Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31600 | - |
- |
Zea mays |
31600 | - |
2 * 31600, SDS-PAGE | Zea mays |
63300 | - |
enzyme form 2, gel fitration | Zea mays |
126600 | - |
enzyme form 1, gel fitration | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Zea mays | - |
oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | Zea mays | - |
(S)-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native tetrameric and dimeric MDH enzyme forms, 46 and 71fold, respectively, from mesophyll of maize leaves to homogeneity by ammonium sulfate fractionation, gel filtration, and anion excange chromatography | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
mesophyll | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
640 | - |
purified native enzyme form 1, malate oxidation, pH 8.0, 25°C | Zea mays |
990 | - |
purified native enzyme form 2, malate oxidation, pH 8.0, 25°C | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Zea mays | oxaloacetate + NADH + H+ | - |
r | |
additional information | the tetrameric MDH enzyme form has a higher affinity for NADH and oxaloacetate, and the dimeric has a higher affinity for NAD+ and malate | Zea mays | ? | - |
? | |
oxaloacetate + NADH + H+ | - |
Zea mays | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31600, SDS-PAGE | Zea mays |
tetramer | 4 * 31600, SDS-PAGE | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Zea mays |
NAD+-dependent malate dehydrogenase | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
both reaction directions, assay at | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | oxaloacetate reduction | Zea mays |
8.5 | 9.5 | malate oxidation | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Zea mays | |
NADH | - |
Zea mays |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the Krebs cycle (catabolism), glyoxylate and Hatch-Slack cycles, and malate metabolism, as well as other anabolic processes | Zea mays |