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Literature summary for 1.1.1.37 extracted from

  • Sprott, G.D.; McKellar, R.C.; Shaw, K.M.; Giroux, J.; Martin, W.G.
    Properties of malate dehydrogenase isolated from Methanospirillum hungatii (1978), Can. J. Microbiol., 25, 192-200.
    View publication on PubMed
Search for more literature for 1.1.1.37

Inhibitors

Inhibitors Comment Organism Structure
2',3'-Cyclic AMP
-
 Methanospirillum hungatei
acetyl-CoA
-
 Methanospirillum hungatei
ADP
-
 Methanospirillum hungatei
alpha-ketoglutarate end-product inhibition Methanospirillum hungatei
ATP
-
 Methanospirillum hungatei
L-malate
-
 Methanospirillum hungatei
L-malate
-
 Sus scrofa
NADH
-
 Methanospirillum hungatei
NADH
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 oxaloacetate
-
 Methanospirillum hungatei
0.081
-
 NADH
-
 Methanospirillum hungatei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61700
-
 gel filtration Methanospirillum hungatei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malate + NAD+ Escherichia coli
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Sus scrofa
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Saccharomyces cerevisiae
-
 oxaloacetate + NADH + H+
-
 r
malate + NAD+ Methanothermobacter thermautotrophicus
-
 oxaloacetate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Methanospirillum hungatei
-
 type strain JF1, strain GP1
-
Methanothermobacter thermautotrophicus
-
-
-
Saccharomyces cerevisiae
-
 yeast
-
Sus scrofa
-
 pig
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanospirillum hungatei

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.24
-
-
 Methanospirillum hungatei

Storage Stability

Storage Stability Organism
4°C, storage in Tris-HCl, 0.01 M, pH 8, 0.2 M NaCl results in a 96% loss of activity within 48 h, loss of activity can be prevented completely for at least 4 weeks by storage in the same buffer, containing 1 M NaCl, under the latter conditions the enzyme is inactive, activity is completely restored upon dilution of the salt to 20 mM Methanospirillum hungatei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Sus scrofa oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Saccharomyces cerevisiae oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Methanothermobacter thermautotrophicus oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Sus scrofa oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Saccharomyces cerevisiae oxaloacetate + NADH + H+
-
 r
malate + NAD+
-
 Methanothermobacter thermautotrophicus oxaloacetate + NADH + H+
-
 r
oxaloacetate + NADH
-
 Escherichia coli L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Sus scrofa L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Saccharomyces cerevisiae L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Methanothermobacter thermautotrophicus L-malate + NAD+
-
 r
oxaloacetate + NADH
-
 Methanospirillum hungatei L-malate + NAD+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Methanospirillum hungatei

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9 no activity remains below pH 5.5 and above pH 11 Methanospirillum hungatei