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Clarias gariepinus | - |
spermatozoon | two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Clupea harengus | - |
spermatozoon | two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Salmo trutta | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.25 | - |
purified native enzyme, pH 7.0, 25°C, carboxylation reaction | Clupea harengus |
4.25 | - |
purified native enzyme, pH 7.0, 25°C, pyruvate carboxylation | Clupea harengus |
36.09 | - |
purified native enzyme, pH 7.5, 25°C, decarboxylation reaction | Clupea harengus |
36.09 | - |
purified native enzyme, pH 7.5, 25°C, malate decarboxylation | Clupea harengus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Cyprinus carpio | pyruvate + CO2 + NADH | - |
r | |
(S)-malate + NAD+ | - |
Salmo salar | pyruvate + CO2 + NADH | - |
r | |
(S)-malate + NAD+ | - |
Clarias gariepinus | pyruvate + CO2 + NADH | - |
r | |
(S)-malate + NAD+ | - |
Clupea harengus | pyruvate + CO2 + NADH | - |
r | |
(S)-malate + NAD+ | - |
Salmo trutta | pyruvate + CO2 + NADH | - |
r | |
(S)-malate + NAD+ | the decarboxylation reaction is preferred, overview | Clupea harengus | pyruvate + CO2 + NADH | - |
r | |
pyruvate + CO2 + NADH | the rate of carboxylation of pyruvate to malate is lower than for the decarboxylation reaction | Clupea harengus | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, SDS-PAGE | Clupea harengus |
tetramer | 4 * 61000, SDS-PAGE | Clupea harengus |
Synonyms | Comment | Organism |
---|---|---|
NAD-preferring malic enzyme | - |
Cyprinus carpio |
NAD-preferring malic enzyme | - |
Salmo salar |
NAD-preferring malic enzyme | - |
Clarias gariepinus |
NAD-preferring malic enzyme | - |
Clupea harengus |
NAD-preferring malic enzyme | - |
Salmo trutta |
NAD-preferring ME | - |
Clupea harengus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Cyprinus carpio |
25 | - |
assay at | Salmo salar |
25 | - |
assay at | Clarias gariepinus |
25 | - |
assay at | Clupea harengus |
25 | - |
assay at | Salmo trutta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
carboxylation reaction | Clupea harengus |
7 | - |
pyruvate carboxylation | Clupea harengus |
7.5 | - |
assay at | Cyprinus carpio |
7.5 | - |
assay at | Salmo salar |
7.5 | - |
assay at | Clarias gariepinus |
7.5 | - |
assay at | Salmo trutta |
7.5 | - |
decarboxylation reaction | Clupea harengus |
7.5 | - |
malate decarboxylation | Clupea harengus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | decarboxylation reaction, activity range | Clupea harengus |
6 | 9 | activity range, profile overview | Clupea harengus |
6 | 7.5 | carboxylation reaction, activity range | Clupea harengus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cyprinus carpio | |
NAD+ | - |
Salmo salar | |
NAD+ | - |
Clarias gariepinus | |
NAD+ | - |
Clupea harengus | |
NAD+ | - |
Salmo trutta | |
NAD+ | dependent on | Clupea harengus | |
NADH | - |
Cyprinus carpio | |
NADH | - |
Salmo salar | |
NADH | - |
Clarias gariepinus | |
NADH | - |
Clupea harengus | |
NADH | - |
Salmo trutta |
General Information | Comment | Organism |
---|---|---|
metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Cyprinus carpio |
metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Salmo salar |
metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Clarias gariepinus |
metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Clupea harengus |
metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Salmo trutta |