BRENDA - Enzyme Database show
show all sequences of 1.1.1.36

Purification and characterization of NADPH-dependent acetoacetyl-CoA reductase from Methylobacterium extorquens

Belova, L.L.; Sokolov, A.P.; Sidorov, I.A.; Trotsenko, Y.A.; FEMS Microbiol. Lett. 156, 275-279 (1997)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
alpha-ketoglutarate
20% activation
Methylobacterium extorquens
ATP
32% activation
Methylobacterium extorquens
citrate
30% activation
Methylobacterium extorquens
isocitrate
25% activation, stimulates NADP+ regeneration
Methylobacterium extorquens
Inhibitors
Inhibitors
Commentary
Organism
Structure
acetoacetyl-CoA
-
Azotobacter vinelandii
acetoacetyl-CoA
-
Cupriavidus necator
acetoacetyl-CoA
concentration above 40 mM
Methylobacterium extorquens
acetoacetyl-CoA
-
Methylobacterium rhodesianum
acetoacetyl-CoA
-
Zoogloea ramigera
NAD+
17% inhibition
Methylobacterium extorquens
NAD+
-
Methylobacterium rhodesianum
NADP+
40% inhibition
Methylobacterium extorquens
NADP+
-
Methylobacterium rhodesianum
NADPH
-
Azotobacter vinelandii
NADPH
concentrationgreater 100 mM
Methylobacterium extorquens
NADPH
-
Methylobacterium rhodesianum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
acetoacetyl-CoA
-
Cupriavidus necator
0.011
-
acetoacetyl-CoA
100 mM NADPH
Azotobacter vinelandii
0.0116
-
acetoacetyl-CoA
-
Methylobacterium extorquens
0.015
-
acetoacetyl-CoA
-
Methylobacterium rhodesianum
0.018
-
NADPH
-
Methylobacterium rhodesianum
0.019
-
NADPH
-
Cupriavidus necator
0.02
-
NADPH
32 mM acetoacetyl-CoA
Azotobacter vinelandii
0.03
-
D-beta-hydroxybutyryl-CoA
-
Methylobacterium rhodesianum
0.031
-
NADP+
-
Cupriavidus necator
0.033
-
D-hydroxybutyryl-CoA
-
Cupriavidus necator
0.041
-
NADPH
-
Methylobacterium extorquens
0.06
-
NADP+
-
Methylobacterium rhodesianum
0.25
-
NAD+
-
Cupriavidus necator
0.4
-
NADH
-
Cupriavidus necator
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Methylobacterium extorquens
-
-
soluble
-
Zoogloea ramigera
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
4 * 31000, 12% PAGE
Methylobacterium extorquens
77000
-
-
Azotobacter vinelandii
84000
-
-
Cupriavidus necator
92000
-
-
Zoogloea ramigera
140000
-
gel-filtration
Methylobacterium extorquens
141000
-
gradient PAGE
Methylobacterium extorquens
250000
-
-
Methylobacterium rhodesianum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetoacetyl-CoA + NADH
Zoogloea ramigera
-
L-3-hydroxybutyryl-CoA + NAD+
-
Zoogloea ramigera
?
acetoacetyl-CoA + NADH
Rhodospirillum rubrum
-
L-3-hydroxybutyryl-CoA + NAD+
-
Rhodospirillum rubrum
?
acetoacetyl-CoA + NADPH
Zoogloea ramigera
PHB synthesis
D-3-hydroxybutyryl-CoA + NADP+
-
Zoogloea ramigera
r
acetoacetyl-CoA + NADPH
Cupriavidus necator
PHB synthesis
D-3-hydroxybutyryl-CoA + NADP+
-
Cupriavidus necator
-
acetoacetyl-CoA + NADPH
Methylobacterium extorquens
tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway
D-3-hydroxybutyryl-CoA + NADP+
-
Methylobacterium extorquens
r
acetoacetyl-CoA + NADPH
Methylobacterium extorquens 15
tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway
D-3-hydroxybutyryl-CoA + NADP+
-
Methylobacterium extorquens 15
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Azotobacter vinelandii
-
-
-
Cupriavidus necator
-
-
-
Methylobacterium extorquens
-
15
-
Methylobacterium extorquens 15
-
15
-
Methylobacterium rhodesianum
-
MB 126
-
Methylobacterium rhodesianum MB 126
-
MB 126
-
Rhodospirillum rubrum
-
-
-
Zoogloea ramigera
-
-
-
Purification (Commentary)
Commentary
Organism
PEG, DEAE-Toyopearl column
Methylobacterium extorquens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
184
-
-
Methylobacterium extorquens
Storage Stability
Storage Stability
Organism
0°C, 25 mM Tris-HCl, pH 8.0
Methylobacterium extorquens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetoacetyl-CoA + NADH
-
286622
Zoogloea ramigera
L-3-hydroxybutyryl-CoA + NAD+
-
286622
Zoogloea ramigera
?
acetoacetyl-CoA + NADH
-
286622
Cupriavidus necator
L-3-hydroxybutyryl-CoA + NAD+
-
-
-
-
acetoacetyl-CoA + NADH
-
286622
Rhodospirillum rubrum
L-3-hydroxybutyryl-CoA + NAD+
-
286622
Rhodospirillum rubrum
?
acetoacetyl-CoA + NADPH
-
286622
Methylobacterium rhodesianum
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
-
acetoacetyl-CoA + NADPH
-
286622
Methylobacterium rhodesianum MB 126
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
-
acetoacetyl-CoA + NADPH
PHB synthesis
286622
Zoogloea ramigera
D-3-hydroxybutyryl-CoA + NADP+
-
286622
Zoogloea ramigera
r
acetoacetyl-CoA + NADPH
PHB synthesis
286622
Cupriavidus necator
D-3-hydroxybutyryl-CoA + NADP+
-
286622
Cupriavidus necator
-
acetoacetyl-CoA + NADPH
tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway
286622
Methylobacterium extorquens
D-3-hydroxybutyryl-CoA + NADP+
-
286622
Methylobacterium extorquens
r
acetoacetyl-CoA + NADPH
tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway
286622
Methylobacterium extorquens 15
D-3-hydroxybutyryl-CoA + NADP+
-
286622
Methylobacterium extorquens 15
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 31000, 12% PAGE
Methylobacterium extorquens
tetramer
-
Zoogloea ramigera
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Methylobacterium extorquens
30
-
-
Zoogloea ramigera
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Methylobacterium extorquens
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Azotobacter vinelandii
NADPH
absolute specificity
Methylobacterium extorquens
NADPH
absolute specificity
Zoogloea ramigera
NADPH
-
Methylobacterium rhodesianum
NADPH
-
Cupriavidus necator
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
alpha-ketoglutarate
20% activation
Methylobacterium extorquens
ATP
32% activation
Methylobacterium extorquens
citrate
30% activation
Methylobacterium extorquens
isocitrate
25% activation, stimulates NADP+ regeneration
Methylobacterium extorquens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Azotobacter vinelandii
NADPH
absolute specificity
Methylobacterium extorquens
NADPH
absolute specificity
Zoogloea ramigera
NADPH
-
Methylobacterium rhodesianum
NADPH
-
Cupriavidus necator
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetoacetyl-CoA
-
Azotobacter vinelandii
acetoacetyl-CoA
-
Cupriavidus necator
acetoacetyl-CoA
concentration above 40 mM
Methylobacterium extorquens
acetoacetyl-CoA
-
Methylobacterium rhodesianum
acetoacetyl-CoA
-
Zoogloea ramigera
NAD+
17% inhibition
Methylobacterium extorquens
NAD+
-
Methylobacterium rhodesianum
NADP+
40% inhibition
Methylobacterium extorquens
NADP+
-
Methylobacterium rhodesianum
NADPH
-
Azotobacter vinelandii
NADPH
concentrationgreater 100 mM
Methylobacterium extorquens
NADPH
-
Methylobacterium rhodesianum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
acetoacetyl-CoA
-
Cupriavidus necator
0.011
-
acetoacetyl-CoA
100 mM NADPH
Azotobacter vinelandii
0.0116
-
acetoacetyl-CoA
-
Methylobacterium extorquens
0.015
-
acetoacetyl-CoA
-
Methylobacterium rhodesianum
0.018
-
NADPH
-
Methylobacterium rhodesianum
0.019
-
NADPH
-
Cupriavidus necator
0.02
-
NADPH
32 mM acetoacetyl-CoA
Azotobacter vinelandii
0.03
-
D-beta-hydroxybutyryl-CoA
-
Methylobacterium rhodesianum
0.031
-
NADP+
-
Cupriavidus necator
0.033
-
D-hydroxybutyryl-CoA
-
Cupriavidus necator
0.041
-
NADPH
-
Methylobacterium extorquens
0.06
-
NADP+
-
Methylobacterium rhodesianum
0.25
-
NAD+
-
Cupriavidus necator
0.4
-
NADH
-
Cupriavidus necator
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Methylobacterium extorquens
-
-
soluble
-
Zoogloea ramigera
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
4 * 31000, 12% PAGE
Methylobacterium extorquens
77000
-
-
Azotobacter vinelandii
84000
-
-
Cupriavidus necator
92000
-
-
Zoogloea ramigera
140000
-
gel-filtration
Methylobacterium extorquens
141000
-
gradient PAGE
Methylobacterium extorquens
250000
-
-
Methylobacterium rhodesianum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetoacetyl-CoA + NADH
Zoogloea ramigera
-
L-3-hydroxybutyryl-CoA + NAD+
-
Zoogloea ramigera
?
acetoacetyl-CoA + NADH
Rhodospirillum rubrum
-
L-3-hydroxybutyryl-CoA + NAD+
-
Rhodospirillum rubrum
?
acetoacetyl-CoA + NADPH
Zoogloea ramigera
PHB synthesis
D-3-hydroxybutyryl-CoA + NADP+
-
Zoogloea ramigera
r
acetoacetyl-CoA + NADPH
Cupriavidus necator
PHB synthesis
D-3-hydroxybutyryl-CoA + NADP+
-
Cupriavidus necator
-
acetoacetyl-CoA + NADPH
Methylobacterium extorquens
tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway
D-3-hydroxybutyryl-CoA + NADP+
-
Methylobacterium extorquens
r
acetoacetyl-CoA + NADPH
Methylobacterium extorquens 15
tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway
D-3-hydroxybutyryl-CoA + NADP+
-
Methylobacterium extorquens 15
r
Purification (Commentary) (protein specific)
Commentary
Organism
PEG, DEAE-Toyopearl column
Methylobacterium extorquens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
184
-
-
Methylobacterium extorquens
Storage Stability (protein specific)
Storage Stability
Organism
0°C, 25 mM Tris-HCl, pH 8.0
Methylobacterium extorquens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetoacetyl-CoA + NADH
-
286622
Zoogloea ramigera
L-3-hydroxybutyryl-CoA + NAD+
-
286622
Zoogloea ramigera
?
acetoacetyl-CoA + NADH
-
286622
Cupriavidus necator
L-3-hydroxybutyryl-CoA + NAD+
-
-
-
-
acetoacetyl-CoA + NADH
-
286622
Rhodospirillum rubrum
L-3-hydroxybutyryl-CoA + NAD+
-
286622
Rhodospirillum rubrum
?
acetoacetyl-CoA + NADPH
-
286622
Methylobacterium rhodesianum
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
-
acetoacetyl-CoA + NADPH
-
286622
Methylobacterium rhodesianum MB 126
D-beta-hydroxybutyryl-CoA + NADP+
-
-
-
-
acetoacetyl-CoA + NADPH
PHB synthesis
286622
Zoogloea ramigera
D-3-hydroxybutyryl-CoA + NADP+
-
286622
Zoogloea ramigera
r
acetoacetyl-CoA + NADPH
PHB synthesis
286622
Cupriavidus necator
D-3-hydroxybutyryl-CoA + NADP+
-
286622
Cupriavidus necator
-
acetoacetyl-CoA + NADPH
tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway
286622
Methylobacterium extorquens
D-3-hydroxybutyryl-CoA + NADP+
-
286622
Methylobacterium extorquens
r
acetoacetyl-CoA + NADPH
tricarboxylic acid cycle, poly-3-hydroxybutyrate synthesis, aerobic serine pathway
286622
Methylobacterium extorquens 15
D-3-hydroxybutyryl-CoA + NADP+
-
286622
Methylobacterium extorquens 15
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 31000, 12% PAGE
Methylobacterium extorquens
tetramer
-
Zoogloea ramigera
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Methylobacterium extorquens
30
-
-
Zoogloea ramigera
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Methylobacterium extorquens
Other publictions for EC 1.1.1.36
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
700827
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1
1
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1
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1
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1
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1
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2
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1
2
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1
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1
-
1
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1
-
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1
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-
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-
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687159
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Cupriavidus necator
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-
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1
-
1
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-
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2
-
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-
1
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689821
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Cupriavidus necator
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1
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-
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-
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-
-
1
-
1
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-
-
-
-
-
-
-
1
-
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-
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-
-
-
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2
-
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1
2
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-
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1
-
-
-
-
-
-
-
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1
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
1
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667285
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Effect of expressing polyhydro ...
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4
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1
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1
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1
-
1
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-
-
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-
-
-
-
-
-
669878
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Crystal structure of yeast per ...
Candida tropicalis
J. Mol. Biol.
358
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-
-
1
1
-
-
-
-
1
-
-
-
-
5
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
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Aeromonas hydrophila, Aeromonas hydrophila 4AK4, Pseudomonas putida
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Enzyme-catalyzed poly(3-hydrox ...
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2003
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657106
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Binary structure of the two-do ...
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2
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Qin
Human peroxisomal multifunctio ...
Homo sapiens
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14
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11
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Poly(beta-hydroxybutyrate) pro ...
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Yeast peroxisomal multifunctio ...
Candida tropicalis, Homo sapiens, Saccharomyces cerevisiae
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6
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1
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4
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Qin
Peroxisomal multifunctional en ...
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2
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286622
Belova
-
Purification and characterizat ...
Azotobacter vinelandii, Cupriavidus necator, Methylobacterium extorquens, Methylobacterium extorquens 15, Methylobacterium rhodesianum, Methylobacterium rhodesianum MB 126, Rhodospirillum rubrum, Zoogloea ramigera
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1997
4
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14
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2
2
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1
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12
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2
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7
6
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1
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1
1
9
2
2
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1
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Gerngross
Enzyme-catalyzed synthesis of ...
Cupriavidus necator
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1995
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2
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286623
Mothes
-
Methylobacterium rhodesianum M ...
Methylobacterium rhodesianum, Methylobacterium rhodesianum MB 126, Rhodococcus ruber, Rhodospirillum rubrum, Zoogloea ramigera
Arch. Microbiol.
161
277-280
1994
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5
6
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8
1
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1
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286621
Amos
-
Formation of D-3-hydroxybutyry ...
Azotobacter beijerinckii, Cupriavidus necator, Syntrophomonas wolfei, Zoogloea ramigera
Arch. Microbiol.
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2
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11
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3
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7
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1
1
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687487
Hiltunen
Peroxisomal multifunctional be ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae fox2
J. Biol. Chem.
267
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1992
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6
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2
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-
-
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286620
Ploux
The NADPH-linked acetoacetyl-C ...
Zoogloea ramigera
Eur. J. Biochem.
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177-182
1988
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1
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2
7
1
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3
1
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4
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1
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1
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1
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6
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1
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1
1
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2
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7
1
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3
1
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1
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1
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6
1
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6
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-
-
-
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286619
Fukui
Purification and characterizat ...
Pigeon, Zoogloea ramigera
Biochim. Biophys. Acta
917
365-371
1987
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1
1
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2
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1
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1
2
7
1
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4
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4
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1
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1
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2
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1
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1
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2
1
2
7
1
1
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286618
Cook
Isolation of rat liver microso ...
Rattus norvegicus
Arch. Biochem. Biophys.
246
206-216
1986
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1
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1
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2
1
2
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1
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3
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2
1
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1
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1
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1
2
1
2
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1
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286617
Prasad
Rat hepatic microsomal acetoac ...
Rattus norvegicus
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259
7460-7467
1984
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2
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2
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1
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1
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1
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286615
Dodds
Acetoacetyl-CoA reductase acti ...
Anser sp., Bos taurus, Gallus gallus, Pigeon, Rattus norvegicus, Saccharomyces cerevisiae
J. Biol. Chem.
256
6282-6290
1981
4
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12
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54
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4
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7
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9
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286614
Saito
An NADP-linked acetoacetyl CoA ...
Azotobacter beijerinckii, Zoogloea ramigera
Arch. Microbiol.
114
211-217
1977
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2
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6
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1
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6
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1
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1
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Packter
-
Characterization of acetoacety ...
Streptomyces coelicolor
Biochem. Soc. Trans.
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1973
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2
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2
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3
1
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Wakil
Studies on the mechanism of fa ...
Pigeon
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1962
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1
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