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Literature summary for 1.1.1.39 extracted from

  • Tronconi, M.A.; Maurino, V.G.; Andreo, C.S.; Drincovich, M.F.
    Three different and tissue-specific NAD-malic enzymes generated by alternative subunit association in Arabidopsis thaliana (2010), J. Biol. Chem., 285, 11870-11879.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-oxoglutarate
-
Arabidopsis thaliana
acetyl-CoA
-
Arabidopsis thaliana
CoA activation kinetics, overview Arabidopsis thaliana
D-fructose 1,6-bisphosphate
-
Arabidopsis thaliana
fumarate
-
Arabidopsis thaliana
additional information no activation by CoA and acetyl-CoA, poor activation by ATP and AMP Arabidopsis thaliana
additional information no activation by oxaloacetate, poor activation by ATP and AMP Arabidopsis thaliana
additional information poor activation by ATP and AMP Arabidopsis thaliana
oxaloacetate
-
Arabidopsis thaliana
phosphoenolpyruvate
-
Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged NAD-ME1 and of mutants NADME1q and NAD-ME2q in Escherichia coli strain BL21(DE3) Arabidopsis thaliana
expression of His-tagged NAD-ME2 and mutants NADME1q and NAD-ME2q in Escherichia coli strain BL21(DE3) Arabidopsis thaliana
expression of His-tagged NAD-MEH in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of two chimeras NADME1q and NAD-ME2q by interchanging the first 176 amino residues between NAD-ME1 and -2, altered regulation in comparison to the wild-type enzymes, overview Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic analysis and comparison of the different isozymes MAD-ME1, NAD-ME2, and NAD-MEH, and of mutants NADME1q and NAD-ME2q, overview Arabidopsis thaliana
0.5
-
NAD+ pH 6.4, temperature not specified in the publication, NAD-ME1 Arabidopsis thaliana
0.5
-
NAD+ pH 6.6, temperature not specified in the publication, NAD-ME2 Arabidopsis thaliana
0.55
-
NAD+ pH 6.5, temperature not specified in the publication, NAD-MEH Arabidopsis thaliana
2.7
-
(S)-malate pH 6.5, temperature not specified in the publication, NAD-MEH Arabidopsis thaliana
3
-
(S)-malate pH 6.4, temperature not specified in the publication, NAD-ME1 Arabidopsis thaliana
3
-
(S)-malate pH 6.6, temperature not specified in the publication, NAD-ME2 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Arabidopsis thaliana 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000
-
NAD-MEH, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Arabidopsis thaliana
-
pyruvate + NADH + H+ + CO2
-
ir

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Q8L7K9 isozyme NAD-ME2
-
Arabidopsis thaliana Q9SIU0 isozyme NAD-ME1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged NAD-ME1 and mutants NADME1q and NAD-ME2q from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Arabidopsis thaliana
recombinant His-tagged NAD-ME2 and mutants NADME1q and NAD-ME2q from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filteration Arabidopsis thaliana
recombinant His-tagged NAD-MEH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Arabidopsis thaliana
-
flower NAD-ME1and -2 subunits show a distinct patterns of accumulation in the separate components of the floral organ Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
sepal NAD-MEH and NADME1 act in concert in this tissue Arabidopsis thaliana
-
sepal the NAD-ME1 subunit is present at a slightly higher proportion than the NAD-ME2 subunit, and thus, NAD-MEH and NADME1 act in concert in this tissue Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Arabidopsis thaliana pyruvate + NADH + H+ + CO2
-
ir
(S)-malate + NAD+ very low activity in the reverse reaction in vitro Arabidopsis thaliana pyruvate + NADH + H+ + CO2
-
r
additional information NAD-ME1 does not perform decarboxylation of oxaloacetate Arabidopsis thaliana ?
-
?
additional information NAD-ME2 does not perform decarboxylation of oxaloacetate Arabidopsis thaliana ?
-
?
additional information NAD-MEH does not perform decarboxylation of oxaloacetate Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
More isozyme NAD-ME1 primary structure and domain analysis, overview Arabidopsis thaliana
More isozyme NAD-ME2 primary structure and domain analysis, overview Arabidopsis thaliana
More isozyme NAD-MEH primary structure and domain analysis, overview Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
NAD-malic enzyme
-
Arabidopsis thaliana
NAD-ME1
-
Arabidopsis thaliana
NAD-ME2
-
Arabidopsis thaliana
NAD-MEH
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
31.1
-
NAD+ pH 6.4, temperature not specified in the publication, NAD-ME1 Arabidopsis thaliana
31.1
-
(S)-malate pH 6.4, temperature not specified in the publication, NAD-ME1 Arabidopsis thaliana
39
-
NAD+ pH 6.5, temperature not specified in the publication, NAD-MEH Arabidopsis thaliana
39
-
(S)-malate pH 6.5, temperature not specified in the publication, NAD-MEH Arabidopsis thaliana
44.1
-
NAD+ pH 6.6, temperature not specified in the publication, NAD-ME2 Arabidopsis thaliana
44.1
-
(S)-malate pH 6.6, temperature not specified in the publication, NAD-ME2 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
NAD-ME1 Arabidopsis thaliana
6.5
-
NAD-MEH Arabidopsis thaliana
6.6
-
NAD-ME2 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Arabidopsis thaliana

General Information

General Information Comment Organism
additional information identification of specific domains of the primary structure, which are involved in the differential allosteric regulation. Different properties of NAD-ME1, -2, and -H, mitochondrial NAD-ME activity may be regulated by varying native association in vivo, rendering enzymatic entities with distinct allosteric regulation to fulfill specific roles, overview Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10.3
-
(S)-malate pH 6.4, temperature not specified in the publication, NAD-ME1 Arabidopsis thaliana
14.2
-
(S)-malate pH 6.5, temperature not specified in the publication, NAD-MEH Arabidopsis thaliana
14.7
-
(S)-malate pH 6.6, temperature not specified in the publication, NAD-ME2 Arabidopsis thaliana
60.2
-
NAD+ pH 6.4, temperature not specified in the publication, NAD-ME1 Arabidopsis thaliana
67
-
NAD+ pH 6.5, temperature not specified in the publication, NAD-MEH Arabidopsis thaliana
88.2
-
NAD+ pH 6.6, temperature not specified in the publication, NAD-ME2 Arabidopsis thaliana