KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
ethyl 2-methylacetoacetate | at pH 7.0 and 30°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31800 | - |
x * 31800, estimated from SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli BL21-(DE3) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Matrex Red-A column chromatography, Sephacryl S-200 gel filtration, and hypatite C cholumn chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.3 | - |
purified enzyme, at pH 7.0 and 30°C | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, potassium buffer, several weeks, no loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethyl 2-acetylpent-4-enoate + NADPH + H+ | 250% activity compared to ethyl 2-methylacetoacetate | Escherichia coli | ethyl (2R)-2-[(1S)-1-hydroxyethyl]pent-4-enoate + NADP+ | - |
? | |
ethyl 2-ethyl-3-oxobutanoate + NADPH + H+ | 120% activity compared to ethyl 2-methylacetoacetate | Escherichia coli | ethyl (2R,3S)-2-ethyl-3-hydroxybutanoate + NADP+ | - |
? | |
ethyl 2-methylacetoacetate + NADH + H+ | 7% activity with NADH compared to NADPH | Escherichia coli | ethyl (2R)-methyl-(3S)-hydroxybutanoate + NAD+ | - |
? | |
ethyl 2-methylacetoacetate + NADPH + H+ | - |
Escherichia coli | ethyl (2R)-methyl-(3S)-hydroxybutanoate + NADP+ | - |
? | |
ethyl acetoacetate + NADPH + H+ | 53% activity compared to ethyl 2-methylacetoacetate | Escherichia coli | ethyl (3S)-3-hydroxybutanoate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31800, estimated from SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
2,5-diketo-D-gluconate reductase | - |
Escherichia coli |
2,5-DKG reductase | - |
Escherichia coli |
YqhE | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
ethyl 2-methylacetoacetate | at pH 7.0 and 30°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | - |
5.9 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme reduces 2,5-didehydro-D-gluconate, a key step in the microbial synthesis of vitamin C | Escherichia coli |