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Literature summary for 1.1.1.36 extracted from

  • Fukui, T.; Ito, M.; Saito, T.; Tomita, K.
    Purification and characterization of NADP-linked acetoacetyl-CoA reductase from Zoogloea ramigera I-16-M (1987), Biochim. Biophys. Acta, 917, 365-371.
    View publication on PubMed

General Stability

General Stability Organism
glycerol stabilizes during purification Zoogloea ramigera

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA markedly at concentrations above 0.005 mM and NADPH 0.01 mM, less inhibition when NADPH increases from 0.01 to 1 mM Zoogloea ramigera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25500
-
4 * 25500, SDS-PAGE Zoogloea ramigera
92000
-
gel electrophoresis Zoogloea ramigera

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol during purification Zoogloea ramigera

Organism

Organism UniProt Comment Textmining
Pigeon
-
-
-
Zoogloea ramigera
-
I-16-M
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, DEAE-Sephasrose CL-6B, hydroxyapatite, Red-Sepharose CL-6B chromatography Zoogloea ramigera

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Zoogloea ramigera
-
liver
-
Pigeon
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
412
-
-
Zoogloea ramigera

Storage Stability

Storage Stability Organism
-20°C, 10 mM Tris/HCl buffer, pH 7.5, 10 mM 2-mercaptoethanol, 50% glycerol, several months Zoogloea ramigera
0°C and -20°C, 10 mM Tris/HCl buffer, pH 7.5, 10 mM 2-mercaptoethanol, rather unstable Zoogloea ramigera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + NADPH + H+
-
Zoogloea ramigera (3R)-3-hydroxybutyryl-CoA + NADP+
-
r
acetoacetyl-CoA + NADPH + H+
-
Pigeon (3R)-3-hydroxybutyryl-CoA + NADP+
-
r
D-3-hydroxybutyryl-CoA + NADP+
-
Zoogloea ramigera acetoacetyl-CoA + NADPH
-
r
additional information
-
Pigeon ?
-
?
additional information poor substrate: D-3-hydroxyvaleryl-CoA Zoogloea ramigera ?
-
?
additional information not: L-3-hydroxyacyl-CoA Zoogloea ramigera ?
-
?
additional information not: D-3-hydroxyacyl-CoA's with chain length exceeding 6 Zoogloea ramigera ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 25500, SDS-PAGE Zoogloea ramigera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Zoogloea ramigera

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Zoogloea ramigera
NADP+
-
Pigeon
NADPH specific for Zoogloea ramigera
NADPH specific for Pigeon