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Literature summary for 1.1.1.371 extracted from
- Identification of two scyllo-inositol dehydrogenases in Bacillus subtilis (2010), Microbiology, 156, 1538-1546.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as a C-terminally His6-tagged fusion protein | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
NADPH | pH 7.5, 37°C | Bacillus subtilis |  |
| 0.096 | - |
NADP+ | pH 7.5, 37°C | Bacillus subtilis | |
| 1.68 | - |
2,4,6/3,5-pentahydroxycyclohexanone | pH 7.5, 37°C | Bacillus subtilis | |
| 34.3 | - |
scyllo-Inositol | pH 7.5, 37°C | Bacillus subtilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | O32223 | - |
- |
| Bacillus subtilis 168 | O32223 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:scyllo-inositol-grown cell | - |
Bacillus subtilis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| scyllo-inositol + NADP+ | no activity with NAD+ | Bacillus subtilis | 2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+ | 2,4,6/3,5-pentahydroxycyclohexanone i.e. (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone i.e. scyllo-inosose | r | |
| scyllo-inositol + NADP+ | no activity with NAD+ | Bacillus subtilis 168 | 2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+ | 2,4,6/3,5-pentahydroxycyclohexanone i.e. (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone i.e. scyllo-inosose | r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IolW | - |
Bacillus subtilis |
| yvaA | formerly, gene name | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | no activity with NAD+ | Bacillus subtilis | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Bacillus subtilis | induced during growth on scyllo-inositol | up |
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