Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
4 * 31000, sedimentation equilibrium analysis | Pseudomonas fluorescens |
32000 | - |
4 * 32000, SDS-PAGE | Pseudomonas fluorescens |
115000 | - |
catalytically active form, gel filtration | Pseudomonas fluorescens |
126000 | - |
native protein, gel filtration | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:D-glucose-grown cell | - |
Pseudomonas fluorescens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.023 | - |
using NAD+ as cosubstrate, in 50 mM Tris/HCl buffer pH 8.2, at 20°C | Pseudomonas fluorescens |
0.121 | - |
using NADP+ as cosubstrate, in 50 mM Tris/HCl buffer pH 8.2, at 20°C | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NAD+ | - |
Pseudomonas fluorescens | D-ribulose 5-phosphate + CO2 + NADH + H+ | - |
? | |
6-phospho-D-gluconate + NADP+ | - |
Pseudomonas fluorescens | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 32000, SDS-PAGE | Pseudomonas fluorescens |
homotetramer | 4 * 31000, sedimentation equilibrium analysis | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
6-phospho-D-gluconate dehydrogenase (decarboxylating) | - |
Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme is active with both NAD+ and NADP+ | Pseudomonas fluorescens | |
NADP+ | the enzyme is active with both NAD+ and NADP+. The rate of reaction with NADP+ is 20%, of the rate with NAD+ | Pseudomonas fluorescens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas fluorescens | isoelectric focusing | - |
4.6 |