Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.37 extracted from

  • Iijima, S.; Saiki, T.; Beppu, T.
    Physicochemical and catalytic properties of thermostable malate dehydrogenase from an extreme thermophile Thermus flavus AT-62 (1980), Biochim. Biophys. Acta, 613, 1-9.
    View publication on PubMed
Search for more literature for 1.1.1.37

General Stability

General Stability Organism
remarkably resistant to urea and SDS Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
diethyldicarbonate
-
 Thermus thermophilus
oxaloacetate
-
 Comamonas testosteroni
oxaloacetate
-
 Sus scrofa
oxaloacetate substrate inhibition Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 oxaloacetate 30°C Thermus thermophilus
0.0098
-
 NADH 30°C Thermus thermophilus
0.019
-
 L-malate 30°C Thermus thermophilus
0.02
-
 oxaloacetate 60°C Thermus thermophilus
0.026
-
 NAD+ 30°C Thermus thermophilus
0.03
-
 NADH 60°C Thermus thermophilus
0.095
-
 L-malate 60°C Thermus thermophilus
0.14
-
 NAD+ 60°C Thermus thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Thermus thermophilus 5739
-
mitochondrion
-
 Sus scrofa 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Thermus thermophilus
60000
-
-
 Escherichia coli
60000
-
-
 Sus scrofa
60000
-
 gel filtration Thermus thermophilus
67000
-
-
 Sus scrofa
67000
-
 sedimentation equilibrium centrifugation Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malate + NAD+ Bacillus subtilis
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Thermus thermophilus
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Escherichia coli
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Sus scrofa
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Geobacillus stearothermophilus
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Comamonas testosteroni
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Thermus thermophilus AT-62
-
 oxaloacetate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Comamonas testosteroni
-
-
-
Escherichia coli
-
-
-
Geobacillus stearothermophilus
-
-
-
Sus scrofa
-
 pig
-
Thermus thermophilus
-
 AT-62
-
Thermus thermophilus AT-62
-
 AT-62
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Bacillus subtilis oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Thermus thermophilus oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Sus scrofa oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Geobacillus stearothermophilus oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Comamonas testosteroni oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Thermus thermophilus AT-62 oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Bacillus subtilis oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Thermus thermophilus oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Sus scrofa oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Geobacillus stearothermophilus oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Comamonas testosteroni oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Thermus thermophilus AT-62 oxaloacetate + NADH + H+
-
 r
oxaloacetate + NADH
-
 Bacillus subtilis L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Thermus thermophilus L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Escherichia coli L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Sus scrofa L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Geobacillus stearothermophilus L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Comamonas testosteroni L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Thermus thermophilus AT-62 L-malate + NAD+
-
 r

Subunits

Subunits Comment Organism
dimer
-
 Sus scrofa
dimer
-
 Comamonas testosteroni
dimer 2 * 35000, SDS-PAGE Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 remarkably heat stable without losing activity after incubation for 60 min, 50% activity lost after 30 min at 96°C Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 oxaloacetate reduction, 60°C Thermus thermophilus
9.5
-
 30°C Thermus thermophilus