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Literature summary for 1.1.1.37 extracted from
- Purification and properties of malate dehydrogenase from Pseudomonas testosteroni (1975), J. Bacteriol., 123, 704-716.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-malate | - |
Sus scrofa |  |
| L-malate | - |
Comamonas testosteroni | |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Comamonas testosteroni |
- |
Delftia acidovorans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| oxaloacetate | - |
Comamonas testosteroni | |
| oxaloacetate | - |
Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
NADH | - |
Comamonas testosteroni | |
| 0.057 | - |
oxaloacetate | - |
Comamonas testosteroni | |
| 0.069 | - |
L-malate | 0.03-0.1 mM substrate | Comamonas testosteroni | |
| 0.089 | - |
3-acetylnicotinamide-NAD+ | - |
Comamonas testosteroni | |
| 0.109 | - |
NAD+ | - |
Comamonas testosteroni | |
| 0.23 | - |
thionicotinamide-NAD+ | - |
Comamonas testosteroni | |
| 0.263 | - |
L-malate | 0.2-10 mM substrate | Comamonas testosteroni | |
| 0.264 | - |
deamino-NAD+ | - |
Comamonas testosteroni | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Sus scrofa | 5739 | - |
| mitochondrion | - |
Bos taurus | 5739 | - |
| mitochondrion | - |
Equus sp. | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33500 | - |
4 * 33500, SDS-PAGE | Bacillus subtilis |
| 36000 | - |
2 * 36000, SDS-PAGE | Comamonas testosteroni |
| 43000 | - |
sedimentation equilibrium centrifugation | Delftia acidovorans |
| 60000 | 67000 | sedimentation equilibrium centrifugation | Escherichia coli |
| 70000 | - |
gel filtration, analytical Sephadex | Comamonas testosteroni |
| 74200 | - |
sedimentation equilibrium centrifugation | Comamonas testosteroni |
| 75000 | - |
Svedberg equation | Comamonas testosteroni |
| 117000 | 148000 | sedimentation equilibrium centrifugation | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malate + NAD+ | Bacillus subtilis | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Escherichia coli | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Sus scrofa | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Bos taurus | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Triticum aestivum | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Geobacillus stearothermophilus | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Solanum tuberosum | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Comamonas testosteroni | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Delftia acidovorans | - |
oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | Equus sp. | - |
oxaloacetate + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bos taurus | - |
ox | - |
| Comamonas testosteroni | - |
Stanier strain 78, ATCC 11996 | - |
| Delftia acidovorans | - |
- |
- |
| Equus sp. | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| Solanum tuberosum | - |
potato | - |
| Sus scrofa | - |
pig | - |
| Triticum aestivum | - |
wheat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
- |
Escherichia coli |
- |
Geobacillus stearothermophilus |
- |
Comamonas testosteroni |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| germ | - |
Triticum aestivum | - |
| heart | - |
Sus scrofa | - |
| heart | - |
Bos taurus | - |
| heart | - |
Equus sp. | - |
| tuber | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.762 | - |
- |
Comamonas testosteroni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2S,3S)-tartrate + NAD+ | - |
Comamonas testosteroni | ? + NADH | - |
r | |
| (S)-2-hydroxyglutarate + NAD+ | oxidized at an extremely slow rate, less than 0.001% of the rate of L-malate oxidation | Comamonas testosteroni | 2-oxoglutarate + NADH + H+ | - |
r | |
| (S)-malate + 3-acetylnicotinamide-NAD+ | - |
Comamonas testosteroni | oxaloacetate + 3-acetylnicotinamide-NADH + H+ | - |
r | |
| (S)-malate + deamino-NAD+ | - |
Comamonas testosteroni | oxaloacetate + deamino-NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Bacillus subtilis | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Escherichia coli | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Sus scrofa | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Bos taurus | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Triticum aestivum | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Geobacillus stearothermophilus | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Solanum tuberosum | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Comamonas testosteroni | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Delftia acidovorans | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | - |
Equus sp. | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + thionicotinamide-NAD+ | - |
Comamonas testosteroni | oxaloacetate + thionicotinamide-NADH + H+ | - |
r | |
| alpha-keto-malonate + NADH | - |
Sus scrofa | ? + NAD+ | - |
? | |
| alpha-keto-malonate + NADH | - |
Comamonas testosteroni | ? + NAD+ | - |
ir | |
| hydroxymalonate + NAD+ | - |
Comamonas testosteroni | ? + NADH | - |
? | |
| malate + NAD+ | - |
Bacillus subtilis | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Escherichia coli | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Sus scrofa | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Bos taurus | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Triticum aestivum | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Geobacillus stearothermophilus | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Solanum tuberosum | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Comamonas testosteroni | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Delftia acidovorans | oxaloacetate + NADH + H+ | - |
r | |
| malate + NAD+ | - |
Equus sp. | oxaloacetate + NADH + H+ | - |
r | |
| meso-tartrate + NAD+ | - |
Comamonas testosteroni | ? + NADH | - |
r | |
| additional information | D-malate, (+)-tartrate, succinate and L-aspartate are not oxidized | Comamonas testosteroni | ? | - |
? | |
| oxaloacetate + NADH | - |
Bacillus subtilis | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Escherichia coli | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Sus scrofa | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Bos taurus | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Triticum aestivum | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Geobacillus stearothermophilus | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Solanum tuberosum | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Comamonas testosteroni | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Delftia acidovorans | L-malate + NAD+ | - |
r | |
| oxaloacetate + NADH | - |
Equus sp. | L-malate + NAD+ | - |
r | |
| oxaloglycolate + NAD+ | - |
Comamonas testosteroni | ? + NADH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Sus scrofa |
| dimer | - |
Bos taurus |
| dimer | 2 * 36000, SDS-PAGE | Comamonas testosteroni |
| tetramer | - |
Escherichia coli |
| tetramer | - |
Geobacillus stearothermophilus |
| tetramer | 4 * 33500, SDS-PAGE | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
oxaloacetate reduction | Comamonas testosteroni |
| 10.4 | - |
malate oxidation | Comamonas testosteroni |
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