Cloned (Comment) | Organism |
---|---|
cloned into the yeast expression plasmid pYE352, Saccharomyces cerevisiae fox-2 cells, devoid of Sc MFE-2, transformed with pYE352::ScMFE-2(adelta) and pYE352::ScMFE-2(bdelta). The recombinant (3R)-hydroxyacyl-CoA dehydrogenase [CiMFE-2(h2delta)] transformed into Escherichia coli | Candida tropicalis |
Protein Variants | Comment | Organism |
---|---|---|
A+/B+CtMFE-2(h2delta) | recombinant enzyme | Candida tropicalis |
A-/B+CtMFE-2(hdelta2deltaadelta) | domain A deleted | Candida tropicalis |
A-/B-CtMFE-2(hdelta2deltabdelta) | domain A and B deleted | Candida tropicalis |
G16S | site-directed mutagenesis | Saccharomyces cerevisiae |
G329S | site-directed mutagenesis | Saccharomyces cerevisiae |
additional information | the Candida tropicalis MFE-2 with a deleted hydratase 2 domain (Ct MFE-2(h2delta)) and mutational variants of the A and B domains (Ct MFE-2(h2deltaadelta), Ct MFE-2(h2deltabdelta), and Ct MFE-2(h2deltaadeltabdelta)) are overexpressed and characterized | Candida tropicalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
(3R)-3-hydroxydecanoyl-CoA | A+/B+CtMFE-2(h2delta) | Candida tropicalis | |
0.0054 | - |
(3R)-3-hydroxydecanoyl-CoA | A-/B-CtMFE-2(hdelta2deltabdelta) | Candida tropicalis | |
0.0058 | - |
(3R)-3-hydroxydecanoyl-CoA | A-/B+CtMFE-2(hdelta2deltaadelta) | Candida tropicalis | |
0.024 | - |
(3R)-hydroxyhexadecanoyl-CoA | A+/B+CtMFE-2(h2delta) | Candida tropicalis | |
0.024 | - |
(3R)-hydroxyhexadecanoyl-CoA | A-/B-CtMFE-2(hdelta2deltabdelta) | Candida tropicalis | |
0.025 | - |
(3R)-hydroxyhexadecanoyl-CoA | A-/B+CtMFE-2(hdelta2deltabdelta) | Candida tropicalis | |
0.055 | - |
(3R)-hydroxybutyryl-CoA | A+/B+CtMFE-2(h2delta) | Candida tropicalis | |
0.055 | - |
(3R)-hydroxybutyryl-CoA | A-/B+CtMFE-2(hdelta2deltaadelta) | Candida tropicalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Homo sapiens | 5777 | - |
peroxisome | - |
Saccharomyces cerevisiae | 5777 | - |
peroxisome | - |
Candida tropicalis | 5777 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | - |
alignment of nucleotide-binding site from human MFE-2 with Saccharomyces cerevisiae and Candida tropicalis MFE-2 shown | - |
Homo sapiens | - |
alignment of nucleotide-binding site from human MFE-2 with Saccharomyces cerevisiae and Candida tropicalis MFE-2 shown | - |
Saccharomyces cerevisiae | - |
alignment of nucleotide-binding site from human MFE-2 with Saccharomyces cerevisiae and Candida tropicalis MFE-2 shown | - |
Purification (Comment) | Organism |
---|---|
Candida tropicalis recombinant (3R)-hydroxyacyl-CoA dehydrogenase [CiMFE-2(h2delta)] from Escherichia coli | Candida tropicalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
(3R)-hydroxyacyl-CoA dehydrogenase, Hs MFE-2 | Homo sapiens |
0.5 | - |
(3R)-hydroxyacyl-CoA dehydrogenase, Sc A-/B+ | Saccharomyces cerevisiae |
0.9 | - |
(3R)-hydroxyacyl-CoA dehydrogenase, Sc B-/A+ | Saccharomyces cerevisiae |
1.3 | - |
(3R)-hydroxyacyl-CoA dehydrogenase, Sc MFE-2 | Saccharomyces cerevisiae |
125 | - |
last purification step, 5.9fold purification. Candida tropicalis recombinant (3R)-hydroxyacyl-CoA dehydrogenase [CiMFE-2(h2delta)] from Escherichia coli | Candida tropicalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-3-hydroxydecanoyl-CoA + NADP+ | (3R)-hydroxyacyl-CoA dehydrogenase | Candida tropicalis | 3-oxodecanoyl-CoA + NADPH + H+ | - |
? | |
(3R)-hydroxybutyryl-CoA + NADP+ | - |
Candida tropicalis | 3-oxobutyryl-CoA + NADPH + H+ | - |
? | |
(3R)-hydroxyhexadecanoyl-CoA + NADP+ | - |
Candida tropicalis | 3-oxohexadecanoyl-CoA + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(3R)-hydroxyacyl-CoA dehydrogenase | domain of multifunctional enzyme type 2 (MFE-2), (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 | Homo sapiens |
(3R)-hydroxyacyl-CoA dehydrogenase | domain of multifunctional enzyme type 2 (MFE-2), (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 | Saccharomyces cerevisiae |
(3R)-hydroxyacyl-CoA dehydrogenase | domain of multifunctional enzyme type 2 (MFE-2), (3R)-hydroxyacyl-CoA dehydrogenase/2-enoyl-CoA hydratase 2 | Candida tropicalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | (3R)-hydroxyhexadecanoyl-CoA | A-/B-CtMFE-2(hdelta2delta) | Candida tropicalis | |
12 | - |
(3R)-hydroxyhexadecanoyl-CoA | A-/B+CtMFE-2(hdelta2deltaadelta) | Candida tropicalis | |
17 | - |
(3R)-3-hydroxydecanoyl-CoA | A-/B+CtMFE-2(hdelta2deltaadelta) | Candida tropicalis | |
29 | - |
(3R)-hydroxybutyryl-CoA | A-/B+CtMFE-2(hdelta2deltaadelta) | Candida tropicalis | |
31 | - |
(3R)-hydroxybutyryl-CoA | A+/B+CtMFE-2(h2delta) | Candida tropicalis | |
33 | - |
(3R)-3-hydroxydecanoyl-CoA | A-/B-CtMFE-2(hdelta2delta) | Candida tropicalis | |
41 | - |
(3R)-hydroxyhexadecanoyl-CoA | A+/B+CtMFE-2(h2delta) | Candida tropicalis | |
53 | - |
(3R)-3-hydroxydecanoyl-CoA | A+/B+CtMFE-2(h2delta) | Candida tropicalis |