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Literature summary for 1.1.1.377 extracted from

  • Kim, S.M.; Paek, K.H.; Lee, S.B.
    Characterization of NADP+-specific L-rhamnose dehydrogenase from the thermoacidophilic Archaeon Thermoplasma acidophilum. (2012), Extremophiles, 16, 447-454.
    View publication on PubMed
Search for more literature for 1.1.1.377

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 NADP+ pH 7.0, 60°C, cosubstrate: L-lyxose Thermoplasma acidophilum
0.1
-
 NADP+ pH 7.0, 60°C, cosubstrate: L-rhamnose Thermoplasma acidophilum
0.13
-
 NADP+ pH 7.0, 60°C, cosubstrate: L-mannose Thermoplasma acidophilum
0.46
-
 L-rhamnose pH 7.0, 60°C Thermoplasma acidophilum
1.37
-
 L-Lyxose pH 7.0, 60°C Thermoplasma acidophilum
6.74
-
 L-Mannose pH 7.0, 60°C Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31237
-
 x * 31237, calculated from sequence4 Thermoplasma acidophilum
34000
-
 x * 34000, SDS-PAGE Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-rhamnose + NADP+ Thermoplasma acidophilum first enzyme of the non-phosphorylative L-rhamnose pathway in archaea L-rhamno-1,4-lactone + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum G3LGQ3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.2
-
 pH 7.0, 60°C, substrates: L-mannose + NADP+ Thermoplasma acidophilum
35.1
-
 pH 7.0, 60°C, substrates: L-lyxose + NADP+ Thermoplasma acidophilum
36.2
-
 pH 7.0, 60°C, substrates: L-rhamnose + NADP+ Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lyxose + NADP+
-
 Thermoplasma acidophilum ?
-
 ?
L-mannose + NADP+
-
 Thermoplasma acidophilum ?
-
 ?
L-rhamnose + NADP+
-
 Thermoplasma acidophilum L-rhamno-1,4-lactone + NADPH + H+
-
 ?
L-rhamnose + NADP+ first enzyme of the non-phosphorylative L-rhamnose pathway in archaea Thermoplasma acidophilum L-rhamno-1,4-lactone + NADPH + H+
-
 ?
additional information less than 1% activity is observed for D-mannose, myo-inositol, D-glyceraldehyde, and D-talose. No catalytic activity is detected for the following substrates: D-glucose, D-galactose, L-galactose, D-arabinose, L-arabinose, Dxylose, L-xylose, D-fucose, L-fucose, D-fructose, D-mannitol, D-xylitol, D-sorbitol, D-ribose, D-deoxyribose, D-glucosamine, N-acetyl-D-glucosamine, and D-altrose Thermoplasma acidophilum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Thermoplasma acidophilum
? x * 31237, calculated from sequence4 Thermoplasma acidophilum

Synonyms

Synonyms Comment Organism
NADP+-specific L-rhamnose dehydrogenase
-
 Thermoplasma acidophilum
RhaD
-
 Thermoplasma acidophilum
Ta0747P locus name Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 activity towards L-rhamnose Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.88
-
 L-Mannose pH 7.0, 60°C Thermoplasma acidophilum
16.5
-
 NADP+ pH 7.0, 60°C, cosubstrate: L-lyxose Thermoplasma acidophilum
17.1
-
 NADP+ pH 7.0, 60°C, cosubstrate: L-rhamnose Thermoplasma acidophilum
18.08
-
 NADP+ pH 7.0, 60°C, cosubstrate: L-mannose Thermoplasma acidophilum
22.36
-
 L-rhamnose pH 7.0, 60°C Thermoplasma acidophilum
23.77
-
 L-Lyxose pH 7.0, 60°C Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 activity towards L-rhamnose Thermoplasma acidophilum

Cofactor

Cofactor Comment Organism Structure
NADP+ no activity with NAD+ Thermoplasma acidophilum

General Information

General Information Comment Organism
physiological function first enzyme of the non-phosphorylative L-rhamnose pathway in archaea Thermoplasma acidophilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2
-
 L-Mannose pH 7.0, 60°C Thermoplasma acidophilum
17.35
-
 L-Lyxose pH 7.0, 60°C Thermoplasma acidophilum
48.6
-
 L-rhamnose pH 7.0, 60°C Thermoplasma acidophilum
139
-
 NADP+ pH 7.0, 60°C, cosubstrate: L-mannose Thermoplasma acidophilum
165
-
 NADP+ pH 7.0, 60°C, cosubstrate: L-lyxose Thermoplasma acidophilum
171
-
 NADP+ pH 7.0, 60°C, cosubstrate: L-rhamnose Thermoplasma acidophilum