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Literature summary for 1.1.1.39 extracted from
- Investigating the roles of mitochondrial and cytosolic malic enzyme in insulin secretion (2009), Methods Enzymol., 457, 425-450.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ME2, quantitative real time PCR expression analysis | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | siRNA knockout of ME2 in INS-1 832/13 beta-cells, siRNA knockdown and isotopic labeling strategies, method optimization, overview | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics analysis of isozymes ME2 and ME3 | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | isozymes ME2 and ME3 | Rattus norvegicus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Rattus norvegicus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | Rattus norvegicus | - |
pyruvate + NADH + H+ + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
isozyme ME2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme partially by preparation of mitochondria, method, overview | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| INS-1 832/13 cell | insulinoma cells, isozyme ME2 | Rattus norvegicus | - |
| pancreatic beta cell | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | - |
Rattus norvegicus | pyruvate + NADH + H+ + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| malic enzyme 2 | - |
Rattus norvegicus |
| ME2 | - |
Rattus norvegicus |
| mitochondrial malic enzyme | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Rattus norvegicus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the citrate-malate-pyruvate cycle serves to regenerate NAD+ and maintain glycolytic flux. Pyruvate cycles all lead to the exchange of reducing equivalents from mitochondrial NADH to cytosolic NADPH. Malic enzyme is integral to the coupling of metabolism with insulin secretion | Rattus norvegicus |
| physiological function | siRNA knockdown and isotopic labeling strategies to evaluate the role of cytosolic and mitochondrial isozymes of malic enzyme in facilitating malate-pyruvate cycling in the context of fuel-stimulated insulin secretion, overview | Rattus norvegicus |
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Release 2023.1 (January 2023)
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