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Literature summary for 1.1.1.358 extracted from
- Ketopantoyl-lactone reductase from Candida parapsilosis: purification and characterization as a conjugated polyketone reductase (1989), Biochim. Biophys. Acta, 990, 175-181.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Candida parapsilosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-dehydropantolactone | competitive versus isatin | Candida parapsilosis |  |
| 3,4-Dihydroxy-3-cyclobutene-1,2-dione | uncompetitive | Candida parapsilosis | |
| Al3+ | 83%% inhibition, reduction of 2-dehydropantolactone | Candida parapsilosis | |
| Cd2+ | 97% inhibition, reduction of 2-dehydropantolactone | Candida parapsilosis | |
| Cu2+ | completely inhibits reduction of 2-dehydropantolactone | Candida parapsilosis | |
| cyclohexenediol-1,2,3,4-tetraone | uncompetitive | Candida parapsilosis | |
| Hg2+ | completely inhibits reduction of 2-dehydropantolactone | Candida parapsilosis | |
| iodoacetate | 1 mM, 49% inhibition | Candida parapsilosis | |
| p-chloromercuribenzoate | 0.1 mM, 72% inhibition | Candida parapsilosis | |
| parabanic acid | uncompetitive | Candida parapsilosis | |
| quercetin | 0.1 mM, 94% inhibition | Candida parapsilosis | |
| Zn2+ | completely inhibits reduction of 2-dehydropantolactone | Candida parapsilosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
isatin | pH 7.0, 30°C | Candida parapsilosis | |
| 0.016 | - |
5-methylisatin | pH 7.0, 30°C | Candida parapsilosis | |
| 0.085 | - |
1-methylisatin | pH 7.0, 30°C | Candida parapsilosis | |
| 0.333 | - |
2-dehydropantolactone | pH 7.0, 30°C | Candida parapsilosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
gel filtration | Candida parapsilosis |
| 40000 | - |
polyacrylamide gel electrophoresis | Candida parapsilosis |
| 41600 | - |
1 * 41600, SDS-PAGE | Candida parapsilosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-dehydropantolactone + NADPH + H+ | Candida parapsilosis | - |
(R)-pantolactone + NADP+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida parapsilosis | - |
- |
- |
| Candida parapsilosis IFO 0708 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Candida parapsilosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 173 | - |
pH 7.0, 30°C, substrate: 2-dehydropantolactone | Candida parapsilosis |
| 251 | - |
pH 7.0, 30°C, substrate: isatin | Candida parapsilosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-methylisatin + NADPH + H+ | 86% of the activity compared to isatin | Candida parapsilosis | ? | - |
? | |
| 2-dehydropantolactone + NADPH + H+ | - |
Candida parapsilosis | (R)-pantolactone + NADP+ | - |
ir | |
| 2-dehydropantolactone + NADPH + H+ | 69% of the activity compared to isatin. The stereospecificity for NADPH is not known. Activity with NADH is 2% compared to the activity with NADPH | Candida parapsilosis | (R)-pantolactone + NADP+ | - |
ir | |
| 5-methylisatin + NADPH + H+ | 108% of the activity compared to isatin | Candida parapsilosis | ? | - |
? | |
| camphorquinone + NADPH + H+ | 97% of the activity compared to isatin | Candida parapsilosis | ? | - |
? | |
| isatin + NADPH + H+ | - |
Candida parapsilosis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 41600, SDS-PAGE | Candida parapsilosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ketopantoyl-lactone reductase | - |
Candida parapsilosis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 7.0, 10 min, 58% loss of activity | Candida parapsilosis |
| 60 | - |
pH 7.0, 10 min, 77% loss of activity | Candida parapsilosis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
30°C, 30 min, 33% loss of activity | Candida parapsilosis |
| 6 | 10 | enzyme is stable | Candida parapsilosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | activity with NADH is 2% compared to the activity with NADPH | Candida parapsilosis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
cyclohexenediol-1,2,3,4-tetraone | pH 7.0, 30°C, substrate: isatin | Candida parapsilosis | |
| 0.0014 | - |
3,4-Dihydroxy-3-cyclobutene-1,2-dione | pH 7.0, 30°C, substrate: isatin | Candida parapsilosis | |
| 0.19 | - |
2-dehydropantolactone | pH 7.0, 30°C, substrate: isatin | Candida parapsilosis | |
| 3.14 | - |
parabanic acid | pH 7.0, 30°C, substrate: isatin | Candida parapsilosis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Candida parapsilosis | electrofocussing | - |
6.3 |
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