Crystallization (Comment) | Organism |
---|---|
mutant EX7 (with altered coenzyme specificity from NADH towards NADPH) in complex with NADPH or NADH, 2.0 A resolution | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
NADH | 30°C, pH 7.0, wild-type enzyme | Thermus thermophilus | |
0.003 | - |
oxaloacetate | 30°C, pH 7.0, wild-type enzyme, cofactor NADH | Thermus thermophilus | |
0.0426 | - |
NADPH | 30°C, pH 7.0, wild-type enzyme | Thermus thermophilus | |
0.74 | - |
oxaloacetate | 30°C, pH 7.0, wild-type enzyme, cofactor NADPH | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | P10584 | wild-type enzyme and mutant EX7 (with altered coenzyme specificity from NADH towards NADPH) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADPH + H+ | - |
Thermus thermophilus | (S)-malate + NADP+ | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Thermus thermophilus | |
NADPH | mutant EX7 with altered coenzyme specificity from NADH towards NADPH | Thermus thermophilus |