BRENDA - Enzyme Database show
show all sequences of 1.1.1.39

NAD(P)+-malic enzyme mutants of Sinorhizobium sp. strain NGR234, but not Azorhizobium caulinodans ORS571, maintain symbiotic N2 fixation capabilities

Zhang, Y.; Aono, T.; Poole, P.; Finan, T.M.; Appl. Environ. Microbiol. 78, 2803-2812 (2012)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
fumarate
-
Azorhizobium caulinodans
fumarate
-
Sinorhizobium sp.
succinate
-
Azorhizobium caulinodans
succinate
-
Sinorhizobium sp.
Cloned(Commentary)
Commentary
Organism
gene dme or azc3656, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli
Sinorhizobium sp.
Engineering
Amino acid exchange
Commentary
Organism
additional information
azc3656 mutants show about 4fold reduced NAD+-malic enzyme activity
Sinorhizobium sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
acetyl-CoA
-
Azorhizobium caulinodans
acetyl-CoA
-
Sinorhizobium sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.101
-
NAD+
pH 7.8, 30°C, AZC3656
Sinorhizobium sp.
0.59
-
(S)-malate
pH 7.8, 30°C, AZC3656, in presence of 1 mM fumarate
Sinorhizobium sp.
0.64
-
(S)-malate
pH 7.8, 30°C, AZC3656, in presence of 10 mM succinate
Sinorhizobium sp.
2.1
-
NADP+
pH 7.8, 30°C, AZC3656
Sinorhizobium sp.
2.7
-
(S)-malate
pH 7.8, 30°C, AZC3656
Sinorhizobium sp.
27.6
-
(S)-malate
pH 7.8, 30°C, AZC3656, in presence of 0.05 mM acetyl-CoA
Sinorhizobium sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-malate + NAD(P)+
Azorhizobium caulinodans
-
pyruvate + CO2 + NAD(P)H
-
-
?
(S)-malate + NAD+
Sinorhizobium sp.
-
pyruvate + CO2 + NADH
-
-
?
(S)-malate + NAD+
Sinorhizobium sp. NGR234
-
pyruvate + CO2 + NADH
-
-
?
(S)-malate + NADP+
Sinorhizobium sp.
-
pyruvate + CO2 + NADPH
-
-
?
(S)-malate + NADP+
Sinorhizobium sp. NGR234
-
pyruvate + CO2 + NADPH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Azorhizobium caulinodans
-
gene azc3656
-
Sinorhizobium sp.
B6E9W4
strain NGR234; gene azc3656 or dme
-
Sinorhizobium sp. NGR234
B6E9W4
strain NGR234; gene azc3656 or dme
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme AZC3656 from Escherichia coli by nickel affinity chromatography
Sinorhizobium sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.201
-
NAD+-dependent ME activity in the wild-type strain, pH 7.8, 30°C, AZC3656
Sinorhizobium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-malate + NAD(P)+
-
721353
Azorhizobium caulinodans
pyruvate + CO2 + NAD(P)H
-
-
-
?
(S)-malate + NAD+
-
721353
Sinorhizobium sp.
pyruvate + CO2 + NADH
-
-
-
?
(S)-malate + NAD+
-
721353
Sinorhizobium sp. NGR234
pyruvate + CO2 + NADH
-
-
-
?
(S)-malate + NADP+
-
721353
Sinorhizobium sp.
pyruvate + CO2 + NADPH
-
-
-
?
(S)-malate + NADP+
-
721353
Sinorhizobium sp. NGR234
pyruvate + CO2 + NADPH
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Sinorhizobium sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
AZC3656 shows high NAD+-ME activity at pH 7.8, with Michaelis-Menten-like kinetics, at various concentrations of malate, The enzyme exhibits only a very limited positive cooperativity with respect to malate
Sinorhizobium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
preferred cofactor
Azorhizobium caulinodans
NAD+
-
Sinorhizobium sp.
NADP+
-
Azorhizobium caulinodans
NADP+
-
Sinorhizobium sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
fumarate
-
Azorhizobium caulinodans
fumarate
-
Sinorhizobium sp.
succinate
-
Azorhizobium caulinodans
succinate
-
Sinorhizobium sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
gene dme or azc3656, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli
Sinorhizobium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
preferred cofactor
Azorhizobium caulinodans
NAD+
-
Sinorhizobium sp.
NADP+
-
Azorhizobium caulinodans
NADP+
-
Sinorhizobium sp.
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
azc3656 mutants show about 4fold reduced NAD+-malic enzyme activity
Sinorhizobium sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetyl-CoA
-
Azorhizobium caulinodans
acetyl-CoA
-
Sinorhizobium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.101
-
NAD+
pH 7.8, 30°C, AZC3656
Sinorhizobium sp.
0.59
-
(S)-malate
pH 7.8, 30°C, AZC3656, in presence of 1 mM fumarate
Sinorhizobium sp.
0.64
-
(S)-malate
pH 7.8, 30°C, AZC3656, in presence of 10 mM succinate
Sinorhizobium sp.
2.1
-
NADP+
pH 7.8, 30°C, AZC3656
Sinorhizobium sp.
2.7
-
(S)-malate
pH 7.8, 30°C, AZC3656
Sinorhizobium sp.
27.6
-
(S)-malate
pH 7.8, 30°C, AZC3656, in presence of 0.05 mM acetyl-CoA
Sinorhizobium sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-malate + NAD(P)+
Azorhizobium caulinodans
-
pyruvate + CO2 + NAD(P)H
-
-
?
(S)-malate + NAD+
Sinorhizobium sp.
-
pyruvate + CO2 + NADH
-
-
?
(S)-malate + NAD+
Sinorhizobium sp. NGR234
-
pyruvate + CO2 + NADH
-
-
?
(S)-malate + NADP+
Sinorhizobium sp.
-
pyruvate + CO2 + NADPH
-
-
?
(S)-malate + NADP+
Sinorhizobium sp. NGR234
-
pyruvate + CO2 + NADPH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme AZC3656 from Escherichia coli by nickel affinity chromatography
Sinorhizobium sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.201
-
NAD+-dependent ME activity in the wild-type strain, pH 7.8, 30°C, AZC3656
Sinorhizobium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-malate + NAD(P)+
-
721353
Azorhizobium caulinodans
pyruvate + CO2 + NAD(P)H
-
-
-
?
(S)-malate + NAD+
-
721353
Sinorhizobium sp.
pyruvate + CO2 + NADH
-
-
-
?
(S)-malate + NAD+
-
721353
Sinorhizobium sp. NGR234
pyruvate + CO2 + NADH
-
-
-
?
(S)-malate + NADP+
-
721353
Sinorhizobium sp.
pyruvate + CO2 + NADPH
-
-
-
?
(S)-malate + NADP+
-
721353
Sinorhizobium sp. NGR234
pyruvate + CO2 + NADPH
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Sinorhizobium sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
AZC3656 shows high NAD+-ME activity at pH 7.8, with Michaelis-Menten-like kinetics, at various concentrations of malate, The enzyme exhibits only a very limited positive cooperativity with respect to malate
Sinorhizobium sp.
General Information
General Information
Commentary
Organism
malfunction
dme mutants of the broad-host-range Sinorhizobium sp. strain NGR234 form nodules whose level of N2 fixation vary from 27 to 83% (plant dry weight) of the wild-type level, depending on the host plant inoculated. The single dme mutant fixes N2 at reduced rate. A pckA dme double mutant has no N2-fixing activity, PCK is phosphoenolpyruvate carboxykinase. Symbiotic phenotypes of NGR234 and NGR234 dme mutants on different host plants, overview
Sinorhizobium sp.
physiological function
DME is considered an important enzyme for regulating C4-dicarboxylic acid metabolism in N2-fixing bacteroids because its activity is strongly inhibited by acetyl-CoA and stimulated by fumarate and succinate. The NAD+-malic enzyme is required for N2 fixation, and this activity is thought to be required for the anaplerotic synthesis of pyruvate
Azorhizobium caulinodans
physiological function
AZC3656 protein is a NAD+-malic enzyme, i.e. DME, while AZC0119 protein is not a malic enzyme. DME is considered an important enzyme for regulating C4-dicarboxylic acid metabolism in N2-fixing bacteroids because its activity is strongly inhibited by acetyl-CoA and stimulated by fumarate and succinate. The NAD+-malic enzyme is required for N2 fixation, and this activity is thought to be required for the anaplerotic synthesis of pyruvate. But NGR234 bacteroids appear to synthesize pyruvate from TCA cycle intermediates via DME or PCK, phosphoenolpyruvate carboxykinase, pathways, overview
Sinorhizobium sp.
General Information (protein specific)
General Information
Commentary
Organism
malfunction
dme mutants of the broad-host-range Sinorhizobium sp. strain NGR234 form nodules whose level of N2 fixation vary from 27 to 83% (plant dry weight) of the wild-type level, depending on the host plant inoculated. The single dme mutant fixes N2 at reduced rate. A pckA dme double mutant has no N2-fixing activity, PCK is phosphoenolpyruvate carboxykinase. Symbiotic phenotypes of NGR234 and NGR234 dme mutants on different host plants, overview
Sinorhizobium sp.
physiological function
DME is considered an important enzyme for regulating C4-dicarboxylic acid metabolism in N2-fixing bacteroids because its activity is strongly inhibited by acetyl-CoA and stimulated by fumarate and succinate. The NAD+-malic enzyme is required for N2 fixation, and this activity is thought to be required for the anaplerotic synthesis of pyruvate
Azorhizobium caulinodans
physiological function
AZC3656 protein is a NAD+-malic enzyme, i.e. DME, while AZC0119 protein is not a malic enzyme. DME is considered an important enzyme for regulating C4-dicarboxylic acid metabolism in N2-fixing bacteroids because its activity is strongly inhibited by acetyl-CoA and stimulated by fumarate and succinate. The NAD+-malic enzyme is required for N2 fixation, and this activity is thought to be required for the anaplerotic synthesis of pyruvate. But NGR234 bacteroids appear to synthesize pyruvate from TCA cycle intermediates via DME or PCK, phosphoenolpyruvate carboxykinase, pathways, overview
Sinorhizobium sp.
Other publictions for EC 1.1.1.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738272
Niedzwiecka
NAD-preferring malic enzyme: l ...
Clupea harengus
Fish Physiol. Biochem.
43
351-360
2017
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722082
Niedzwiecka
Purification and properties of ...
Clupea harengus
Comp. Biochem. Physiol. B
164
216-220
2013
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5
-
1
1
1
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6
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1
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1
2
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1
1
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2
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5
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1
1
1
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1
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1
2
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2
1
1
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2
2
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721353
Zhang
NAD(P)+-malic enzyme mutants o ...
Azorhizobium caulinodans, Sinorhizobium sp., Sinorhizobium sp. NGR234
Appl. Environ. Microbiol.
78
2803-2812
2012
4
-
1
-
1
-
2
6
-
-
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5
-
13
-
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1
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1
-
5
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1
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1
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4
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4
-
1
4
-
1
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2
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6
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5
-
-
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1
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1
-
5
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
721357
Rodriguez
Impact of malic enzymes on ant ...
Streptomyces coelicolor, Streptomyces coelicolor M145
Appl. Environ. Microbiol.
78
4571-4579
2012
-
-
1
-
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2
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1
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2
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4
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2
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1
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1
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2
2
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721778
Fu
-
The effects of abiotic stresse ...
Triticum aestivum, Triticum aestivum Jinmai 47
Biol. Plant.
55
196-200
2011
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1
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1
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2
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2
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1
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1
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1
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723540
Hsieh
Determinants of nucleotide-bin ...
Ascaris suum
PLoS ONE
6
e25312
2011
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1
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3
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710948
Landete
Requirement of the Lactobacill ...
Lactobacillus casei, Lactobacillus casei BL23 and ATCC 334
Appl. Environ. Microbiol.
76
84-95
2010
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3
3
2
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711147
Tronconi
NAD-malic enzymes of Arabidops ...
Arabidopsis thaliana
Biochem. J.
430
295-303
2010
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1
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1
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7
3
1
1
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2
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4
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1
1
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3
1
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1
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10
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11
10
4
2
2
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3
2
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2
2
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2
4
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711524
Sato
Isolation and properties of ma ...
Rhodopseudomonas palustris, Rhodopseudomonas palustris No. 7
Biosci. Biotechnol. Biochem.
74
75-81
2010
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1
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7
5
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2
4
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1
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1
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3
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2
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2
4
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1
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1
1
6
1
1
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2
4
1
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1
1
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2
2
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4
4
712417
Tronconi
Three different and tissue-spe ...
Arabidopsis thaliana
J. Biol. Chem.
285
11870-11879
2010
8
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1
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1
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5
2
1
1
3
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5
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1
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4
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9
1
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6
3
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1
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21
-
3
3
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2
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9
3
3
1
3
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3
-
12
-
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9
3
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-
6
3
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1
3
-
6
6
713373
Ren
Induction of erythroid differe ...
Homo sapiens
PLoS ONE
5
e12520
2010
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1
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712942
Pongratz
Investigating the roles of mit ...
Rattus norvegicus
Methods Enzymol.
457
425-450
2009
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1
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1
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2
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1
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2
2
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713212
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7
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15
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2
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654720
Karsten
Ascaris suum NAD-malic enzyme ...
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Coleman
Crystal structure of the malic ...
Ascaris suum
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286706
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Characterization of two member ...
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Purification and characterizat ...
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1
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Purification and partial chara ...
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1
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286717
Hrdy
Purification and partial chara ...
Tritrichomonas suis
Parasitology
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Suye
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Purification and properties of ...
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2
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1
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286701
Willeford
pH Effects on the activity and ...
Solanum tuberosum
Plant Physiol.
84
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286702
Willeford
Evidence for a multiple subuni ...
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Willeford
Regulation of the NAD malic en ...
Crassula argentea
Plant Physiol.
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286700
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NAD-malic enzyme from plants ...
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Day
Activation of NAD-linked malic ...
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231
233-242
1984
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Canellas
Kinetic properties of NAD mali ...
Brassica oleracea
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1984
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286707
Wedding
Physical and kinetic propertie ...
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286708
Hatch
Determination of NAD malic enz ...
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Wedding
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Grover
Purification of NAD malic enzy ...
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286711
Canellas
Substrate and metal ion intera ...
Brassica oleracea
Arch. Biochem. Biophys.
199
259-264
1980
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286712
Hoek
Nicotinamide-adenine dinucleot ...
Apis mellifera, Glossina austeni, Glossina longipennis, Glossina morsitans, Glossina pallidipes, Heliocarpus sp., Odontotermes sp., Periplaneta americana, Sarcophaga sp., Schistocerca gregaria, Spodoptera exempta
Biochem. J.
160
253-262
1976
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2
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2
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11
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12
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12
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12
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1
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12
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1
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286713
Hatch
Properties of leaf NAD malic e ...
Amaranthus edulis, Atriplex spongiosa, Panicum miliaceum
Arch. Biochem. Biophys.
165
188-200
1974
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1
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4
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286714
Mounib
NAD- and NADP-malic enzymes in ...
Bos taurus, Gadidae, Homo sapiens, salmon
FEBS Lett.
48
79-84
1974
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286715
Lin
Malic enzymes of rabbit heart ...
Oryctolagus cuniculus
J. Biol. Chem.
249
3867-3875
1974
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2
8
1
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286716
Eyzaguirre
Two malic enzymes in Pseudomon ...
Pseudomonas aeruginosa
J. Bacteriol.
116
215-221
1973
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