BRENDA - Enzyme Database show
show all sequences of 1.1.1.39

Three different and tissue-specific NAD-malic enzymes generated by alternative subunit association in Arabidopsis thaliana

Tronconi, M.A.; Maurino, V.G.; Andreo, C.S.; Drincovich, M.F.; J. Biol. Chem. 285, 11870-11879 (2010)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-oxoglutarate
-
Arabidopsis thaliana
acetyl-CoA
;
Arabidopsis thaliana
CoA
activation kinetics, overview; activation kinetics, overview
Arabidopsis thaliana
D-fructose 1,6-bisphosphate
-
Arabidopsis thaliana
fumarate
-
Arabidopsis thaliana
additional information
no activation by CoA and acetyl-CoA, poor activation by ATP and AMP; no activation by oxaloacetate, poor activation by ATP and AMP; poor activation by ATP and AMP
Arabidopsis thaliana
oxaloacetate
;
Arabidopsis thaliana
phosphoenolpyruvate
-
Arabidopsis thaliana
Cloned(Commentary)
Commentary
Organism
expression of His-tagged NAD-ME1 and of mutants NADME1q and NAD-ME2q in Escherichia coli strain BL21(DE3); expression of His-tagged NAD-ME2 and mutants NADME1q and NAD-ME2q in Escherichia coli strain BL21(DE3); expression of His-tagged NAD-MEH in Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of two chimeras NADME1q and NAD-ME2q by interchanging the first 176 amino residues between NAD-ME1 and -2, altered regulation in comparison to the wild-type enzymes, overview; construction of two chimeras NADME1q and NAD-ME2q by interchanging the first 176 amino residues between NAD-ME1 and -2, altered regulation in comparison to the wild-type enzymes, overview
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic analysis and comparison of the different isozymes MAD-ME1, NAD-ME2, and NAD-MEH, and of mutants NADME1q and NAD-ME2q, overview; kinetic analysis and comparison of the different isozymes MAD-ME1, NAD-ME2, and NAD-MEH, and of mutants NADME1q and NAD-ME2q, overview; kinetic analysis and comparison of the different isozymes MAD-ME1, NAD-ME2, and NAD-MEH, and of mutants NADME1q and NAD-ME2q, overview
Arabidopsis thaliana
0.5
-
NAD+
pH 6.4, temperature not specified in the publication, NAD-ME1; pH 6.6, temperature not specified in the publication, NAD-ME2
Arabidopsis thaliana
0.55
-
NAD+
pH 6.5, temperature not specified in the publication, NAD-MEH
Arabidopsis thaliana
2.7
-
(S)-malate
pH 6.5, temperature not specified in the publication, NAD-MEH
Arabidopsis thaliana
3
-
(S)-malate
pH 6.4, temperature not specified in the publication, NAD-ME1; pH 6.6, temperature not specified in the publication, NAD-ME2
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
;
Arabidopsis thaliana
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
activates; activates; activates
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
125000
-
NAD-MEH, gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-malate + NAD+
Arabidopsis thaliana
-
pyruvate + NADH + H+ + CO2
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana
Q8L7K9
isozyme NAD-ME2
-
Arabidopsis thaliana
Q9T0H6
isozyme NAD-ME1
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged NAD-ME1 and mutants NADME1q and NAD-ME2q from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration; recombinant His-tagged NAD-ME2 and mutants NADME1q and NAD-ME2q from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filteration; recombinant His-tagged NAD-MEH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
; NAD-ME1and -2 subunits show a distinct patterns of accumulation in the separate components of the floral organ; NAD-ME1and -2 subunits show a distinct patterns of accumulation in the separate components of the floral organ
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
sepal
NAD-MEH and NADME1 act in concert in this tissue; the NAD-ME1 subunit is present at a slightly higher proportion than the NAD-ME2 subunit, and thus, NAD-MEH and NADME1 act in concert in this tissue; the NAD-ME1 subunit is present at a slightly higher proportion than the NAD-ME2 subunit, and thus, NAD-MEH and NADME1 act in concert in this tissue
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-malate + NAD+
-
712417
Arabidopsis thaliana
pyruvate + NADH + H+ + CO2
-
-
-
ir
(S)-malate + NAD+
very low activity in the reverse reaction in vitro
712417
Arabidopsis thaliana
pyruvate + NADH + H+ + CO2
-
-
-
r
additional information
NAD-ME1 does not perform decarboxylation of oxaloacetate
712417
Arabidopsis thaliana
?
-
-
-
-
additional information
NAD-ME2 does not perform decarboxylation of oxaloacetate
712417
Arabidopsis thaliana
?
-
-
-
-
additional information
NAD-MEH does not perform decarboxylation of oxaloacetate
712417
Arabidopsis thaliana
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
More
isozyme NAD-ME1 primary structure and domain analysis, overview; isozyme NAD-ME2 primary structure and domain analysis, overview; isozyme NAD-MEH primary structure and domain analysis, overview
Arabidopsis thaliana
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
31.1
-
NAD+
pH 6.4, temperature not specified in the publication, NAD-ME1
Arabidopsis thaliana
31.1
-
(S)-malate
pH 6.4, temperature not specified in the publication, NAD-ME1
Arabidopsis thaliana
39
-
NAD+
pH 6.5, temperature not specified in the publication, NAD-MEH
Arabidopsis thaliana
39
-
(S)-malate
pH 6.5, temperature not specified in the publication, NAD-MEH
Arabidopsis thaliana
44.1
-
NAD+
pH 6.6, temperature not specified in the publication, NAD-ME2
Arabidopsis thaliana
44.1
-
(S)-malate
pH 6.6, temperature not specified in the publication, NAD-ME2
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
NAD-ME1
Arabidopsis thaliana
6.5
-
NAD-MEH
Arabidopsis thaliana
6.6
-
NAD-ME2
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Arabidopsis thaliana
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-oxoglutarate
-
Arabidopsis thaliana
acetyl-CoA
-
Arabidopsis thaliana
CoA
activation kinetics, overview
Arabidopsis thaliana
D-fructose 1,6-bisphosphate
-
Arabidopsis thaliana
fumarate
-
Arabidopsis thaliana
additional information
no activation by oxaloacetate, poor activation by ATP and AMP
Arabidopsis thaliana
additional information
no activation by CoA and acetyl-CoA, poor activation by ATP and AMP
Arabidopsis thaliana
additional information
poor activation by ATP and AMP
Arabidopsis thaliana
oxaloacetate
-
Arabidopsis thaliana
phosphoenolpyruvate
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of His-tagged NAD-ME1 and of mutants NADME1q and NAD-ME2q in Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
expression of His-tagged NAD-ME2 and mutants NADME1q and NAD-ME2q in Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
expression of His-tagged NAD-MEH in Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of two chimeras NADME1q and NAD-ME2q by interchanging the first 176 amino residues between NAD-ME1 and -2, altered regulation in comparison to the wild-type enzymes, overview
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic analysis and comparison of the different isozymes MAD-ME1, NAD-ME2, and NAD-MEH, and of mutants NADME1q and NAD-ME2q, overview
Arabidopsis thaliana
0.5
-
NAD+
pH 6.6, temperature not specified in the publication, NAD-ME2
Arabidopsis thaliana
0.5
-
NAD+
pH 6.4, temperature not specified in the publication, NAD-ME1
Arabidopsis thaliana
0.55
-
NAD+
pH 6.5, temperature not specified in the publication, NAD-MEH
Arabidopsis thaliana
2.7
-
(S)-malate
pH 6.5, temperature not specified in the publication, NAD-MEH
Arabidopsis thaliana
3
-
(S)-malate
pH 6.6, temperature not specified in the publication, NAD-ME2
Arabidopsis thaliana
3
-
(S)-malate
pH 6.4, temperature not specified in the publication, NAD-ME1
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Arabidopsis thaliana
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
activates
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
125000
-
NAD-MEH, gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-malate + NAD+
Arabidopsis thaliana
-
pyruvate + NADH + H+ + CO2
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged NAD-ME1 and mutants NADME1q and NAD-ME2q from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Arabidopsis thaliana
recombinant His-tagged NAD-ME2 and mutants NADME1q and NAD-ME2q from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filteration
Arabidopsis thaliana
recombinant His-tagged NAD-MEH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
NAD-ME1and -2 subunits show a distinct patterns of accumulation in the separate components of the floral organ
Arabidopsis thaliana
-
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
sepal
the NAD-ME1 subunit is present at a slightly higher proportion than the NAD-ME2 subunit, and thus, NAD-MEH and NADME1 act in concert in this tissue
Arabidopsis thaliana
-
sepal
NAD-MEH and NADME1 act in concert in this tissue
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-malate + NAD+
-
712417
Arabidopsis thaliana
pyruvate + NADH + H+ + CO2
-
-
-
ir
(S)-malate + NAD+
very low activity in the reverse reaction in vitro
712417
Arabidopsis thaliana
pyruvate + NADH + H+ + CO2
-
-
-
r
additional information
NAD-ME1 does not perform decarboxylation of oxaloacetate
712417
Arabidopsis thaliana
?
-
-
-
-
additional information
NAD-ME2 does not perform decarboxylation of oxaloacetate
712417
Arabidopsis thaliana
?
-
-
-
-
additional information
NAD-MEH does not perform decarboxylation of oxaloacetate
712417
Arabidopsis thaliana
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
More
isozyme NAD-ME2 primary structure and domain analysis, overview
Arabidopsis thaliana
More
isozyme NAD-ME1 primary structure and domain analysis, overview
Arabidopsis thaliana
More
isozyme NAD-MEH primary structure and domain analysis, overview
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
31.1
-
NAD+
pH 6.4, temperature not specified in the publication, NAD-ME1
Arabidopsis thaliana
31.1
-
(S)-malate
pH 6.4, temperature not specified in the publication, NAD-ME1
Arabidopsis thaliana
39
-
NAD+
pH 6.5, temperature not specified in the publication, NAD-MEH
Arabidopsis thaliana
39
-
(S)-malate
pH 6.5, temperature not specified in the publication, NAD-MEH
Arabidopsis thaliana
44.1
-
NAD+
pH 6.6, temperature not specified in the publication, NAD-ME2
Arabidopsis thaliana
44.1
-
(S)-malate
pH 6.6, temperature not specified in the publication, NAD-ME2
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
NAD-ME1
Arabidopsis thaliana
6.5
-
NAD-MEH
Arabidopsis thaliana
6.6
-
NAD-ME2
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
additional information
identification of specific domains of the primary structure, which are involved in the differential allosteric regulation. Different properties of NAD-ME1, -2, and -H, mitochondrial NAD-ME activity may be regulated by varying native association in vivo, rendering enzymatic entities with distinct allosteric regulation to fulfill specific roles, overview; identification of specific domains of the primary structure, which are involved in the differential allosteric regulation. Different properties of NAD-ME1, -2, and -H, mitochondrial NAD-ME activity may be regulated by varying native association in vivo, rendering enzymatic entities with distinct allosteric regulation to fulfill specific roles, overview; identification of specific domains of the primary structure, which are involved in the differential allosteric regulation. Different properties of NAD-ME1, -2, and -H, mitochondrial NAD-ME activity may be regulated by varying native association in vivo, rendering enzymatic entities with distinct allosteric regulation to fulfill specific roles, overview
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
additional information
identification of specific domains of the primary structure, which are involved in the differential allosteric regulation. Different properties of NAD-ME1, -2, and -H, mitochondrial NAD-ME activity may be regulated by varying native association in vivo, rendering enzymatic entities with distinct allosteric regulation to fulfill specific roles, overview
Arabidopsis thaliana
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
10.3
-
(S)-malate
pH 6.4, temperature not specified in the publication, NAD-ME1
Arabidopsis thaliana
14.2
-
(S)-malate
pH 6.5, temperature not specified in the publication, NAD-MEH
Arabidopsis thaliana
14.7
-
(S)-malate
pH 6.6, temperature not specified in the publication, NAD-ME2
Arabidopsis thaliana
60.2
-
NAD+
pH 6.4, temperature not specified in the publication, NAD-ME1
Arabidopsis thaliana
67
-
NAD+
pH 6.5, temperature not specified in the publication, NAD-MEH
Arabidopsis thaliana
88.2
-
NAD+
pH 6.6, temperature not specified in the publication, NAD-ME2
Arabidopsis thaliana
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
10.3
-
(S)-malate
pH 6.4, temperature not specified in the publication, NAD-ME1
Arabidopsis thaliana
14.2
-
(S)-malate
pH 6.5, temperature not specified in the publication, NAD-MEH
Arabidopsis thaliana
14.7
-
(S)-malate
pH 6.6, temperature not specified in the publication, NAD-ME2
Arabidopsis thaliana
60.2
-
NAD+
pH 6.4, temperature not specified in the publication, NAD-ME1
Arabidopsis thaliana
67
-
NAD+
pH 6.5, temperature not specified in the publication, NAD-MEH
Arabidopsis thaliana
88.2
-
NAD+
pH 6.6, temperature not specified in the publication, NAD-ME2
Arabidopsis thaliana
Other publictions for EC 1.1.1.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738272
Niedzwiecka
NAD-preferring malic enzyme: l ...
Clupea harengus
Fish Physiol. Biochem.
43
351-360
2017
-
-
-
-
-
-
1
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
1
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
722082
Niedzwiecka
Purification and properties of ...
Clupea harengus
Comp. Biochem. Physiol. B
164
216-220
2013
-
-
-
-
-
-
-
5
-
1
1
1
-
6
-
-
1
-
-
1
2
-
2
1
1
-
-
-
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
5
-
1
1
1
-
-
-
1
-
1
2
-
2
1
1
-
-
-
2
2
-
-
-
-
-
-
-
-
721353
Zhang
NAD(P)+-malic enzyme mutants o ...
Azorhizobium caulinodans, Sinorhizobium sp., Sinorhizobium sp. NGR234
Appl. Environ. Microbiol.
78
2803-2812
2012
4
-
1
-
1
-
2
6
-
-
-
5
-
13
-
-
1
-
-
-
1
-
5
-
1
-
-
-
1
-
-
4
-
-
-
4
-
1
4
-
1
-
-
2
-
6
-
-
-
5
-
-
-
1
-
-
1
-
5
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
721357
Rodriguez
Impact of malic enzymes on ant ...
Streptomyces coelicolor, Streptomyces coelicolor M145
Appl. Environ. Microbiol.
78
4571-4579
2012
-
-
1
-
-
-
-
2
-
1
-
2
-
4
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
721778
Fu
-
The effects of abiotic stresse ...
Triticum aestivum, Triticum aestivum Jinmai 47
Biol. Plant.
55
196-200
2011
-
-
1
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
723540
Hsieh
Determinants of nucleotide-bin ...
Ascaris suum
PLoS ONE
6
e25312
2011
-
-
-
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710948
Landete
Requirement of the Lactobacill ...
Lactobacillus casei, Lactobacillus casei BL23 and ATCC 334
Appl. Environ. Microbiol.
76
84-95
2010
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
3
3
2
-
-
711147
Tronconi
NAD-malic enzymes of Arabidops ...
Arabidopsis thaliana
Biochem. J.
430
295-303
2010
-
-
1
-
1
-
7
3
1
1
-
2
-
4
-
-
1
1
-
-
-
-
3
1
-
-
-
2
1
-
-
1
10
-
-
-
-
2
2
-
2
-
-
11
10
4
2
2
-
2
-
-
-
2
-
-
-
-
3
2
-
-
-
2
2
-
-
-
-
2
4
-
-
-
711524
Sato
Isolation and properties of ma ...
Rhodopseudomonas palustris, Rhodopseudomonas palustris No. 7
Biosci. Biotechnol. Biochem.
74
75-81
2010
-
-
1
-
-
-
7
5
-
6
2
4
-
2
-
-
1
-
-
-
1
1
6
1
1
-
2
4
1
-
1
3
2
1
-
-
-
1
3
-
-
-
-
7
2
5
-
6
2
4
-
-
-
1
-
-
1
1
6
1
1
-
2
4
1
-
1
1
-
2
2
-
4
4
712417
Tronconi
Three different and tissue-spe ...
Arabidopsis thaliana
J. Biol. Chem.
285
11870-11879
2010
8
-
1
-
1
-
-
5
2
1
1
3
-
5
-
-
1
-
-
4
-
-
9
1
-
-
-
6
3
-
-
1
-
-
-
21
-
3
3
-
2
-
-
-
-
9
3
3
1
3
-
-
-
3
-
12
-
-
9
3
-
-
-
6
3
-
-
-
-
1
3
-
6
6
713373
Ren
Induction of erythroid differe ...
Homo sapiens
PLoS ONE
5
e12520
2010
-
1
-
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712942
Pongratz
Investigating the roles of mit ...
Rattus norvegicus
Methods Enzymol.
457
425-450
2009
-
-
1
-
1
-
-
1
1
1
-
1
-
1
-
-
1
-
-
2
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
1
1
1
-
1
-
-
-
1
-
2
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
713212
Rzepka
-
Effect of abiotic stress facto ...
Mnium undulatum, Pilosella officinarum, Polytrichum commune, Polytrichum piliferum
Photosynthetica
47
141-145
2009
-
-
-
-
-
-
4
-
8
4
-
8
-
4
-
-
-
-
-
4
4
-
8
-
4
-
-
-
4
-
-
8
-
-
-
-
-
-
8
-
-
-
-
4
-
-
8
4
-
8
-
-
-
-
-
4
4
-
8
-
4
-
-
-
4
-
-
-
-
-
-
-
-
-
685215
Aktas
Proper positioning of the nico ...
Ascaris suum
Biochemistry
47
2539-2546
2008
-
-
-
-
7
-
-
15
1
1
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
7
-
-
-
-
15
1
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
685678
Ohno
Reverse reaction of malic enzy ...
Brevundimonas diminuta, Brevundimonas diminuta IFO 13182
Biosci. Biotechnol. Biochem.
72
1278-1282
2008
-
1
-
-
1
-
-
-
-
1
-
2
-
2
-
-
1
-
-
-
1
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
1
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
689604
Tronconi
Arabidopsis NAD-malic enzyme f ...
Arabidopsis thaliana
Plant Physiol.
146
1540-1552
2008
-
-
1
-
-
-
-
2
1
-
4
2
-
2
-
-
-
-
-
2
-
-
4
2
-
-
-
2
2
-
-
2
-
-
-
-
-
2
4
-
-
-
-
-
-
4
2
-
4
2
-
-
-
-
-
4
-
-
4
4
-
-
-
2
2
-
-
-
-
-
-
-
-
-
685123
Karsten
Multiple roles of arginine 181 ...
Ascaris suum
Biochemistry
46
14578-14588
2007
-
-
-
-
2
-
1
8
-
2
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
3
2
-
-
-
-
-
3
-
2
-
-
1
2
8
-
2
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
685622
Chang
Metal ions stabilize a dimeric ...
Homo sapiens
Biophys. J.
93
3977-3988
2007
-
-
-
-
-
-
1
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
687568
Pongratz
Cytosolic and mitochondrial ma ...
Rattus norvegicus
J. Biol. Chem.
282
200-207
2007
-
-
1
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
3
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669132
Mitsch
Malic Enzyme Cofactor and Doma ...
Sinorhizobium meliloti
J. Bacteriol.
189
160-168
2006
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656983
Lara
Induction of a crassulacean ac ...
Portulaca oleracea
Plant Cell Physiol.
45
618-626
2004
-
-
-
-
-
-
1
-
3
1
-
1
-
1
-
-
-
-
-
2
3
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
3
1
-
1
-
-
-
-
-
2
3
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657321
Kuo
Metal-induced reversible struc ...
Homo sapiens
Proteins
54
404-411
2004
-
-
-
-
-
-
1
-
2
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
2
3
-
-
-
-
-
2
-
-
-
-
1
3
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
654720
Karsten
Ascaris suum NAD-malic enzyme ...
Ascaris suum
Biochemistry
42
9712-9721
2003
2
-
1
-
2
-
1
1
1
3
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
2
1
-
-
2
-
1
2
-
2
-
-
1
1
1
1
3
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
656163
Rao
Crystallographic studies on As ...
Ascaris suum
J. Biol. Chem.
278
38051-38058
2003
-
-
-
1
-
-
1
-
1
1
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657362
Tao
Crystal structures of substrat ...
Homo sapiens
Structure
11
1141-1150
2003
1
-
-
1
-
-
-
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
4
1
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654623
Liu
Alternative substrates for mal ...
Ascaris suum
Biochemistry
41
12200-12203
2002
-
-
-
-
-
-
1
6
-
1
-
-
-
1
-
-
-
1
-
-
-
-
6
-
1
-
-
-
1
-
-
1
3
-
-
-
-
-
1
-
-
-
-
1
3
6
-
1
-
-
-
-
-
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
654651
Coleman
Crystal structure of the malic ...
Ascaris suum
Biochemistry
41
6928-6938
2002
-
-
-
1
-
-
-
-
1
-
-
1
-
1
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286706
Voegele
Characterization of two member ...
Sinorhizobium meliloti
Biochim. Biophys. Acta
1432
275-285
1999
6
-
-
-
-
-
11
2
-
-
1
-
-
2
-
-
1
-
-
-
1
2
2
1
-
-
-
-
-
-
-
2
-
-
-
6
-
-
2
-
-
-
-
11
-
2
-
-
1
-
-
-
-
1
-
-
1
2
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286720
Chen
Purification and characterizat ...
Bradyrhizobium japonicum
Appl. Environ. Microbiol.
64
4073-4075
1998
10
-
-
-
-
-
11
-
-
2
2
-
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
10
-
-
1
-
-
-
-
11
-
-
-
2
2
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286719
Farias de Aragao
-
Purification and partial chara ...
Vigna unguiculata
Plant Physiol. Biochem.
34
363-368
1996
-
-
-
-
-
-
-
1
-
1
3
-
-
1
-
-
1
1
-
1
1
-
1
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
3
-
-
-
-
1
-
1
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286717
Hrdy
Purification and partial chara ...
Tritrichomonas suis
Parasitology
107
379-385
1993
-
-
-
-
-
-
-
4
2
2
1
-
-
2
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
2
2
1
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286718
Suye
-
Purification and properties of ...
Brevundimonas diminuta
J. Ferment. Bioeng.
73
343-347
1992
-
-
-
-
-
-
7
2
-
2
2
-
-
1
-
-
1
-
-
-
2
-
2
1
1
1
2
-
1
1
1
2
-
-
-
-
-
-
2
-
-
-
-
7
-
2
-
2
2
-
-
-
-
1
-
-
2
-
2
1
1
1
2
-
1
1
1
-
-
-
-
-
-
-
286701
Willeford
pH Effects on the activity and ...
Solanum tuberosum
Plant Physiol.
84
1084-1087
1987
-
-
-
-
-
1
1
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
1
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
286702
Willeford
Evidence for a multiple subuni ...
Crassula argentea, Solanum tuberosum
J. Biol. Chem.
262
8423-8429
1987
-
-
-
-
-
-
-
-
-
-
2
-
-
6
-
-
1
-
-
-
1
-
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286703
Willeford
Regulation of the NAD malic en ...
Crassula argentea
Plant Physiol.
80
792-795
1986
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286700
Artus
-
NAD-malic enzyme from plants ...
Atriplex spongiosa, Brassica oleracea, Crassula argentea, Solanum tuberosum
FEBS Lett.
182
225-233
1985
-
-
-
-
-
-
-
-
-
-
7
-
-
4
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286704
Day
Activation of NAD-linked malic ...
Brassica oleracea, Solanum tuberosum
Arch. Biochem. Biophys.
231
233-242
1984
2
-
-
-
-
-
2
-
2
-
-
2
-
2
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
2
-
-
-
-
2
-
-
2
-
-
2
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286705
Canellas
Kinetic properties of NAD mali ...
Brassica oleracea
Arch. Biochem. Biophys.
229
414-425
1984
-
-
-
-
-
-
2
3
-
3
-
-
-
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
3
-
3
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286707
Wedding
Physical and kinetic propertie ...
Crassula argentea
Plant Physiol.
72
1021-1028
1983
6
-
-
-
-
-
5
7
-
2
4
-
-
1
-
-
1
1
-
1
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
6
-
-
2
-
-
-
-
5
-
7
-
2
4
-
-
-
-
1
-
1
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286708
Hatch
Determination of NAD malic enz ...
Amaranthus retroflexus
Plant Physiol.
69
483-491
1982
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286709
Wedding
Slow transients in the activit ...
Crassula argentea
Plant Physiol.
68
1416-1423
1981
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
286710
Grover
Purification of NAD malic enzy ...
Solanum tuberosum
Arch. Biochem. Biophys.
209
396-407
1981
-
-
-
-
-
-
-
8
-
2
3
-
-
1
-
-
1
-
-
1
1
-
2
1
-
-
-
1
3
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
8
-
2
3
-
-
-
-
1
-
1
1
-
2
1
-
-
-
1
3
-
-
-
-
-
-
-
-
-
286711
Canellas
Substrate and metal ion intera ...
Brassica oleracea
Arch. Biochem. Biophys.
199
259-264
1980
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286712
Hoek
Nicotinamide-adenine dinucleot ...
Apis mellifera, Glossina austeni, Glossina longipennis, Glossina morsitans, Glossina pallidipes, Heliocarpus sp., Odontotermes sp., Periplaneta americana, Sarcophaga sp., Schistocerca gregaria, Spodoptera exempta
Biochem. J.
160
253-262
1976
-
-
-
-
-
-
-
2
2
2
-
-
-
11
-
-
-
-
-
1
1
-
12
-
-
-
-
-
1
1
-
12
-
-
-
-
-
-
12
-
-
-
-
-
-
2
2
2
-
-
-
-
-
-
-
1
1
-
12
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
286713
Hatch
Properties of leaf NAD malic e ...
Amaranthus edulis, Atriplex spongiosa, Panicum miliaceum
Arch. Biochem. Biophys.
165
188-200
1974
4
-
-
-
-
1
4
1
-
6
1
-
-
3
-
-
1
-
-
3
-
1
4
-
-
-
-
-
2
-
-
4
-
-
-
4
-
-
4
-
-
1
-
4
-
1
-
6
1
-
-
-
-
1
-
3
-
1
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
286714
Mounib
NAD- and NADP-malic enzymes in ...
Bos taurus, Gadidae, Homo sapiens, salmon
FEBS Lett.
48
79-84
1974
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286715
Lin
Malic enzymes of rabbit heart ...
Oryctolagus cuniculus
J. Biol. Chem.
249
3867-3875
1974
-
-
-
-
-
-
2
8
1
2
1
-
-
1
-
-
1
-
-
1
-
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
8
1
2
1
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286716
Eyzaguirre
Two malic enzymes in Pseudomon ...
Pseudomonas aeruginosa
J. Bacteriol.
116
215-221
1973
-
-
-
-
-
-
-
1
-
2
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-