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Literature summary for 1.1.1.358 extracted from

  • Yamamura, A.; Maruoka, S.; Ohtsuka, J.; Miyakawa, T.; Nagata, K.; Kataoka, M.; Kitamura, N.; Shimizu, S.; Tanokura, M.
    Expression, purification, crystallization and preliminary X-ray analysis of conjugated polyketone reductase C2 (CPR-C2) from Candida parapsilosis IFO 0708 (2009), Acta Crystallogr. Sect. F, 65, 1145-1148.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Candida parapsilosis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M Tris-HCl pH 8.1 and 23% (w/v) PEG 3350 Candida parapsilosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Candida parapsilosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydropantolactone + NADPH + H+ Candida parapsilosis
-
(R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+ Candida parapsilosis IFO 0708
-
(R)-pantolactone + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Candida parapsilosis Q76L36
-
-
Candida parapsilosis IFO 0708 Q76L36
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose column chromatography and Superdex 75 gel filtration Candida parapsilosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydropantolactone + NADPH + H+
-
Candida parapsilosis (R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+
-
Candida parapsilosis IFO 0708 (R)-pantolactone + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Candida parapsilosis

Synonyms

Synonyms Comment Organism
conjugated polyketone reductase C2
-
Candida parapsilosis
CPR-C2
-
Candida parapsilosis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Candida parapsilosis