Cloned (Comment) | Organism |
---|---|
expression of the His-tagged catalytic domain of the enzyme, residues E416-F887, in Escherichia coli | Phaedon cochleariae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxygeraniol | competitive inhibition mechanism, binds at the catalytic site, docking experiments, overview | Phaedon cochleariae | |
fluvastatin | - |
Phaedon cochleariae | |
additional information | no inhibition by geraniol or by its glucoside and thioglucoside | Phaedon cochleariae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | a transmembrane enzyme | Phaedon cochleariae | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54500 | - |
x * 54500, recombinant His-tagged catalytic domain, SDS-PAGE | Phaedon cochleariae |
97100 | - |
2 * 97100, about, sequence calculation | Phaedon cochleariae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxymethylglutaryl-CoA + NADPH + H+ | Phaedon cochleariae | HMGR is the key regulatory enzyme of the mevalonate pathway and also the iridoid biosynthesis, it is highly regulated itself, HMGR may represent a regulator in maintenance of homeostasis between de novo produced and sequestered intermediates of iridoid metabolism, overview | mevalonate + NADP+ + CoA | - |
? | |
additional information | Phaedon cochleariae | HMGR activity is not only diminished in iridoid producers but most likely prevalent within the Chrysomelina subtribe and also within the insecta | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaedon cochleariae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme sequence contains a glycosylation site | Phaedon cochleariae |
phosphoprotein | the enzyme sequence contains a phosphorylation site | Phaedon cochleariae |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged catalytic domain from Escherichia coli by nickel affinity chromatography | Phaedon cochleariae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Phaedon cochleariae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxymethylglutaryl-CoA + NADPH + H+ | HMGR is the key regulatory enzyme of the mevalonate pathway and also the iridoid biosynthesis, it is highly regulated itself, HMGR may represent a regulator in maintenance of homeostasis between de novo produced and sequestered intermediates of iridoid metabolism, overview | Phaedon cochleariae | mevalonate + NADP+ + CoA | - |
? | |
hydroxymethylglutaryl-CoA + NADPH + H+ | the enzyme utilizes two molecules of NADPH to mediate the four-electron reduction of HMG-CoA to the carboxylic acid mevalonate, homology modeling of the catalytic domain | Phaedon cochleariae | mevalonate + NADP+ + CoA | - |
? | |
additional information | HMGR activity is not only diminished in iridoid producers but most likely prevalent within the Chrysomelina subtribe and also within the insecta | Phaedon cochleariae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54500, recombinant His-tagged catalytic domain, SDS-PAGE | Phaedon cochleariae |
dimer | 2 * 97100, about, sequence calculation | Phaedon cochleariae |
More | homology modeling of the catalytic domain | Phaedon cochleariae |
Synonyms | Comment | Organism |
---|---|---|
HMGR | - |
Phaedon cochleariae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Phaedon cochleariae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 35 | - |
Phaedon cochleariae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
MOPS buffer | Phaedon cochleariae |
7.5 | - |
phosphate buffer | Phaedon cochleariae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Phaedon cochleariae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Phaedon cochleariae | sequence calculation | - |
5.9 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00022 | - |
pH 6.8, 30°C | Phaedon cochleariae | fluvastatin | |
1.2 | - |
pH 6.8, 30°C, recombinant catalytic domain | Phaedon cochleariae | 8-hydroxygeraniol |