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Literature summary for 1.1.1.35 extracted from

  • Segawa, T.; Nomura, K.H.; Villanueva, S.Y.; Saito, M.; Nomura, K.; Gloriani, N.G.; Yoshida, S.
    Identification of leptospiral 3-hydroxyacyl-CoA dehydrogenase released in the urine of infected hamsters (2014), BMC Microbiol., 14, 132.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
diagnostics the enzyme can be used as marker for early immunonological detection of infection by Leptospira in urine and of leptospirosis, a global zoonosis caused by pathogenic Leptospira Leptospira interrogans serovar Copenhageni

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme in Escherichia coli Leptospira interrogans serovar Copenhageni

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Leptospira interrogans serovar Copenhageni 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47865
-
-
Leptospira interrogans serovar Copenhageni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxyacyl-CoA + NAD+ Leptospira interrogans serovar Copenhageni
-
3-oxoacyl-CoA + NADH + H+
-
?
(S)-3-hydroxyacyl-CoA + NAD+ Leptospira interrogans serovar Copenhageni Fiocruz LV4231
-
3-oxoacyl-CoA + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Leptospira interrogans serovar Copenhageni Q72M90
-
-
Leptospira interrogans serovar Copenhageni Fiocruz LV4231 Q72M90
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by Co2+ affinity chromatography Leptospira interrogans serovar Copenhageni

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme detection in urine of infected Mesocricetus auratus: leptospiral protein, 3-hydroxyacyl-CoA dehydrogenase (HADH), is found to be excreted in the urine of hamsters during the early phase of infection Leptospira interrogans serovar Copenhageni
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxyacyl-CoA + NAD+
-
Leptospira interrogans serovar Copenhageni 3-oxoacyl-CoA + NADH + H+
-
?
(S)-3-hydroxyacyl-CoA + NAD+
-
Leptospira interrogans serovar Copenhageni Fiocruz LV4231 3-oxoacyl-CoA + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 52000, recombinant His-tagged enzyme, SDS-PAGE, x * 47865, sequence calculation Leptospira interrogans serovar Copenhageni
? x * 60000, native enzyme isolated from hamster urine, SDS-PAGE Leptospira interrogans serovar Copenhageni

Synonyms

Synonyms Comment Organism
HADH
-
Leptospira interrogans serovar Copenhageni
LIC13300
-
Leptospira interrogans serovar Copenhageni

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Leptospira interrogans serovar Copenhageni

General Information

General Information Comment Organism
metabolism the enzyme specifically catalyzes the third step of beta oxidation Leptospira interrogans serovar Copenhageni
physiological function the enzyme is classified as an oxidoreductase in fatty acid metabolic processes. It specifically catalyzes the third step of beta oxidation. Long-chain fatty acids are utilized by Leptospira as the sole carbon source and are metabolized by beta-oxidation. Therefore, a large amount of HADH may be produced intracellularly and released to get carbons and energy by oxidizing free fatty acid Leptospira interrogans serovar Copenhageni