Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | 30 mM Na+L-malate instead of K+L-malate reduces enzyme activity to 18% in the presence of 3 mM MnCl2, activity is restored with 5 or 50 mM NH4Cl | Sinorhizobium meliloti |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Sinorhizobium meliloti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | 0.005 mM, 18% inhibition, 0.01 mM, 42% inhibition, 0.1 mM, 68% inhibition | Sinorhizobium meliloti | |
Ca2+ | - |
Sinorhizobium meliloti | |
Cu2+ | - |
Sinorhizobium meliloti | |
Zn2+ | 5 mM ZnCl2 | Sinorhizobium meliloti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.142 | - |
NAD+ | - |
Sinorhizobium meliloti | |
8.6 | - |
L-malate | - |
Sinorhizobium meliloti |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | at 0.002 mM MgCl2 enzyme activity is reduced to 25% without additional divalent cations, activity is restored with 0.5 or 5.0 mM CoCl2 | Sinorhizobium meliloti | |
Cs+ | 30 mM Na+L-malate instead of K+L-malate reduces enzyme activity to 18% in the presence of 3 mM MnCl2, activity is restored with 5 or 50 mM CsCl | Sinorhizobium meliloti | |
K+ | 30 mM Na+L-malate instead of K+L-malate reduces enzyme activity to 18% in the presence of 3 mM MnCl2, activity is restored with 50 mM KCl | Sinorhizobium meliloti | |
Mg2+ | at 0.002 mM MgCl2 enzyme activity is reduced to 25% without additional divalent cations, activity is restored with 0.5 or 5.0 mM MgCl2 | Sinorhizobium meliloti | |
Mn2+ | at 0.002 mM MgCl2 enzyme activity is reduced to 25% without additional divalent cations, activity is restored with 0.5 or 5.0 mM MnCl2 | Sinorhizobium meliloti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Sinorhizobium meliloti | - |
CO2 + pyruvate + NADH | - |
? | |
(S)-malate + NADP+ | Sinorhizobium meliloti | 20% activity compared to cofactor NAD+ | CO2 + pyruvate + NADPH | - |
? | |
Oxaloacetate | Sinorhizobium meliloti | - |
CO2 + pyruvate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | - |
now Sinorhizobium meliloti | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.545 | - |
- |
Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Sinorhizobium meliloti | CO2 + pyruvate + NADH | - |
? | |
(S)-malate + NAD+ | - |
Sinorhizobium meliloti | CO2 + pyruvate + NADH | - |
r | |
(S)-malate + NADP+ | 20% activity compared to cofactor NAD+ | Sinorhizobium meliloti | CO2 + pyruvate + NADPH | - |
? | |
Oxaloacetate | - |
Sinorhizobium meliloti | CO2 + pyruvate | - |
ir |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Sinorhizobium meliloti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sinorhizobium meliloti | |
NADP+ | - |
Sinorhizobium meliloti |