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Literature summary for 1.1.1.37 extracted from
- Purification and characterization of an NAD-dependent malate dehydrogenase from leaves of the crassulacean acid metabolism plant Aptenia cordifolia (2003), Plant Physiol. Biochem., 41, 97-105.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is sensitive to proteolytic attack | Mesembryanthemum cordifolium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| oxaloacetate | substrate inhibition, pH-dependent | Mesembryanthemum cordifolium |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0104 | - |
NADH | pH 6.9, 30°C | Mesembryanthemum cordifolium | |
| 0.017 | - |
NADH | pH 7.5, 30°C | Mesembryanthemum cordifolium | |
| 0.0391 | - |
oxaloacetate | pH 6.9, 30°C | Mesembryanthemum cordifolium | |
| 0.111 | - |
oxaloacetate | pH 7.5, 30°C | Mesembryanthemum cordifolium | |
| 0.1338 | - |
NAD+ | pH 7.5, 30°C | Mesembryanthemum cordifolium | |
| 0.1397 | - |
NAD+ | pH 6.9, 30°C | Mesembryanthemum cordifolium | |
| 2.068 | - |
L-malate | pH 6.9, 30°C | Mesembryanthemum cordifolium | |
| 2.28 | - |
L-malate | pH 7.5, 30°C | Mesembryanthemum cordifolium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39400 | - |
2 * 39400, SDS-PAGE | Mesembryanthemum cordifolium |
| 83000 | - |
gel filtration | Mesembryanthemum cordifolium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mesembryanthemum cordifolium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mesembryanthemum cordifolium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Mesembryanthemum cordifolium | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1740 | - |
- |
Mesembryanthemum cordifolium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, stable for 4 months | Mesembryanthemum cordifolium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxaloacetate + NADH | - |
Mesembryanthemum cordifolium | L-malate + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 39400, SDS-PAGE | Mesembryanthemum cordifolium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
reduction of oxaloacetate | Mesembryanthemum cordifolium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 75 | 35°C: about 40% of maximal activity, 75°C: about 90% of maximal activity, reduction of oxaloacetate | Mesembryanthemum cordifolium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mesembryanthemum cordifolium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
reduction of oxaloacetate | Mesembryanthemum cordifolium |
| 7.2 | 8.4 | oxidation of malate | Mesembryanthemum cordifolium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | pH 6.0: about 45% of maximal activity, pH 8.0: about 50% of maximal activity, reduction of oxaloacetate | Mesembryanthemum cordifolium |
| 6.8 | 9 | pH 6.8: about 40% of maximal activity, pH 9.0: about 65% of maximal activity, oxidation of malate | Mesembryanthemum cordifolium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Mesembryanthemum cordifolium | |
| NADH | - |
Mesembryanthemum cordifolium | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
oxaloacetate | pH 6.9 | Mesembryanthemum cordifolium | |
| 0.6 | - |
oxaloacetate | pH 7.5 | Mesembryanthemum cordifolium | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mesembryanthemum cordifolium | denaturing isoelectric focusing | - |
6 |
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