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Literature summary for 1.1.1.37 extracted from

  • Muslin, E.H.; Dong, L.; Stevens, F.J.; Donnelly, M.; Schiffer, M.; Anderson, L.E.
    Engineering a domain-locking disulfide into a bacterial malate dehydrogenase produces a redox-sensitive enzyme (1995), Biophys. J., 68, 2218-2223.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.37

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli malate dehydrogenase gene cloned into expression vector pASK40 by PCR Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 oxaloacetate mutant L305C Escherichia coli
0.049
-
 oxaloacetate wild-type Escherichia coli
0.052
-
 NADH mutant N122C-L305C(diamide) Escherichia coli
0.056
-
 NADH mutant N122C-L305C(DTT) Escherichia coli
0.061
-
 NADH wild-type Escherichia coli
0.087
-
 NADH mutant L305C Escherichia coli
0.26
-
 NAD+ wild-type Escherichia coli
0.34
-
 oxaloacetate mutant N122C-L305C(DTT) Escherichia coli
0.38
-
 NAD+ mutant N122C-L305C(DTT) Escherichia coli
0.5
-
 NAD+ mutant N122C-L305C(diamide) Escherichia coli
0.58
-
 oxaloacetate mutant N122C-L305C(diamide) Escherichia coli
2.6
-
 L-malate wild-type Escherichia coli
5.3
-
 L-malate mutant N122C-L305C(DTT) Escherichia coli
6.8
-
 L-malate mutant N122C-L305C(diamide) Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Spinacia oleracea 9507
-
chloroplast
-
 Pisum sativum 9507
-
chloroplast
-
 Zea mays 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malate + NAD+ Sus scrofa
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Spinacia oleracea
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Pisum sativum
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Zea mays
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Synechocystis sp.
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Escherichia coli
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Synechocystis sp. 6803 CF
-
 oxaloacetate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli P61889
-
-
Pisum sativum
-
 pea
-
Spinacia oleracea
-
 spinach
-
Sus scrofa
-
 pig
-
Synechocystis sp.
-
-
-
Synechocystis sp. 6803 CF
-
-
-
Zea mays
-
 maize
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Sus scrofa oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Spinacia oleracea oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Pisum sativum oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Zea mays oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Synechocystis sp. oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Synechocystis sp. 6803 CF oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Sus scrofa oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Spinacia oleracea oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Pisum sativum oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Zea mays oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Synechocystis sp. oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Synechocystis sp. 6803 CF oxaloacetate + NADH + H+
-
 r
oxaloacetate + NADH
-
 Sus scrofa L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Spinacia oleracea L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Pisum sativum L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Zea mays L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Synechocystis sp. L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Escherichia coli L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Synechocystis sp. 6803 CF L-malate + NAD+
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 mutant N122C-L305C Escherichia coli