KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pH-dependent kinetic mechanism for cMDH, and kinetic modelling for cMDH-catalyzed oxidation of L-malate, detailed overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Homo sapiens | - |
oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | Homo sapiens | - |
(S)-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P40925 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-malate + NAD+ = oxaloacetate + NADH + H+ | an ordered bi-bi mechanism with coenzyme binding first followed by the binding of substrate is able to explain the kinetic data. The proposed mechanism is similar to, but not identical to, the mechanism determined for the mitochondrial isoform, mMDH | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Homo sapiens | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Homo sapiens | (S)-malate + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
cMDH | - |
Homo sapiens |
cytosolic malate dehydrogenase | - |
Homo sapiens |
Mdh1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
NAD+ reduction | Homo sapiens |
9 | - |
NADH oxidation | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NADH | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | malate dehydrogenase utilizes NAD/NADH as coenzyme to reversibly catalyze the oxidation/reduction of the malate/oxaloacetate. The mitochondrial isoenzyme (mMDH) catalyzes the oxidation of malate, and is the last step of the citric acid cycle, while the cytoplasmic isoenzyme (cMDH) primarily reduces oxaloacetate in the cytoplasm | Homo sapiens |
additional information | the active loop on cMDH closing after sequential binding of NADH binding to the enzyme followed by the substrate | Homo sapiens |