Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with genes ldh1837 and ldh2043 | Leuconostoc mesenteroides subsp. mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-malate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides | - |
oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | - |
oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 | - |
oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 | - |
oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 | - |
oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 | - |
oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 | - |
oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 | - |
oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 | - |
oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides 37Y | - |
oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 | - |
oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides subsp. mesenteroides | Q03V58 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides 37Y | Q03V58 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | Q03V58 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 | Q03V58 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 | Q03V58 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 | Q03V58 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 | Q03V58 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 | Q03V58 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 | Q03V58 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 | Q03V58 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 | Q03V58 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 | Q03V58 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Leuconostoc mesenteroides subsp. mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides 37Y | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 | 2-hydroxybutyrate + NAD+ | - |
r | |
D-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides | oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 | oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 | oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 | oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 | oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 | oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 | oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 | oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides 37Y | oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 | oxaloacetate + NADH + H+ | - |
r | |
D-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 | oxaloacetate + NADH + H+ | - |
r | |
additional information | the recombinant the proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides | ? | - |
- |
|
additional information | the recombinant the proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | ? | - |
- |
|
additional information | the recombinant the proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 | ? | - |
- |
|
additional information | the recombinant the proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 | ? | - |
- |
|
additional information | the recombinant the proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 | ? | - |
- |
|
additional information | the recombinant the proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 | ? | - |
- |
|
additional information | the recombinant the proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 | ? | - |
- |
|
additional information | the recombinant the proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 | ? | - |
- |
|
additional information | the recombinant the proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 | ? | - |
- |
|
additional information | the recombinant the proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides 37Y | ? | - |
- |
|
additional information | the recombinant the proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 | ? | - |
- |
|
additional information | the recombinant the proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 | ? | - |
- |
|
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides | D-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | D-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 | D-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 | D-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 | D-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 | D-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 | D-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 | D-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 | D-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides 37Y | D-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 | D-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 | D-malate + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
D-malate dehydrogenases | - |
Leuconostoc mesenteroides subsp. mesenteroides |
D-MDH | - |
Leuconostoc mesenteroides subsp. mesenteroides |
ldh1837 | - |
Leuconostoc mesenteroides subsp. mesenteroides |
LEUM_1837 | - |
Leuconostoc mesenteroides subsp. mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Leuconostoc mesenteroides subsp. mesenteroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides | |
NADH | - |
Leuconostoc mesenteroides subsp. mesenteroides |
General Information | Comment | Organism |
---|---|---|
metabolism | in Leuconostoc mesenteroides strain ATCC 8293, which lacks an L-ldh gene, L-lactate is produced through sequential enzymatic conversions from phosphoenolpyruvate to oxaloacetate, then L-malate, and finally L-lactate by phosphoenolpyruvate carboxylase (PEPC, gene ppcA, UniProt ID Q03VI7, LEUM_1694), L-MDH, and malolactic enzyme (MLE, UniProt ID Q03XG6, LEUM_1005), respectively | Leuconostoc mesenteroides subsp. mesenteroides |