BRENDA - Enzyme Database show
show all sequences of 1.1.1.39

Arabidopsis NAD-malic enzyme functions as a homodimer and heterodimer and has a major impact on nocturnal metabolism

Tronconi, M.A.; Fahnenstich, H.; Gerrard Weehler, M.C.; Andreo, C.S.; Fluegge, U.I.; Drincovich, M.F.; Maurino, V.G.; Plant Physiol. 146, 1540-1552 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene AtNAD-ME1, DNA and amino acid sequence determination and analysis, phylogenetic tree; gene AtNAD-ME2, DNA and amino acid sequence determination and analysis, phylogenetic tree
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.5
-
NAD+
pH 7.4, 30°C, isozyme NAD-ME1; pH 7.4, 30°C, isozyme NAD-ME2
Arabidopsis thaliana
3
-
(S)-malate
pH 7.4, 30°C, isozyme NAD-ME1; pH 7.4, 30°C, isozyme NAD-ME2
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
;
Arabidopsis thaliana
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
2 * 58000, beta-subunit, isozyme NAD-ME2, SDS-PAGE
Arabidopsis thaliana
65000
-
2 * 65000, alpha-subunit, isozyme NAD-ME1, SDS-PAGE
Arabidopsis thaliana
117500
-
gel filtration, isozyme NAD-ME2
Arabidopsis thaliana
120000
-
gel filtration, isozyme NAD-ME1
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-malate + NAD+
Arabidopsis thaliana
the enzyme plays a central role in the metabolite flux through the tricarboxylic acid cycle, overview
pyruvate + CO2 + NADH
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q8L7K9
isozyme NAD-ME2; isozyme NAD-ME2 encoded by gene AtNAD-ME2
-
Arabidopsis thaliana
Q9SIU0
isozyme NAD-ME1; isozyme NAD-ME1 encoded by gene AtNAD-ME1
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
leaf crude extracts contain about 20% higher NAD-ME specific activities at the end of the night period than at the end of the day period, isozyme NAD-ME1 is more abundant during the night period; leaf crude extracts contain about 20% higher NAD-ME specific activities at the end of the night period than at the end of the day period, isozyme NAD-ME2 is more abundant during the night period
Arabidopsis thaliana
-
additional information
tissue specific expression of the isozyme, overview; tissue specific expression of the isozyme, overview
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-malate + NAD+
-
689604
Arabidopsis thaliana
pyruvate + CO2 + NADH
-
-
-
r
(S)-malate + NAD+
the enzyme plays a central role in the metabolite flux through the tricarboxylic acid cycle, overview
689604
Arabidopsis thaliana
pyruvate + CO2 + NADH
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 58000, beta-subunit, isozyme NAD-ME2, SDS-PAGE; 2 * 65000, alpha-subunit, isozyme NAD-ME1, SDS-PAGE
Arabidopsis thaliana
More
the isozyme NAD-ME1 is grouped into the clade that includes enzymes with alpha-subunits with molecular masses of approximately 65 kDa in the plant NAD-ME phylogenetic tree, isozymes NAD-ME1 and NAD-ME2 form both homo- and heterooligomers in vitro and in vivo, overview; the isozyme NAD-ME2 is grouped into the clades with enzymes possessing beta-subunits with molecular masses of approximately 58 kD in the plant NAD-ME phylogenetic tree, isozymes NAD-ME1 and NAD-ME2 form both homo- and heterooligomers in vitro and in vivo, overview
Arabidopsis thaliana
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
31.1
-
(S)-malate
pH 7.4, 30°C, isozyme NAD-ME1
Arabidopsis thaliana
44.1
-
(S)-malate
pH 7.4, 30°C, isozyme NAD-ME2
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
-
Arabidopsis thaliana
6.6
-
-
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
;
Arabidopsis thaliana
NADH
;
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
gene AtNAD-ME1, DNA and amino acid sequence determination and analysis, phylogenetic tree
Arabidopsis thaliana
gene AtNAD-ME2, DNA and amino acid sequence determination and analysis, phylogenetic tree
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Arabidopsis thaliana
NADH
-
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.5
-
NAD+
pH 7.4, 30°C, isozyme NAD-ME1
Arabidopsis thaliana
0.5
-
NAD+
pH 7.4, 30°C, isozyme NAD-ME2
Arabidopsis thaliana
3
-
(S)-malate
pH 7.4, 30°C, isozyme NAD-ME1
Arabidopsis thaliana
3
-
(S)-malate
pH 7.4, 30°C, isozyme NAD-ME2
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Arabidopsis thaliana
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
2 * 58000, beta-subunit, isozyme NAD-ME2, SDS-PAGE
Arabidopsis thaliana
65000
-
2 * 65000, alpha-subunit, isozyme NAD-ME1, SDS-PAGE
Arabidopsis thaliana
117500
-
gel filtration, isozyme NAD-ME2
Arabidopsis thaliana
120000
-
gel filtration, isozyme NAD-ME1
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-malate + NAD+
Arabidopsis thaliana
the enzyme plays a central role in the metabolite flux through the tricarboxylic acid cycle, overview
pyruvate + CO2 + NADH
-
-
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
leaf crude extracts contain about 20% higher NAD-ME specific activities at the end of the night period than at the end of the day period, isozyme NAD-ME1 is more abundant during the night period
Arabidopsis thaliana
-
leaf
leaf crude extracts contain about 20% higher NAD-ME specific activities at the end of the night period than at the end of the day period, isozyme NAD-ME2 is more abundant during the night period
Arabidopsis thaliana
-
additional information
tissue specific expression of the isozyme, overview
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-malate + NAD+
-
689604
Arabidopsis thaliana
pyruvate + CO2 + NADH
-
-
-
r
(S)-malate + NAD+
the enzyme plays a central role in the metabolite flux through the tricarboxylic acid cycle, overview
689604
Arabidopsis thaliana
pyruvate + CO2 + NADH
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 65000, alpha-subunit, isozyme NAD-ME1, SDS-PAGE
Arabidopsis thaliana
dimer
2 * 58000, beta-subunit, isozyme NAD-ME2, SDS-PAGE
Arabidopsis thaliana
More
the isozyme NAD-ME1 is grouped into the clade that includes enzymes with alpha-subunits with molecular masses of approximately 65 kDa in the plant NAD-ME phylogenetic tree, isozymes NAD-ME1 and NAD-ME2 form both homo- and heterooligomers in vitro and in vivo, overview
Arabidopsis thaliana
More
the isozyme NAD-ME2 is grouped into the clades with enzymes possessing beta-subunits with molecular masses of approximately 58 kD in the plant NAD-ME phylogenetic tree, isozymes NAD-ME1 and NAD-ME2 form both homo- and heterooligomers in vitro and in vivo, overview
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
31.1
-
(S)-malate
pH 7.4, 30°C, isozyme NAD-ME1
Arabidopsis thaliana
44.1
-
(S)-malate
pH 7.4, 30°C, isozyme NAD-ME2
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
-
Arabidopsis thaliana
6.6
-
-
Arabidopsis thaliana
Other publictions for EC 1.1.1.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738272
Niedzwiecka
NAD-preferring malic enzyme: l ...
Clupea harengus
Fish Physiol. Biochem.
43
351-360
2017
-
-
-
-
-
-
1
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
1
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
722082
Niedzwiecka
Purification and properties of ...
Clupea harengus
Comp. Biochem. Physiol. B
164
216-220
2013
-
-
-
-
-
-
-
5
-
1
1
1
-
6
-
-
1
-
-
1
2
-
2
1
1
-
-
-
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
5
-
1
1
1
-
-
-
1
-
1
2
-
2
1
1
-
-
-
2
2
-
-
-
-
-
-
-
-
721353
Zhang
NAD(P)+-malic enzyme mutants o ...
Azorhizobium caulinodans, Sinorhizobium sp., Sinorhizobium sp. NGR234
Appl. Environ. Microbiol.
78
2803-2812
2012
4
-
1
-
1
-
2
6
-
-
-
5
-
13
-
-
1
-
-
-
1
-
5
-
1
-
-
-
1
-
-
4
-
-
-
4
-
1
4
-
1
-
-
2
-
6
-
-
-
5
-
-
-
1
-
-
1
-
5
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
721357
Rodriguez
Impact of malic enzymes on ant ...
Streptomyces coelicolor, Streptomyces coelicolor M145
Appl. Environ. Microbiol.
78
4571-4579
2012
-
-
1
-
-
-
-
2
-
1
-
2
-
4
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
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-
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-
1
1
-
-
-
-
-
-
2
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1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
721778
Fu
-
The effects of abiotic stresse ...
Triticum aestivum, Triticum aestivum Jinmai 47
Biol. Plant.
55
196-200
2011
-
-
1
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
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1
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1
1
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-
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-
-
1
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
723540
Hsieh
Determinants of nucleotide-bin ...
Ascaris suum
PLoS ONE
6
e25312
2011
-
-
-
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710948
Landete
Requirement of the Lactobacill ...
Lactobacillus casei, Lactobacillus casei BL23 and ATCC 334
Appl. Environ. Microbiol.
76
84-95
2010
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
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1
-
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1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
3
3
2
-
-
711147
Tronconi
NAD-malic enzymes of Arabidops ...
Arabidopsis thaliana
Biochem. J.
430
295-303
2010
-
-
1
-
1
-
7
3
1
1
-
2
-
4
-
-
1
1
-
-
-
-
3
1
-
-
-
2
1
-
-
1
10
-
-
-
-
2
2
-
2
-
-
11
10
4
2
2
-
2
-
-
-
2
-
-
-
-
3
2
-
-
-
2
2
-
-
-
-
2
4
-
-
-
711524
Sato
Isolation and properties of ma ...
Rhodopseudomonas palustris, Rhodopseudomonas palustris No. 7
Biosci. Biotechnol. Biochem.
74
75-81
2010
-
-
1
-
-
-
7
5
-
6
2
4
-
2
-
-
1
-
-
-
1
1
6
1
1
-
2
4
1
-
1
3
2
1
-
-
-
1
3
-
-
-
-
7
2
5
-
6
2
4
-
-
-
1
-
-
1
1
6
1
1
-
2
4
1
-
1
1
-
2
2
-
4
4
712417
Tronconi
Three different and tissue-spe ...
Arabidopsis thaliana
J. Biol. Chem.
285
11870-11879
2010
8
-
1
-
1
-
-
5
2
1
1
3
-
5
-
-
1
-
-
4
-
-
9
1
-
-
-
6
3
-
-
1
-
-
-
21
-
3
3
-
2
-
-
-
-
9
3
3
1
3
-
-
-
3
-
12
-
-
9
3
-
-
-
6
3
-
-
-
-
1
3
-
6
6
713373
Ren
Induction of erythroid differe ...
Homo sapiens
PLoS ONE
5
e12520
2010
-
1
-
-
1
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1
-
-
1
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3
-
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2
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1
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1
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1
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1
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1
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1
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1
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2
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1
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1
1
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-
712942
Pongratz
Investigating the roles of mit ...
Rattus norvegicus
Methods Enzymol.
457
425-450
2009
-
-
1
-
1
-
-
1
1
1
-
1
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1
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1
-
-
2
-
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1
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1
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1
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1
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1
1
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1
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1
1
1
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1
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1
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2
-
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1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
713212
Rzepka
-
Effect of abiotic stress facto ...
Mnium undulatum, Pilosella officinarum, Polytrichum commune, Polytrichum piliferum
Photosynthetica
47
141-145
2009
-
-
-
-
-
-
4
-
8
4
-
8
-
4
-
-
-
-
-
4
4
-
8
-
4
-
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-
4
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8
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8
-
-
-
-
4
-
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8
4
-
8
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-
4
4
-
8
-
4
-
-
-
4
-
-
-
-
-
-
-
-
-
685215
Aktas
Proper positioning of the nico ...
Ascaris suum
Biochemistry
47
2539-2546
2008
-
-
-
-
7
-
-
15
1
1
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
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2
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-
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-
-
2
-
7
-
-
-
-
15
1
1
-
1
-
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-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
685678
Ohno
Reverse reaction of malic enzy ...
Brevundimonas diminuta, Brevundimonas diminuta IFO 13182
Biosci. Biotechnol. Biochem.
72
1278-1282
2008
-
1
-
-
1
-
-
-
-
1
-
2
-
2
-
-
1
-
-
-
1
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
1
-
-
-
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-
-
1
-
2
-
-
-
1
-
-
1
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
689604
Tronconi
Arabidopsis NAD-malic enzyme f ...
Arabidopsis thaliana
Plant Physiol.
146
1540-1552
2008
-
-
1
-
-
-
-
2
1
-
4
2
-
2
-
-
-
-
-
2
-
-
4
2
-
-
-
2
2
-
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2
-
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2
4
-
-
-
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-
-
4
2
-
4
2
-
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-
-
4
-
-
4
4
-
-
-
2
2
-
-
-
-
-
-
-
-
-
685123
Karsten
Multiple roles of arginine 181 ...
Ascaris suum
Biochemistry
46
14578-14588
2007
-
-
-
-
2
-
1
8
-
2
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
3
2
-
-
-
-
-
3
-
2
-
-
1
2
8
-
2
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654720
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Ascaris suum NAD-malic enzyme ...
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Alternative substrates for mal ...
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Crystal structure of the malic ...
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286706
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Characterization of two member ...
Sinorhizobium meliloti
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286720
Chen
Purification and characterizat ...
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286719
Farias de Aragao
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Purification and partial chara ...
Vigna unguiculata
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286717
Hrdy
Purification and partial chara ...
Tritrichomonas suis
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286718
Suye
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Purification and properties of ...
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286701
Willeford
pH Effects on the activity and ...
Solanum tuberosum
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84
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286702
Willeford
Evidence for a multiple subuni ...
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Willeford
Regulation of the NAD malic en ...
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286700
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NAD-malic enzyme from plants ...
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Activation of NAD-linked malic ...
Brassica oleracea, Solanum tuberosum
Arch. Biochem. Biophys.
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233-242
1984
2
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Canellas
Kinetic properties of NAD mali ...
Brassica oleracea
Arch. Biochem. Biophys.
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1984
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1
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286707
Wedding
Physical and kinetic propertie ...
Crassula argentea
Plant Physiol.
72
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1983
6
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7
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2
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6
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286708
Hatch
Determination of NAD malic enz ...
Amaranthus retroflexus
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Wedding
Slow transients in the activit ...
Crassula argentea
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1981
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-
-
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2
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286710
Grover
Purification of NAD malic enzy ...
Solanum tuberosum
Arch. Biochem. Biophys.
209
396-407
1981
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3
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1
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-
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2
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8
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2
3
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1
-
1
1
-
2
1
-
-
-
1
3
-
-
-
-
-
-
-
-
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286711
Canellas
Substrate and metal ion intera ...
Brassica oleracea
Arch. Biochem. Biophys.
199
259-264
1980
-
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-
-
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1
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1
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2
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-
-
-
1
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1
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1
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2
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-
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286712
Hoek
Nicotinamide-adenine dinucleot ...
Apis mellifera, Glossina austeni, Glossina longipennis, Glossina morsitans, Glossina pallidipes, Heliocarpus sp., Odontotermes sp., Periplaneta americana, Sarcophaga sp., Schistocerca gregaria, Spodoptera exempta
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12
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1
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12
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12
-
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2
2
2
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1
1
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12
-
-
-
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1
1
-
-
-
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-
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286713
Hatch
Properties of leaf NAD malic e ...
Amaranthus edulis, Atriplex spongiosa, Panicum miliaceum
Arch. Biochem. Biophys.
165
188-200
1974
4
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1
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4
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2
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4
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4
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4
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1
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4
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6
1
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1
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3
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1
4
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-
-
-
-
2
-
-
-
-
-
-
-
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286714
Mounib
NAD- and NADP-malic enzymes in ...
Bos taurus, Gadidae, Homo sapiens, salmon
FEBS Lett.
48
79-84
1974
-
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4
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4
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4
-
-
-
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286715
Lin
Malic enzymes of rabbit heart ...
Oryctolagus cuniculus
J. Biol. Chem.
249
3867-3875
1974
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2
8
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1
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1
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2
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2
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286716
Eyzaguirre
Two malic enzymes in Pseudomon ...
Pseudomonas aeruginosa
J. Bacteriol.
116
215-221
1973
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