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Literature summary for 1.1.1.34 extracted from

  • Li, S.; Friesen, J.A.; Fei, H.; Ding, X.; Borst, D.W.
    The lobster mandibular organ produces soluble and membrane-bound forms of 3-hydroxy-3-methylglutaryl-CoA reductase (2004), Biochem. J., 381, 831-840.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
isozyme HMGR1, DNA and amino acid sequence determination and analysis, functional expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system as His-tagged enzyme Homarus americanus
isozyme HMGR2, DNA and amino acid sequence determination and analysis Homarus americanus

Inhibitors

Inhibitors Comment Organism Structure
lovastatin
-
Homarus americanus
mevalonic acid
-
Homarus americanus
additional information no inhibition by farnesoate and farnesoic acid Homarus americanus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound, isozyme HMGR2, about 25% of total enzyme content Homarus americanus 16020
-
soluble isozyme HMGR1, about 75% of total enzyme content Homarus americanus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
-
Homarus americanus
140000
-
isozyme HMGR1, minor peak, gel filtration Homarus americanus
540000
-
isozyme HMGR1, major peak, gel filtration Homarus americanus

Organism

Organism UniProt Comment Textmining
Homarus americanus Q7YT62 lobster, 2 enzyme forms: a soluble and a membrane-bound enzyme
-
Homarus americanus Q7YT63 lobster, 2 enzyme forms: a soluble isozyme HMGR1 and a membrane-bound isozyme HMGR2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the isozyme is not regulated by phosphorylation Homarus americanus
additional information the isozyme is probably not regulated by phosphorylation Homarus americanus

Purification (Commentary)

Purification (Comment) Organism
native soluble isozyme HMGR1 from mandibular organ by ultracentrifugation, anion exchange chromatography, ultrafiltration and gel filtration, recombinant enzyme from Sf9 insect cells by nickel affinity chromatography Homarus americanus

Source Tissue

Source Tissue Comment Organism Textmining
mandibular organ
-
Homarus americanus
-
additional information very low content in other tissues, higher enzyme activity in male organisms than in female, tissue distribution overview Homarus americanus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0006
-
about, native cells Homarus americanus
60
-
recombinant Sf9 cells Homarus americanus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
Homarus americanus (R)-mevalonate + CoA + 2 NADP+
-
r

Subunits

Subunits Comment Organism
oligomer 1 x 69000, recombinant His-tagged isozyme HMGR1, SDS-PAGE Homarus americanus

Synonyms

Synonyms Comment Organism
3-hydroxy-3-methylglutaryl CoA reductase
-
Homarus americanus
HMGR
-
Homarus americanus
HMGR1
-
Homarus americanus
HMGR2
-
Homarus americanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homarus americanus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000045
-
lovastatin pH 7.4, recombinant isozyme HMGR1 Homarus americanus
0.081
-
mevalonic acid pH 7.4, recombinant isozyme HMGR1 Homarus americanus