BRENDA - Enzyme Database show
show all sequences of 1.1.1.36

The NADPH-linked acetoacetyl-CoA reductase from Zoogloea ramigera. Characterization and mechanistic studies of the cloned enzyme over-produced in Escherichia coli

Ploux, O.; Masamune, S.; Walsh, C.T.; Eur. J. Biochem. 174, 177-182 (1988)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli JM155
Zoogloea ramigera
Inhibitors
Inhibitors
Commentary
Organism
Structure
acetoacetyl-CoA
above 0.01 mM
Zoogloea ramigera
propionylacetyl-CoA
-
Zoogloea ramigera
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
acetoacetyl-CoA
-
Zoogloea ramigera
0.002
-
propionylacetyl-CoA
-
Zoogloea ramigera
0.01
-
butyrylacetyl-CoA
-
Zoogloea ramigera
0.02
0.021
NADPH
cosubstrate acetoacetyl-CoA
Zoogloea ramigera
0.026
-
D-3-hydroxybutyryl-CoA
-
Zoogloea ramigera
0.74
-
2-methylacetoacetyl-CoA
-
Zoogloea ramigera
0.99
-
Acetoacetyl-S-(D-pantetheine)11-pivalate
-
Zoogloea ramigera
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Zoogloea ramigera
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
4 * 24000 SDS-PAGE
Zoogloea ramigera
25200
-
4 * 25200 gene sequence
Zoogloea ramigera
100000
-
gel filtration
Zoogloea ramigera
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetoacetyl-CoA + NADPH
Zoogloea ramigera
biosynthesis of poly(D-3-hydroxybutyrate)
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zoogloea ramigera
-
gene cloned in Escherichia coli
-
Purification (Commentary)
Commentary
Organism
DEAE-Sepharose chromatography, Blue gel column
Zoogloea ramigera
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
608
-
-
Zoogloea ramigera
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methylacetoacetyl-CoA + NADPH
-
286620
Zoogloea ramigera
2-methyl-3-hydroxybutyryl-CoA + NADP+
-
286620
Zoogloea ramigera
?
acetoacetyl-CoA + NADPH
biosynthesis of poly(D-3-hydroxybutyrate)
286620
Zoogloea ramigera
?
-
-
-
-
acetoacetyl-CoA + NADPH + H+
-
286620
Zoogloea ramigera
(3R)-3-hydroxybutyryl-CoA + NADP+
-
286620
Zoogloea ramigera
r
acetoacetyl-S-(D-pantetheine)11-pivalate + NADPH
-
286620
Zoogloea ramigera
3-hydroxybutyryl-S-(D-pantetheine)11-pivalate + NADP+
-
286620
Zoogloea ramigera
?
butyrylacetyl-CoA + NADPH
-
286620
Zoogloea ramigera
3-hydroxyhexanoyl-CoA + NADP+
-
286620
Zoogloea ramigera
?
propionylacetyl-CoA + NADPH
-
286620
Zoogloea ramigera
3-hydroxypentanoyl-CoA + NADP+
-
286620
Zoogloea ramigera
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 24000 SDS-PAGE; 4 * 25200 gene sequence
Zoogloea ramigera
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9
-
butyrylacetyl-CoA
-
Zoogloea ramigera
27
-
2-methylacetoacetyl-CoA
-
Zoogloea ramigera
90
-
D-3-hydroxybutyryl-CoA
-
Zoogloea ramigera
124
-
propionylacetyl-CoA
-
Zoogloea ramigera
267
-
Acetoacetyl-S-(D-pantetheine)11-pivalate
-
Zoogloea ramigera
300
-
acetoacetyl-CoA
-
Zoogloea ramigera
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
absolute specificity; B-specific, i.e. transfer of pro-S-hydrogen from NADPH to acetoacetyl-CoA; specific for
Zoogloea ramigera
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli JM155
Zoogloea ramigera
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
absolute specificity; B-specific, i.e. transfer of pro-S-hydrogen from NADPH to acetoacetyl-CoA; specific for
Zoogloea ramigera
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetoacetyl-CoA
above 0.01 mM
Zoogloea ramigera
propionylacetyl-CoA
-
Zoogloea ramigera
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
acetoacetyl-CoA
-
Zoogloea ramigera
0.002
-
propionylacetyl-CoA
-
Zoogloea ramigera
0.01
-
butyrylacetyl-CoA
-
Zoogloea ramigera
0.02
0.021
NADPH
cosubstrate acetoacetyl-CoA
Zoogloea ramigera
0.026
-
D-3-hydroxybutyryl-CoA
-
Zoogloea ramigera
0.74
-
2-methylacetoacetyl-CoA
-
Zoogloea ramigera
0.99
-
Acetoacetyl-S-(D-pantetheine)11-pivalate
-
Zoogloea ramigera
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Zoogloea ramigera
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
4 * 24000 SDS-PAGE
Zoogloea ramigera
25200
-
4 * 25200 gene sequence
Zoogloea ramigera
100000
-
gel filtration
Zoogloea ramigera
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetoacetyl-CoA + NADPH
Zoogloea ramigera
biosynthesis of poly(D-3-hydroxybutyrate)
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-Sepharose chromatography, Blue gel column
Zoogloea ramigera
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
608
-
-
Zoogloea ramigera
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-methylacetoacetyl-CoA + NADPH
-
286620
Zoogloea ramigera
2-methyl-3-hydroxybutyryl-CoA + NADP+
-
286620
Zoogloea ramigera
?
acetoacetyl-CoA + NADPH
biosynthesis of poly(D-3-hydroxybutyrate)
286620
Zoogloea ramigera
?
-
-
-
-
acetoacetyl-CoA + NADPH + H+
-
286620
Zoogloea ramigera
(3R)-3-hydroxybutyryl-CoA + NADP+
-
286620
Zoogloea ramigera
r
acetoacetyl-S-(D-pantetheine)11-pivalate + NADPH
-
286620
Zoogloea ramigera
3-hydroxybutyryl-S-(D-pantetheine)11-pivalate + NADP+
-
286620
Zoogloea ramigera
?
butyrylacetyl-CoA + NADPH
-
286620
Zoogloea ramigera
3-hydroxyhexanoyl-CoA + NADP+
-
286620
Zoogloea ramigera
?
propionylacetyl-CoA + NADPH
-
286620
Zoogloea ramigera
3-hydroxypentanoyl-CoA + NADP+
-
286620
Zoogloea ramigera
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 24000 SDS-PAGE; 4 * 25200 gene sequence
Zoogloea ramigera
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
9
-
butyrylacetyl-CoA
-
Zoogloea ramigera
27
-
2-methylacetoacetyl-CoA
-
Zoogloea ramigera
90
-
D-3-hydroxybutyryl-CoA
-
Zoogloea ramigera
124
-
propionylacetyl-CoA
-
Zoogloea ramigera
267
-
Acetoacetyl-S-(D-pantetheine)11-pivalate
-
Zoogloea ramigera
300
-
acetoacetyl-CoA
-
Zoogloea ramigera
Other publictions for EC 1.1.1.36
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
700827
Beaudoin
Functional characterization of ...
Arabidopsis thaliana
Plant Physiol.
150
1174-1191
2009
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1
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1
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1
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1
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1
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6
1
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1
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6
1
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1
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2
2
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-
701808
Han
Identification of the polyhydr ...
Haloarcula hispanica, Haloarcula hispanica DSM 4426
Appl. Environ. Microbiol.
75
6168-6175
2009
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1
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1
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7
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4
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684617
Liu
Microbial production of R-3-hy ...
Cupriavidus necator, no activity in Escherichia coli, no activity in Escherichia coli DH5-alpha
Appl. Microbiol. Biotechnol.
76
811-818
2007
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1
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1
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4
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1
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1
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1
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1
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1
-
-
-
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-
687159
Abd-El-haleem
-
Biosynthesis of biodegradable ...
Cupriavidus necator
Int. J. Environ. Sci. Technol.
4
513-520
2007
-
-
1
-
-
-
-
-
-
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1
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1
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1
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1
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2
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1
2
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1
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1
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1
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689821
Zhao
-
Production and characterizatio ...
Cupriavidus necator
Process Biochem.
42
1342-1347
2007
-
-
1
-
-
-
-
-
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-
1
-
1
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1
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2
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1
2
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1
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-
1
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667282
Qiu
Metabolic engineering of Aerom ...
Aeromonas hydrophila, Cupriavidus necator
Appl. Microbiol. Biotechnol.
69
537-542
2006
-
-
-
-
-
-
-
-
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1
-
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8
-
-
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2
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1
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2
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-
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-
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-
667285
Zhang
Effect of expressing polyhydro ...
Streptococcus equi
Appl. Microbiol. Biotechnol.
71
222-227
2006
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1
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-
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4
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1
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1
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1
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1
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1
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-
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-
669878
Ylianttila
Crystal structure of yeast per ...
Candida tropicalis
J. Mol. Biol.
358
1286-1295
2006
-
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1
1
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1
-
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5
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1
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3
1
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1
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1
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1
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1
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3
1
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668057
Qiu
Production of poly(3-hydroxybu ...
Aeromonas hydrophila, Aeromonas hydrophila 4AK4, Pseudomonas putida
Biotechnol. Lett.
27
1381-1386
2005
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9
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3
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3
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654460
Ylianttila
Site-directed mutagenesis to e ...
Candida tropicalis
Biochem. Biophys. Res. Commun.
324
25-30
2004
-
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1
1
1
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2
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1
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1
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1
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1
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1
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1
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656318
Satoh
Enzyme-catalyzed poly(3-hydrox ...
Cupriavidus necator
J. Biosci. Bioeng.
95
335-341
2003
-
1
1
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1
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2
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1
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1
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1
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1
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1
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1
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1
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1
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1
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657106
Zhong
-
The pea (Pisum sativum L.) rbc ...
Cupriavidus necator
Plant Sci.
165
455-462
2003
-
1
1
-
1
-
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-
-
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1
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1
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2
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1
1
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1
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1
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2
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657366
Haapalainen
Binary structure of the two-do ...
Rattus norvegicus
Structure
11
87-97
2003
-
-
1
1
-
-
-
-
2
-
-
3
-
2
-
-
1
1
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-
3
2
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1
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1
1
1
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2
-
-
3
-
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-
1
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-
-
3
2
-
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-
-
-
-
-
-
-
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-
652075
Qin
Human peroxisomal multifunctio ...
Homo sapiens
J. Biol. Chem.
275
4965-4972
2000
-
-
1
-
14
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
14
-
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1
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-
11
-
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-
-
-
-
-
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-
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-
286625
Houmiel
Poly(beta-hydroxybutyrate) pro ...
Cupriavidus necator
Planta
209
547-550
1999
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
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-
-
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1
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1
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1
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-
687492
Qin
Yeast peroxisomal multifunctio ...
Candida tropicalis, Homo sapiens, Saccharomyces cerevisiae
J. Biol. Chem.
274
28619-28625
1999
-
-
1
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4
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8
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33732
Qin
Peroxisomal multifunctional en ...
Rattus norvegicus
Biochem. J.
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21-28
1997
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2
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2
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286622
Belova
-
Purification and characterizat ...
Azotobacter vinelandii, Cupriavidus necator, Methylobacterium extorquens, Methylobacterium extorquens 15, Methylobacterium rhodesianum, Methylobacterium rhodesianum MB 126, Rhodospirillum rubrum, Zoogloea ramigera
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4
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12
14
2
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7
6
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2
2
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1
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12
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14
2
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7
6
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1
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1
1
9
2
2
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1
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286624
Gerngross
Enzyme-catalyzed synthesis of ...
Cupriavidus necator
Proc. Natl. Acad. Sci. USA
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6279-6283
1995
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1
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2
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286623
Mothes
-
Methylobacterium rhodesianum M ...
Methylobacterium rhodesianum, Methylobacterium rhodesianum MB 126, Rhodococcus ruber, Rhodospirillum rubrum, Zoogloea ramigera
Arch. Microbiol.
161
277-280
1994
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5
6
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3
4
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6
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1
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1
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1
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6
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4
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1
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8
1
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1
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286621
Amos
-
Formation of D-3-hydroxybutyry ...
Azotobacter beijerinckii, Cupriavidus necator, Syntrophomonas wolfei, Zoogloea ramigera
Arch. Microbiol.
159
16-20
1993
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2
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5
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4
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1
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3
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7
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1
1
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11
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11
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2
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5
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1
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3
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7
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1
1
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687487
Hiltunen
Peroxisomal multifunctional be ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae fox2
J. Biol. Chem.
267
6646-6653
1992
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1
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2
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6
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2
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1
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2
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286620
Ploux
The NADPH-linked acetoacetyl-C ...
Zoogloea ramigera
Eur. J. Biochem.
174
177-182
1988
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-
1
-
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2
7
1
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3
1
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4
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1
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1
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6
1
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6
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1
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1
1
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2
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7
1
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3
1
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1
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1
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6
1
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6
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286619
Fukui
Purification and characterizat ...
Pigeon, Zoogloea ramigera
Biochim. Biophys. Acta
917
365-371
1987
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-
-
-
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1
1
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2
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1
4
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1
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2
1
2
7
1
1
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4
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4
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1
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1
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2
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1
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1
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2
1
2
7
1
1
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286618
Cook
Isolation of rat liver microso ...
Rattus norvegicus
Arch. Biochem. Biophys.
246
206-216
1986
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-
-
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2
1
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1
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1
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1
2
1
2
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1
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3
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3
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2
1
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1
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1
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1
2
1
2
-
1
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286617
Prasad
Rat hepatic microsomal acetoac ...
Rattus norvegicus
J. Biol. Chem.
259
7460-7467
1984
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2
2
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2
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1
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2
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2
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1
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1
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3
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3
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2
2
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2
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1
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1
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1
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286615
Dodds
Acetoacetyl-CoA reductase acti ...
Anser sp., Bos taurus, Gallus gallus, Pigeon, Rattus norvegicus, Saccharomyces cerevisiae
J. Biol. Chem.
256
6282-6290
1981
4
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4
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10
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2
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12
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12
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54
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9
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286614
Saito
An NADP-linked acetoacetyl CoA ...
Azotobacter beijerinckii, Zoogloea ramigera
Arch. Microbiol.
114
211-217
1977
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1
2
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4
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3
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6
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6
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1
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4
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1
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1
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6
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1
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1
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286616
Packter
-
Characterization of acetoacety ...
Streptomyces coelicolor
Biochem. Soc. Trans.
11
598-599
1973
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1
3
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3
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1
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1
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1
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3
1
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286613
Wakil
Studies on the mechanism of fa ...
Pigeon
J. Biol. Chem.
237
687-693
1962
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1
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1
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2
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1
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