KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.051 | - |
geraniol | pH 8.5, 22°C | Carpoglyphus lactis | |
0.0595 | - |
NAD+ | pH 8.5, 22°C | Carpoglyphus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40630 | - |
1 * 40630, calculated from sequence | Carpoglyphus lactis |
42800 | - |
1 * 42800, SDS-PAGE | Carpoglyphus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesol + NAD+ | Carpoglyphus lactis | key enzyme in biosynthesis of the alarm pheromone neral | (2E,6E)-farnesal + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carpoglyphus lactis | B2NI94 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Carpoglyphus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31 | - |
pH 8.5, 22°C | Carpoglyphus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesol + NAD+ | key enzyme in biosynthesis of the alarm pheromone neral | Carpoglyphus lactis | (2E,6E)-farnesal + NADH + H+ | - |
? | |
(2E,6E)-farnesol + NAD+ | 50% of the activity compared to geraniol | Carpoglyphus lactis | (2E,6E)-farnesal + NADH + H+ | - |
? | |
3-methyl-2-buten-1-ol + NAD+ | 4.3% of the activity compared to geraniol | Carpoglyphus lactis | 3-methyl-2-butenal + NADH + H+ | - |
? | |
citronellol + NAD+ | 4.3% of the activity compared to geraniol | Carpoglyphus lactis | ? | - |
? | |
geraniol + NAD+ | nerol, the geometrical isomer of geraniol is inactive. The enzyme cannot use NADP+ as cofactor | Carpoglyphus lactis | geranial + NADH + H+ | - |
? | |
additional information | straight-chain alcohols such as 1-octanol, 2-buten-1-ol, 1-butanol and ethanol serve as poor substrates | Carpoglyphus lactis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40630, calculated from sequence | Carpoglyphus lactis |
monomer | 1 * 42800, SDS-PAGE | Carpoglyphus lactis |
Synonyms | Comment | Organism |
---|---|---|
GeDH | - |
Carpoglyphus lactis |
NAD+-farnesol dehydrogenase | - |
Carpoglyphus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Carpoglyphus lactis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 60 | 5°C: about 50% of maximal activity, 60°C: about 50% of maximal activity | Carpoglyphus lactis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2996 | - |
geraniol | pH 8.5, 22°C | Carpoglyphus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Carpoglyphus lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10.5 | pH 8.0: about 75% of maximal activity, pH 10.5: about 35% of maximal activity | Carpoglyphus lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme cannot use NADP+ as cofactor | Carpoglyphus lactis |
General Information | Comment | Organism |
---|---|---|
physiological function | key enzyme in biosynthesis of the alarm pheromone neral | Carpoglyphus lactis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
58400 | - |
geraniol | pH 8.5, 22°C | Carpoglyphus lactis |